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Article: Comparison of vascular relaxation, lipolysis and glucose uptake by peroxisome proliferator-activated receptor-γ activation in + db/+ m and + db/+ db mice
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TitleComparison of vascular relaxation, lipolysis and glucose uptake by peroxisome proliferator-activated receptor-γ activation in + db/+ m and + db/+ db mice
 
AuthorsSeto, SW3
Lam, TY3
Leung, GPH1
Au, ALS3
Ngai, SM3
Chan, SW2
Kwan, YW3 3
 
Keywords+ db/+ db mice
Aortic relaxation
Glucose uptake
Lipolysis
Peroxisome proliferator-activated receptor-γ
 
Issue Date2007
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
 
CitationEuropean Journal Of Pharmacology, 2007, v. 572 n. 1, p. 40-48 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ejphar.2007.05.070
 
AbstractIn this study, we determined the in vitro effect of peroxisome proliferator-activated receptor-γ (PPAR-γ) activation on the aortic relaxation, lipolysis and insulin-induced [ 3H]-glucose uptake of the abdominal (omental) adipocytes of the non-diabetic (+ db/+ m) and obese/diabetic (+ db/+ db) mice. The expression of PPAR-γ (mRNA and protein) in aorta and adipose tissues was evaluated and compared. Cumulative application of ciglitazone, pioglitazone and troglitazone (PPAR-γ agonists) caused a concentration-dependent aortic relaxation (sensitive to 2-chloro-5-nitro-N-phenylbenzamide (GW9662) (1 μM, a selective PPAR-γ antagonist) and N ω-nitro-l-arginine methyl ester (l-NAME) (20 μM, a nitric oxide synthase inhibitor)) with a maximum relaxation of ∼ 30% (3 μM) in + db/+ m mice, whereas no relaxation was observed in + db/+ db mice. All PPAR-γ agonists examined did not alter the basal lipolysis of both species, but forskolin caused a concentration-dependent lipolysis, with a greater magnitude observed in + db/+ m mice. Insulin (0.1 and 1 μM) caused an enhancement of [ 3H]-glucose uptake into adipocytes with a greater magnitude in + db/+ m mice. In contrast, none of the PPAR-γ agonists tested (0.1, 1 and 10 μM) altered the basal and the insulin (0.1 μM)-induced [ 3H]-glucose uptake into adipocytes of both species. In addition, there was no difference in PPAR-γ expression (mRNA and protein) in the aorta and adipose tissues between the species. In conclusion, our results demonstrate that PPAR-γ is present in the abdominal (omental) adipose tissue and thoracic aorta. An acute activation of PPAR-γ produced a small (∼ 30%) aortic relaxation (nitric oxide/endothelium-dependent) of + db/+ m mice. However, all PPAR-γ agonists examined have no acute effect on lipolysis and the insulin-induced glucose uptake into adipocytes of both + db/+ m and + db/+ db mice. © 2007 Elsevier B.V. All rights reserved.
 
ISSN0014-2999
2012 Impact Factor: 2.592
2012 SCImago Journal Rankings: 0.832
 
DOIhttp://dx.doi.org/10.1016/j.ejphar.2007.05.070
 
ISI Accession Number IDWOS:000250191200005
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSeto, SW
 
dc.contributor.authorLam, TY
 
dc.contributor.authorLeung, GPH
 
dc.contributor.authorAu, ALS
 
dc.contributor.authorNgai, SM
 
dc.contributor.authorChan, SW
 
dc.contributor.authorKwan, YW
 
dc.date.accessioned2010-09-06T08:05:04Z
 
dc.date.available2010-09-06T08:05:04Z
 
dc.date.issued2007
 
dc.description.abstractIn this study, we determined the in vitro effect of peroxisome proliferator-activated receptor-γ (PPAR-γ) activation on the aortic relaxation, lipolysis and insulin-induced [ 3H]-glucose uptake of the abdominal (omental) adipocytes of the non-diabetic (+ db/+ m) and obese/diabetic (+ db/+ db) mice. The expression of PPAR-γ (mRNA and protein) in aorta and adipose tissues was evaluated and compared. Cumulative application of ciglitazone, pioglitazone and troglitazone (PPAR-γ agonists) caused a concentration-dependent aortic relaxation (sensitive to 2-chloro-5-nitro-N-phenylbenzamide (GW9662) (1 μM, a selective PPAR-γ antagonist) and N ω-nitro-l-arginine methyl ester (l-NAME) (20 μM, a nitric oxide synthase inhibitor)) with a maximum relaxation of ∼ 30% (3 μM) in + db/+ m mice, whereas no relaxation was observed in + db/+ db mice. All PPAR-γ agonists examined did not alter the basal lipolysis of both species, but forskolin caused a concentration-dependent lipolysis, with a greater magnitude observed in + db/+ m mice. Insulin (0.1 and 1 μM) caused an enhancement of [ 3H]-glucose uptake into adipocytes with a greater magnitude in + db/+ m mice. In contrast, none of the PPAR-γ agonists tested (0.1, 1 and 10 μM) altered the basal and the insulin (0.1 μM)-induced [ 3H]-glucose uptake into adipocytes of both species. In addition, there was no difference in PPAR-γ expression (mRNA and protein) in the aorta and adipose tissues between the species. In conclusion, our results demonstrate that PPAR-γ is present in the abdominal (omental) adipose tissue and thoracic aorta. An acute activation of PPAR-γ produced a small (∼ 30%) aortic relaxation (nitric oxide/endothelium-dependent) of + db/+ m mice. However, all PPAR-γ agonists examined have no acute effect on lipolysis and the insulin-induced glucose uptake into adipocytes of both + db/+ m and + db/+ db mice. © 2007 Elsevier B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationEuropean Journal Of Pharmacology, 2007, v. 572 n. 1, p. 40-48 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ejphar.2007.05.070
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ejphar.2007.05.070
 
dc.identifier.epage48
 
dc.identifier.hkuros157509
 
dc.identifier.isiWOS:000250191200005
 
dc.identifier.issn0014-2999
2012 Impact Factor: 2.592
2012 SCImago Journal Rankings: 0.832
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid17603034
 
dc.identifier.scopuseid_2-s2.0-34548495791
 
dc.identifier.spage40
 
dc.identifier.urihttp://hdl.handle.net/10722/80326
 
dc.identifier.volume572
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofEuropean Journal of Pharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsEuropean Journal of Pharmacology. Copyright © Elsevier BV.
 
dc.subject+ db/+ db mice
 
dc.subjectAortic relaxation
 
dc.subjectGlucose uptake
 
dc.subjectLipolysis
 
dc.subjectPeroxisome proliferator-activated receptor-γ
 
dc.titleComparison of vascular relaxation, lipolysis and glucose uptake by peroxisome proliferator-activated receptor-γ activation in + db/+ m and + db/+ db mice
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Polytechnic University
  3. Chinese University of Hong Kong