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Article: Expression and activities of three inducible enzymes in the healing of gastric ulcers in rats

TitleExpression and activities of three inducible enzymes in the healing of gastric ulcers in rats
Authors
Issue Date2003
PublisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htm
Citation
World Journal Of Gastroenterology, 2003, v. 9 n. 8, p. 1767-1771 How to Cite?
AbstractAim: To explore the roles of nitric oxide synthase (NOS), heme oxygenase (HO) and cyclooxygenase (COX) in gastric ulceration and to investigate the relationships of the expression and activities of these enzymes at different stages of gastric ulceration. Methods: Gastric ulcers (kissing ulcers) were induced by luminal application of acetic acid. Gastric tissue samples were obtained from the ulcer base, ulcer margin, and non-ulcerated area around the ulcer margin at different time intervals after ulcer induction. The mRNA expression and protein levels of inducible and constitutive isoforms of NOS, HO and COX were analyzed with RT-PCR and Western blotting methods. The activities of the total NOS, inducible NOS (iNOS), HO, and COX were also determined. Results: Differential expression of inducible iNOS, HO-1 and COX-2 and enzyme activities of NOS, HO and COX were found in the gastric ulcer base. High iNOS expression and activity were observed on day 1 to day 3 in severely inflamed ulcer tissues. Maximum expressions of HO-1 and COX-2 and enzyme activities of HO and COX lagged behind that of iNOS, and remained at high levels during the healing phase. Conclusion: The expression and activities of inducible NOS, HO-1 and COX-2 are found to be correlated to different stages of gastric ulceration. Inducible NOS may contribute to ulcer formation while HO-1 and COX-2 may promote ulcer healing.
Persistent Identifierhttp://hdl.handle.net/10722/80289
ISSN
2015 Impact Factor: 2.787
2015 SCImago Journal Rankings: 1.076
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, JSen_HK
dc.contributor.authorCho, CHen_HK
dc.contributor.authorWang, WPen_HK
dc.contributor.authorShen, XZen_HK
dc.contributor.authorCheng, CLen_HK
dc.contributor.authorKoo, MWLen_HK
dc.date.accessioned2010-09-06T08:04:39Z-
dc.date.available2010-09-06T08:04:39Z-
dc.date.issued2003en_HK
dc.identifier.citationWorld Journal Of Gastroenterology, 2003, v. 9 n. 8, p. 1767-1771en_HK
dc.identifier.issn1007-9327en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80289-
dc.description.abstractAim: To explore the roles of nitric oxide synthase (NOS), heme oxygenase (HO) and cyclooxygenase (COX) in gastric ulceration and to investigate the relationships of the expression and activities of these enzymes at different stages of gastric ulceration. Methods: Gastric ulcers (kissing ulcers) were induced by luminal application of acetic acid. Gastric tissue samples were obtained from the ulcer base, ulcer margin, and non-ulcerated area around the ulcer margin at different time intervals after ulcer induction. The mRNA expression and protein levels of inducible and constitutive isoforms of NOS, HO and COX were analyzed with RT-PCR and Western blotting methods. The activities of the total NOS, inducible NOS (iNOS), HO, and COX were also determined. Results: Differential expression of inducible iNOS, HO-1 and COX-2 and enzyme activities of NOS, HO and COX were found in the gastric ulcer base. High iNOS expression and activity were observed on day 1 to day 3 in severely inflamed ulcer tissues. Maximum expressions of HO-1 and COX-2 and enzyme activities of HO and COX lagged behind that of iNOS, and remained at high levels during the healing phase. Conclusion: The expression and activities of inducible NOS, HO-1 and COX-2 are found to be correlated to different stages of gastric ulceration. Inducible NOS may contribute to ulcer formation while HO-1 and COX-2 may promote ulcer healing.en_HK
dc.languageengen_HK
dc.publisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htmen_HK
dc.relation.ispartofWorld Journal of Gastroenterologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleExpression and activities of three inducible enzymes in the healing of gastric ulcers in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1007-9327&volume=9&issue=8&spage=1767&epage=1771&date=2003&atitle=Expression+and+activities+of+three+inducible+enzymes+in+the+healing+of+gastric+ulcers+in+ratsen_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3748/wjg.v9.i8.1767-
dc.identifier.pmid12918117-
dc.identifier.scopuseid_2-s2.0-0042428717en_HK
dc.identifier.hkuros86041en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042428717&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1767en_HK
dc.identifier.epage1771en_HK
dc.identifier.isiWOS:000184963600024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGuo, JS=7404488815en_HK
dc.identifier.scopusauthoridCho, CH=7403100461en_HK
dc.identifier.scopusauthoridWang, WP=7501765704en_HK
dc.identifier.scopusauthoridShen, XZ=7402721067en_HK
dc.identifier.scopusauthoridCheng, CL=7404797794en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK

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