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Article: Ganoderma lucidum inhibits inducible nitric oxide synthase expression in macrophages

TitleGanoderma lucidum inhibits inducible nitric oxide synthase expression in macrophages
Authors
KeywordsHerbal medicine
Inducible nitric oxide synthase
Nitric oxide
Oxidative stress
Issue Date2005
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177
Citation
Molecular And Cellular Biochemistry, 2005, v. 275 n. 1-2, p. 165-171 How to Cite?
AbstractNitric oxide (NO) is a principal mediator in many physiological and pathological processes. Overproduction of NO via the inducible nitric oxide synthase (iNOS) has cytotoxic effect through the formation of peroxynitrite with superoxide anion. The iNOS is mainly expressed in macrophages and is able to produce large amount of NO. The expression of iNOS is mainly regulated at the transcriptional level. The iNOS-mediated NO production plays a role in the development of atherosclerosis. Ganoderma lucidum (G. lucidum, Linzhi or Reishi) is a traditional herbal medicine which is commonly used as health supplement. Several studies have demonstrated its effectiveness against cancer, immunological disorders and cardiovascular diseases. The objective of the present study was to investigate the effect of G. lucidum on iNOS-mediated NO production in macrophages. Human monocytic cell (THP-1) derived macrophages were incubated with lipopolysaccharide (LPS) for 24 h. Such treatment significantly stimulated NO production (253% versus the control). Such a stimulatory effect was resulted from increased iNOS mRNA expression (270% versus the control) and iNOS activity (169.5% versus the control) in macrophages. The superoxide anion level was also elevated (150% versus the control) in LPS-treated macrophages. Treatment of macrophages with G. lucidum extract (100 μg/ml) completely abolished LPS-induced iNOS mRNA expression and NO production. Such an inhibitory effect of G. lucidum was mediated via its antioxidant action against LPS-induced superoxide anion generation in macrophages. These results suggest that G. lucidum may exert a therapeutic effect against atherosclerosis via ameliorating iNOS-mediated NO overproduction in macrophages. © Springer Science + Business Media, Inc. 2005.
Persistent Identifierhttp://hdl.handle.net/10722/80292
ISSN
2015 Impact Factor: 2.613
2015 SCImago Journal Rankings: 1.019
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, CWHen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorSiow, YLen_HK
dc.contributor.authorChoy, PCen_HK
dc.contributor.authorWan, EWYen_HK
dc.contributor.authorLau, CSen_HK
dc.contributor.authorO, Ken_HK
dc.date.accessioned2010-09-06T08:04:41Z-
dc.date.available2010-09-06T08:04:41Z-
dc.date.issued2005en_HK
dc.identifier.citationMolecular And Cellular Biochemistry, 2005, v. 275 n. 1-2, p. 165-171en_HK
dc.identifier.issn0300-8177en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80292-
dc.description.abstractNitric oxide (NO) is a principal mediator in many physiological and pathological processes. Overproduction of NO via the inducible nitric oxide synthase (iNOS) has cytotoxic effect through the formation of peroxynitrite with superoxide anion. The iNOS is mainly expressed in macrophages and is able to produce large amount of NO. The expression of iNOS is mainly regulated at the transcriptional level. The iNOS-mediated NO production plays a role in the development of atherosclerosis. Ganoderma lucidum (G. lucidum, Linzhi or Reishi) is a traditional herbal medicine which is commonly used as health supplement. Several studies have demonstrated its effectiveness against cancer, immunological disorders and cardiovascular diseases. The objective of the present study was to investigate the effect of G. lucidum on iNOS-mediated NO production in macrophages. Human monocytic cell (THP-1) derived macrophages were incubated with lipopolysaccharide (LPS) for 24 h. Such treatment significantly stimulated NO production (253% versus the control). Such a stimulatory effect was resulted from increased iNOS mRNA expression (270% versus the control) and iNOS activity (169.5% versus the control) in macrophages. The superoxide anion level was also elevated (150% versus the control) in LPS-treated macrophages. Treatment of macrophages with G. lucidum extract (100 μg/ml) completely abolished LPS-induced iNOS mRNA expression and NO production. Such an inhibitory effect of G. lucidum was mediated via its antioxidant action against LPS-induced superoxide anion generation in macrophages. These results suggest that G. lucidum may exert a therapeutic effect against atherosclerosis via ameliorating iNOS-mediated NO overproduction in macrophages. © Springer Science + Business Media, Inc. 2005.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177en_HK
dc.relation.ispartofMolecular and Cellular Biochemistryen_HK
dc.subjectHerbal medicineen_HK
dc.subjectInducible nitric oxide synthaseen_HK
dc.subjectNitric oxideen_HK
dc.subjectOxidative stressen_HK
dc.subject.meshAntioxidants - pharmacologyen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Culture Techniquesen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDrugs, Chinese Herbal - pharmacologyen_HK
dc.subject.meshGene Expression - drug effectsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMacrophages - drug effects - enzymologyen_HK
dc.subject.meshNitrates - analysis - metabolismen_HK
dc.subject.meshNitric Oxide Synthase Type II - antagonists & inhibitors - geneticsen_HK
dc.subject.meshNitrites - analysis - metabolismen_HK
dc.subject.meshPlant Extracts - isolation & purification - pharmacologyen_HK
dc.subject.meshRNA, Messenger - analysis - metabolismen_HK
dc.subject.meshReishi - chemistryen_HK
dc.subject.meshSuperoxides - analysis - metabolismen_HK
dc.subject.meshTetradecanoylphorbol Acetate - pharmacologyen_HK
dc.titleGanoderma lucidum inhibits inducible nitric oxide synthase expression in macrophagesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-8177&volume=275&spage=165&epage=171, 2005&date=2005&atitle=Ganoderma+lucidum+inhibits+inducible+nitric+oxide+synthase+expression+in+macrophagesen_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailLau, CS: cslau@hku.hken_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityLau, CS=rp01348en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11010-005-1352-9en_HK
dc.identifier.pmid16335796-
dc.identifier.scopuseid_2-s2.0-22044443080en_HK
dc.identifier.hkuros103573en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-22044443080&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume275en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage165en_HK
dc.identifier.epage171en_HK
dc.identifier.isiWOS:000230566400017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, CWH=23471396000en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK
dc.identifier.scopusauthoridSiow, YL=7003336463en_HK
dc.identifier.scopusauthoridChoy, PC=7006633002en_HK
dc.identifier.scopusauthoridWan, EWY=8685370300en_HK
dc.identifier.scopusauthoridLau, CS=14035682100en_HK
dc.identifier.scopusauthoridO, K=7006328603en_HK
dc.identifier.citeulike259824-

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