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Article: Homocysteine stimulates the production and secretion of cholesterol in hepatic cells

TitleHomocysteine stimulates the production and secretion of cholesterol in hepatic cells
Authors
Issue Date1998
PublisherElsevier BV.
Citation
Biochimica Et Biophysica Acta - Lipids And Lipid Metabolism, 1998, v. 1393 n. 2-3, p. 317-324 How to Cite?
AbstractHomocysteinemia and hypercholesterolemia are important risk factors associated with the occurrence of arteriosclerotic vascular diseases. A positive correlation between plasma levels of homocysteine and cholesterol was found in homocysteinemic patients as well as in experimental animals. In the present study, the effect of homocysteine on the production and secretion of cholesterol in human hepatoma cell line HepG2 cells was investigated. When cells were incubated with 4 mM homocysteine, the amounts of total cholesterol produced as well as the cholesterol secreted by these cells were significantly increased (from 32±5 to 74±5 nmol/mg cellular protein). Further biochemical analyses revealed that the increase in cholesterol was resulted from an enhancement in the production and secretion of the unesterified cholesterol with no concomitant change in the level of cholesteryl esters. The activity of intracellular 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was markedly elevated by 131% and 190% after cells were incubated with homocysteine for 24 and 48 h. Homocysteine also stimulated the secretion of apo B100 by HepG2 cells (from 0.84±0.11 to 1.37±0.12 μg apolipoprotein B/mg cellular protein). Our results demonstrate that homocysteine stimulates the production and secretion of cholesterol and apolipoprotein B100 in HepG2 cells. The increase in the production of cholesterol induced by homocysteine may contribute to the pathogenesis of arteriosclerosis. Copyright (C) 1998 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/80298
ISSN
2000 Impact Factor: 2.973
References

 

DC FieldValueLanguage
dc.contributor.authorO, Ken_HK
dc.contributor.authorLynn, EGen_HK
dc.contributor.authorChung, YHen_HK
dc.contributor.authorSiow, YLen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorChoy, PCen_HK
dc.date.accessioned2010-09-06T08:04:45Z-
dc.date.available2010-09-06T08:04:45Z-
dc.date.issued1998en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Lipids And Lipid Metabolism, 1998, v. 1393 n. 2-3, p. 317-324en_HK
dc.identifier.issn0005-2760en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80298-
dc.description.abstractHomocysteinemia and hypercholesterolemia are important risk factors associated with the occurrence of arteriosclerotic vascular diseases. A positive correlation between plasma levels of homocysteine and cholesterol was found in homocysteinemic patients as well as in experimental animals. In the present study, the effect of homocysteine on the production and secretion of cholesterol in human hepatoma cell line HepG2 cells was investigated. When cells were incubated with 4 mM homocysteine, the amounts of total cholesterol produced as well as the cholesterol secreted by these cells were significantly increased (from 32±5 to 74±5 nmol/mg cellular protein). Further biochemical analyses revealed that the increase in cholesterol was resulted from an enhancement in the production and secretion of the unesterified cholesterol with no concomitant change in the level of cholesteryl esters. The activity of intracellular 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was markedly elevated by 131% and 190% after cells were incubated with homocysteine for 24 and 48 h. Homocysteine also stimulated the secretion of apo B100 by HepG2 cells (from 0.84±0.11 to 1.37±0.12 μg apolipoprotein B/mg cellular protein). Our results demonstrate that homocysteine stimulates the production and secretion of cholesterol and apolipoprotein B100 in HepG2 cells. The increase in the production of cholesterol induced by homocysteine may contribute to the pathogenesis of arteriosclerosis. Copyright (C) 1998 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV.en_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Lipids and Lipid Metabolismen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subject.meshApolipoprotein B-100en_HK
dc.subject.meshApolipoproteins B - biosynthesis - secretionen_HK
dc.subject.meshCarbon Radioisotopesen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCholesterol - biosynthesis - secretionen_HK
dc.subject.meshHomocysteine - blood - isolation & purification - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydroxymethylglutaryl CoA Reductases - metabolismen_HK
dc.subject.meshLiver - drug effects - metabolismen_HK
dc.subject.meshTime Factorsen_HK
dc.titleHomocysteine stimulates the production and secretion of cholesterol in hepatic cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=55303&spage=1&epage=8&date=1998&atitle=Homocysteine+stimulates+the+production+and+secretion+of+cholesterol+in+hepatic+cellsen_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0005-2760(98)00086-1en_HK
dc.identifier.pmid9748642-
dc.identifier.scopuseid_2-s2.0-0031692918en_HK
dc.identifier.hkuros45082en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031692918&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1393en_HK
dc.identifier.issue2-3en_HK
dc.identifier.spage317en_HK
dc.identifier.epage324en_HK
dc.identifier.scopusauthoridO, K=7006328603en_HK
dc.identifier.scopusauthoridLynn, EG=7004176471en_HK
dc.identifier.scopusauthoridChung, YH=7404388012en_HK
dc.identifier.scopusauthoridSiow, YL=7003336463en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK
dc.identifier.scopusauthoridChoy, PC=7006633002en_HK
dc.identifier.citeulike10445809-

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