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Article: Panax quinquefolium saponins protects low density lipoproteins from oxidation

TitlePanax quinquefolium saponins protects low density lipoproteins from oxidation
Authors
KeywordsAntioxidant
Endothelium- dependent relaxation
LDL oxidation
Panax quinquefolium saponin
Issue Date1998
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1998, v. 64 n. 1, p. 53-62 How to Cite?
AbstractOxidized low-density lipoprotein (Ox-LDL) is believed to be involved in the pathogenesis of atherosclerosis. Panax quinquefolium saponins (PQS) are extracted from the stems and leaves of the North American form of ginseng, Panax quinquefolium. Earlier studies have suggested that this extract improves the lipid profile of hyperlipidemic rats and has antioxidant properties in cultured rat cardiac myocytes. The aims of the present study were to investigate the potential of PQS in reducing LDL oxidation as well as limiting the ability of Ox-LDL to impair endothelium-dependent relaxation in rat aortic rings. LDL was isolated from the plasma of healthy human donors by sequential ultracentrifugation. Native LDL (0.2 or 0.3 mg/ml) was incubated with PQS (0.25-1 mg/ml) for 30 min at 20°C. For comparison, vitamin C (50 μM) was added in place of PQS. Oxidative modification was initiated with 5 μM CuSO 4 at 37°C for 0-24 h. In our hands, PQS concentration-dependently reduced lipid peroxide levels as measured by the amount of thiobarbituric acid reactive substances formed. This range of PQS also retarded the alterations in relative electrophoretic mobility of Ox-LDL in a similar manner. Furthermore, measurement of phospholipid fractions content indicated that PQS could reduce the conversion of phosphatidylcholine to lysophosphatidylcholine in Ox-LDL. Functional studies demonstrated that PQS- pretreated Ox-LDL was less potent than untreated Ox-LDL at impairing endothelium-dependent relaxation in rat aortic rings. In conclusion, our results suggest that PQS has antioxidant properties and that reduction of LDL oxidation by PQS may provide a protective effect against the detrimental actions of Ox-LDL.
Persistent Identifierhttp://hdl.handle.net/10722/80314
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Jen_HK
dc.contributor.authorHuang, Men_HK
dc.contributor.authorTeoh, Hen_HK
dc.contributor.authorMan, RYKen_HK
dc.date.accessioned2010-09-06T08:04:56Z-
dc.date.available2010-09-06T08:04:56Z-
dc.date.issued1998en_HK
dc.identifier.citationLife Sciences, 1998, v. 64 n. 1, p. 53-62en_HK
dc.identifier.issn0024-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80314-
dc.description.abstractOxidized low-density lipoprotein (Ox-LDL) is believed to be involved in the pathogenesis of atherosclerosis. Panax quinquefolium saponins (PQS) are extracted from the stems and leaves of the North American form of ginseng, Panax quinquefolium. Earlier studies have suggested that this extract improves the lipid profile of hyperlipidemic rats and has antioxidant properties in cultured rat cardiac myocytes. The aims of the present study were to investigate the potential of PQS in reducing LDL oxidation as well as limiting the ability of Ox-LDL to impair endothelium-dependent relaxation in rat aortic rings. LDL was isolated from the plasma of healthy human donors by sequential ultracentrifugation. Native LDL (0.2 or 0.3 mg/ml) was incubated with PQS (0.25-1 mg/ml) for 30 min at 20°C. For comparison, vitamin C (50 μM) was added in place of PQS. Oxidative modification was initiated with 5 μM CuSO 4 at 37°C for 0-24 h. In our hands, PQS concentration-dependently reduced lipid peroxide levels as measured by the amount of thiobarbituric acid reactive substances formed. This range of PQS also retarded the alterations in relative electrophoretic mobility of Ox-LDL in a similar manner. Furthermore, measurement of phospholipid fractions content indicated that PQS could reduce the conversion of phosphatidylcholine to lysophosphatidylcholine in Ox-LDL. Functional studies demonstrated that PQS- pretreated Ox-LDL was less potent than untreated Ox-LDL at impairing endothelium-dependent relaxation in rat aortic rings. In conclusion, our results suggest that PQS has antioxidant properties and that reduction of LDL oxidation by PQS may provide a protective effect against the detrimental actions of Ox-LDL.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_HK
dc.relation.ispartofLife Sciencesen_HK
dc.rightsLife Sciences. Copyright © Elsevier Inc.en_HK
dc.subjectAntioxidanten_HK
dc.subjectEndothelium- dependent relaxationen_HK
dc.subjectLDL oxidationen_HK
dc.subjectPanax quinquefolium saponinen_HK
dc.titlePanax quinquefolium saponins protects low density lipoproteins from oxidationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=64&issue=1&spage=53&epage=62&date=1999&atitle=Panax+quinquefolium+saponins+protects+low+density+lipoproteins+from+oxidationen_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0024-3205(98)00533-5en_HK
dc.identifier.pmid10027742-
dc.identifier.scopuseid_2-s2.0-0032573627en_HK
dc.identifier.hkuros41172en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032573627&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume64en_HK
dc.identifier.issue1en_HK
dc.identifier.spage53en_HK
dc.identifier.epage62en_HK
dc.identifier.isiWOS:000077406900006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, J=12796026200en_HK
dc.identifier.scopusauthoridHuang, M=55198346200en_HK
dc.identifier.scopusauthoridTeoh, H=7003816542en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK

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