File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone from cigarette smoke stimulates colon cancer growth via β-adrenoceptors

Title4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone from cigarette smoke stimulates colon cancer growth via β-adrenoceptors
Authors
Issue Date2005
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 2005, v. 65 n. 12, p. 5272-5277 How to Cite?
AbstractCigarette smoking is a risk factor for colorectal cancer. It is suggested that 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a tobacco-specific nitrosamine, mediates the carcinogenic action of cigarette smoking by promoting cancer growth. In the present study, the proliferative response of a cultured colon cancer cell line HT-29 to NNK was determined. It was found that NNK dose-dependently stimulated HT-29 cell proliferation. In this regard, the stimulatory action of NNK was abolished by atenolol and ICI 118,551, a β 1- and β 2-selective antagonist, respectively. In addition, cell growth was stimulated by the nonselective adrenergic agonist, noradrenaline, and more effectively by the β-selective agonist, isoproterenol. The second message cyclic AMP level for β-adrenoceptor activation was elevated by isoproterenol and NNK treatment. These agents also up-regulated cyclooxygenase-2 expression, cytosolic phospholipase A 2 expression, and prostaglandin E 2 release. β 2- adrenoceptor blockade with ICI 118,551, in contrast, significantly decreased cyclooxygenase-2 expression, cytosolic phospholipase A 2 expression and prostaglandin E 2 release induced by NNK and isoproterenol. To conclude, it is proposed that NNK stimulates HT-29 cell proliferation through β-adrenoceptors, preferentially β 2 receptors. Activation of the β-adrenoceptors, and the consequent cyclic AMP elevation coupled with the downstream arachidonic acid pathway, is perhaps an important mechanistic cascade in the promotion of colon cancer growth. These findings partly elucidate the carcinogenic actions of cigarette smoke and shed new light on the novel modulatory role of β-adrenoceptors in the development of colon cancer. © 2005 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/80309
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, WKKen_HK
dc.contributor.authorWong, HPSen_HK
dc.contributor.authorLuo, SWen_HK
dc.contributor.authorChan, Ken_HK
dc.contributor.authorHuang, FYen_HK
dc.contributor.authorHui, MKCen_HK
dc.contributor.authorLam, EKYen_HK
dc.contributor.authorShin, VYen_HK
dc.contributor.authorYe, YNen_HK
dc.contributor.authorYang, YHen_HK
dc.contributor.authorCho, CHen_HK
dc.date.accessioned2010-09-06T08:04:53Z-
dc.date.available2010-09-06T08:04:53Z-
dc.date.issued2005en_HK
dc.identifier.citationCancer Research, 2005, v. 65 n. 12, p. 5272-5277en_HK
dc.identifier.issn0008-5472en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80309-
dc.description.abstractCigarette smoking is a risk factor for colorectal cancer. It is suggested that 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a tobacco-specific nitrosamine, mediates the carcinogenic action of cigarette smoking by promoting cancer growth. In the present study, the proliferative response of a cultured colon cancer cell line HT-29 to NNK was determined. It was found that NNK dose-dependently stimulated HT-29 cell proliferation. In this regard, the stimulatory action of NNK was abolished by atenolol and ICI 118,551, a β 1- and β 2-selective antagonist, respectively. In addition, cell growth was stimulated by the nonselective adrenergic agonist, noradrenaline, and more effectively by the β-selective agonist, isoproterenol. The second message cyclic AMP level for β-adrenoceptor activation was elevated by isoproterenol and NNK treatment. These agents also up-regulated cyclooxygenase-2 expression, cytosolic phospholipase A 2 expression, and prostaglandin E 2 release. β 2- adrenoceptor blockade with ICI 118,551, in contrast, significantly decreased cyclooxygenase-2 expression, cytosolic phospholipase A 2 expression and prostaglandin E 2 release induced by NNK and isoproterenol. To conclude, it is proposed that NNK stimulates HT-29 cell proliferation through β-adrenoceptors, preferentially β 2 receptors. Activation of the β-adrenoceptors, and the consequent cyclic AMP elevation coupled with the downstream arachidonic acid pathway, is perhaps an important mechanistic cascade in the promotion of colon cancer growth. These findings partly elucidate the carcinogenic actions of cigarette smoke and shed new light on the novel modulatory role of β-adrenoceptors in the development of colon cancer. © 2005 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_HK
dc.relation.ispartofCancer Researchen_HK
dc.title4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone from cigarette smoke stimulates colon cancer growth via β-adrenoceptorsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=65&spage=5272&epage=5277&date=2005&atitle=4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone+from+cigarette+smoke+stimulates+colon+cancer+growth+via+beta-adrenoceptorsen_HK
dc.identifier.emailWong, HPS:hpswong@hkusua.hku.hken_HK
dc.identifier.authorityWong, HPS=rp00808en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1158/0008-5472.CAN-05-0205en_HK
dc.identifier.pmid15958573-
dc.identifier.scopuseid_2-s2.0-20444449329en_HK
dc.identifier.hkuros157312en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20444449329&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume65en_HK
dc.identifier.issue12en_HK
dc.identifier.spage5272en_HK
dc.identifier.epage5277en_HK
dc.identifier.isiWOS:000229734300040-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWu, WKK=18345422600en_HK
dc.identifier.scopusauthoridWong, HPS=8644138100en_HK
dc.identifier.scopusauthoridLuo, SW=55254455200en_HK
dc.identifier.scopusauthoridChan, K=7406033916en_HK
dc.identifier.scopusauthoridHuang, FY=8644138400en_HK
dc.identifier.scopusauthoridHui, MKC=8644138500en_HK
dc.identifier.scopusauthoridLam, EKY=8644138600en_HK
dc.identifier.scopusauthoridShin, VY=7003491170en_HK
dc.identifier.scopusauthoridYe, YN=7401627402en_HK
dc.identifier.scopusauthoridYang, YH=7409390524en_HK
dc.identifier.scopusauthoridCho, CH=7403100461en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats