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Article: A mechanistic study of proliferation induced by Angelica sinensis in a normal gastric epithelial cell line

TitleA mechanistic study of proliferation induced by Angelica sinensis in a normal gastric epithelial cell line
Authors
KeywordsAngelica sinensis
C-Myc
Gastric epithelial cells
ODC
Polysaccharide
Proliferation
Issue Date2001
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/biochempharm
Citation
Biochemical Pharmacology, 2001, v. 61 n. 11, p. 1439-1448 How to Cite?
AbstractIt has been reported that an extract from Angelica sinensis mainly consisting of polysaccharides (95%) prevented ethanol- or indomethacin-induced gastric mucosal damage (Cho CH et al. Planta Med 2000;66:348-51). However, it is not known whether Angelica sinensis has a direct stimulatory effect on the healing of gastric mucosal lesions. To study the hypothesis that Angelica sinensis has a direct mucosal healing effect in rats and in isolated gastric epithelial cells, we assessed the wound repair in both animals and normal cell culture (RGM-1), as well as [ 3H]thymidine incorporation, ornithine decarboxylase (ODC) activity, and ODC protein and c-Myc protein expression after different treatments in RGM-1 cells. We found that Angelica sinensis crude extract (ASCE) dose-dependently enhanced gastric ulcer healing in rats and promoted wound repair in RGM-1 cells. It also significantly stimulated [ 3H]thymidine incorporation and ODC activity in RGM-1 cells in a concentration-dependent manner. ODC and c-Myc protein expression was also increased as a result of this process. DL-α-difluoromethyl-ornithine repressed the [ 3H]thymidine incorporation and ODC activity induced by ASCE. Pretreatment with c-Myc antisense oligodeoxynucleotides blocked the stimulatory action of ASCE on [ 3H]thymidine incorporation and ODC protein expression. These data suggest that ASCE has a direct mucosal healing effect on gastric epithelial cells, while ODC and c-Myc are closely associated with this effect. © 2001 Elsevier Science Inc.
Persistent Identifierhttp://hdl.handle.net/10722/80284
ISSN
2015 Impact Factor: 5.091
2015 SCImago Journal Rankings: 2.263
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYe, YNen_HK
dc.contributor.authorLiu, ESLen_HK
dc.contributor.authorShin, VYen_HK
dc.contributor.authorKoo, MWLen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorWei, EQen_HK
dc.contributor.authorMatsui, Hen_HK
dc.contributor.authorCho, CHen_HK
dc.date.accessioned2010-09-06T08:04:36Z-
dc.date.available2010-09-06T08:04:36Z-
dc.date.issued2001en_HK
dc.identifier.citationBiochemical Pharmacology, 2001, v. 61 n. 11, p. 1439-1448en_HK
dc.identifier.issn0006-2952en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80284-
dc.description.abstractIt has been reported that an extract from Angelica sinensis mainly consisting of polysaccharides (95%) prevented ethanol- or indomethacin-induced gastric mucosal damage (Cho CH et al. Planta Med 2000;66:348-51). However, it is not known whether Angelica sinensis has a direct stimulatory effect on the healing of gastric mucosal lesions. To study the hypothesis that Angelica sinensis has a direct mucosal healing effect in rats and in isolated gastric epithelial cells, we assessed the wound repair in both animals and normal cell culture (RGM-1), as well as [ 3H]thymidine incorporation, ornithine decarboxylase (ODC) activity, and ODC protein and c-Myc protein expression after different treatments in RGM-1 cells. We found that Angelica sinensis crude extract (ASCE) dose-dependently enhanced gastric ulcer healing in rats and promoted wound repair in RGM-1 cells. It also significantly stimulated [ 3H]thymidine incorporation and ODC activity in RGM-1 cells in a concentration-dependent manner. ODC and c-Myc protein expression was also increased as a result of this process. DL-α-difluoromethyl-ornithine repressed the [ 3H]thymidine incorporation and ODC activity induced by ASCE. Pretreatment with c-Myc antisense oligodeoxynucleotides blocked the stimulatory action of ASCE on [ 3H]thymidine incorporation and ODC protein expression. These data suggest that ASCE has a direct mucosal healing effect on gastric epithelial cells, while ODC and c-Myc are closely associated with this effect. © 2001 Elsevier Science Inc.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/biochempharmen_HK
dc.relation.ispartofBiochemical Pharmacologyen_HK
dc.rightsBiochemical Pharmacology. Copyright © Elsevier Inc.en_HK
dc.subjectAngelica sinensisen_HK
dc.subjectC-Mycen_HK
dc.subjectGastric epithelial cellsen_HK
dc.subjectODCen_HK
dc.subjectPolysaccharideen_HK
dc.subjectProliferationen_HK
dc.titleA mechanistic study of proliferation induced by Angelica sinensis in a normal gastric epithelial cell lineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-2952&volume=61&spage=1439&epage=1448&date=2001&atitle=A+mechanistic+study+of+proliferation+induced+by+Angelica+sinensis+in+a+normal+gastric+epithelial+cell+lineen_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-2952(01)00625-6en_HK
dc.identifier.pmid11331080-
dc.identifier.scopuseid_2-s2.0-0035371005en_HK
dc.identifier.hkuros58930en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035371005&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume61en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1439en_HK
dc.identifier.epage1448en_HK
dc.identifier.isiWOS:000168331700013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYe, YN=7401627402en_HK
dc.identifier.scopusauthoridLiu, ESL=7202240071en_HK
dc.identifier.scopusauthoridShin, VY=7003491170en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK
dc.identifier.scopusauthoridLi, Y=54970757000en_HK
dc.identifier.scopusauthoridWei, EQ=7102156300en_HK
dc.identifier.scopusauthoridMatsui, H=7401527136en_HK
dc.identifier.scopusauthoridCho, CH=7403100461en_HK

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