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Article: Openers of calcium-activated potassium channels and endothelium-dependent hyperpolarizations in the guinea pig carotid artery

TitleOpeners of calcium-activated potassium channels and endothelium-dependent hyperpolarizations in the guinea pig carotid artery
Authors
Keywords1-EBIO
Ca2+-activated potassium channel
EDHF
Endothelium
NS-309
Riluzole
Issue Date2008
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00210/index.htm
Citation
Naunyn-Schmiedeberg's Archives Of Pharmacology, 2008, v. 377 n. 2, p. 101-109 How to Cite?
AbstractThis study was designed to determine whether putative openers of calcium-activated potassium channels of small and/or intermediate conductance (SKCa and IKCa) induce vascular smooth muscle hyperpolarizations and to identify the underlying mechanisms. The membrane potential of guinea pig carotid artery smooth muscle cells was recorded with intracellular microelectrodes in the presence of N ω-nitro-l- arginine and indomethacin. Acetylcholine and NS-309 produced endothelium-dependent hyperpolarizations. The effects of acetylcholine were partially and significantly inhibited by apamin. The combinations of charybdotoxin plus apamin and TRAM-34 plus apamin markedly and significantly reduced these hyperpolarizations. 1-ethyl-2-benzimidazolinone (1-EBIO) induced hyperpolarizations that were unaffected by TRAM-34 but partially inhibited by charybdotoxin, apamin, TRAM-34 plus apamin, and charybdotoxin plus apamin. Riluzole produced only marginal hyperpolarizations. Therefore, in the guinea pig carotid artery, endothelium-dependent hyperpolarization to acetylcholine involves the activation of both SKCa and IKCa, with a predominant role for the former channel. 1-EBIO is a non-selective and weak opener of SKCa, while riluzole is virtually ineffective. By contrast, NS-309 is a reasonably potent and selective opener of both SKCa and IKCa, and this compound mimics the endothelium-dependent hyperpolarizations to acetylcholine. © 2008 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/80273
ISSN
2015 Impact Factor: 2.376
2015 SCImago Journal Rankings: 0.859
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeuranguer, Ven_HK
dc.contributor.authorGluais, Pen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.contributor.authorVerbeuren, TJen_HK
dc.contributor.authorFélétou, Men_HK
dc.date.accessioned2010-09-06T08:04:28Z-
dc.date.available2010-09-06T08:04:28Z-
dc.date.issued2008en_HK
dc.identifier.citationNaunyn-Schmiedeberg's Archives Of Pharmacology, 2008, v. 377 n. 2, p. 101-109en_HK
dc.identifier.issn0028-1298en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80273-
dc.description.abstractThis study was designed to determine whether putative openers of calcium-activated potassium channels of small and/or intermediate conductance (SKCa and IKCa) induce vascular smooth muscle hyperpolarizations and to identify the underlying mechanisms. The membrane potential of guinea pig carotid artery smooth muscle cells was recorded with intracellular microelectrodes in the presence of N ω-nitro-l- arginine and indomethacin. Acetylcholine and NS-309 produced endothelium-dependent hyperpolarizations. The effects of acetylcholine were partially and significantly inhibited by apamin. The combinations of charybdotoxin plus apamin and TRAM-34 plus apamin markedly and significantly reduced these hyperpolarizations. 1-ethyl-2-benzimidazolinone (1-EBIO) induced hyperpolarizations that were unaffected by TRAM-34 but partially inhibited by charybdotoxin, apamin, TRAM-34 plus apamin, and charybdotoxin plus apamin. Riluzole produced only marginal hyperpolarizations. Therefore, in the guinea pig carotid artery, endothelium-dependent hyperpolarization to acetylcholine involves the activation of both SKCa and IKCa, with a predominant role for the former channel. 1-EBIO is a non-selective and weak opener of SKCa, while riluzole is virtually ineffective. By contrast, NS-309 is a reasonably potent and selective opener of both SKCa and IKCa, and this compound mimics the endothelium-dependent hyperpolarizations to acetylcholine. © 2008 Springer-Verlag.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00210/index.htmen_HK
dc.relation.ispartofNaunyn-Schmiedeberg's Archives of Pharmacologyen_HK
dc.subject1-EBIOen_HK
dc.subjectCa2+-activated potassium channelen_HK
dc.subjectEDHFen_HK
dc.subjectEndotheliumen_HK
dc.subjectNS-309en_HK
dc.subjectRiluzoleen_HK
dc.titleOpeners of calcium-activated potassium channels and endothelium-dependent hyperpolarizations in the guinea pig carotid arteryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0028-1298&volume=377&spage=101&epage=109&date=2008&atitle=Openers+of+calcium-activated+potassium+channels+and+endothelium-dependent+hyperpolarizations+in+the+guinea+pig+carotid+arteryen_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00210-008-0267-xen_HK
dc.identifier.pmid18264694-
dc.identifier.scopuseid_2-s2.0-41049097099en_HK
dc.identifier.hkuros151962en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-41049097099&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume377en_HK
dc.identifier.issue2en_HK
dc.identifier.spage101en_HK
dc.identifier.epage109en_HK
dc.identifier.isiWOS:000254204800001-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLeuranguer, V=6508390373en_HK
dc.identifier.scopusauthoridGluais, P=6602456462en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK
dc.identifier.scopusauthoridVerbeuren, TJ=7007006534en_HK
dc.identifier.scopusauthoridFélétou, M=7006461826en_HK
dc.identifier.citeulike2772715-

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