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Article: Cigarette smoke extracts delay wound healing in the stomach: Involvement of polyamine synthesis

TitleCigarette smoke extracts delay wound healing in the stomach: Involvement of polyamine synthesis
Authors
Issue Date2002
PublisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/
Citation
Experimental Biology And Medicine, 2002, v. 227 n. 2, p. 114-124 How to Cite?
AbstractThe association between cigarette smoking and peptic ulcer diseases has been well established. Ornithine decarboxylase (ODC) is crucial for the gastroprotective and mucosal growth promoting effects in gastric ulcer healing. The aim of this study is to elucidate the possible mechanism of how inhibition of ODC activity is involved in the delay of ulcer healing, if any, by cigarette smoke extracts (CSE). CSE were fractionated into chloroform extract (CE) and ethanol extract (EE). In in vivo study, rats with acetic acid-induced ulcers were given CE or EE intragastrically (2.5 or 5 mg/kg) once daily for 3 days. Ulcer sizes were significantly larger after CE or EE administration, followed by an increase in myeloperoxidase activity and a reduction in cell proliferation. However, both CSE stimulated the number of microvessels following the increase of basic fibroblast growth factor. In in vitro studies, the effect of CE or EE (10, 40, or 100 μg/ml) on cell migration and cell proliferation were measured using an in vitro wound model and [3H]-thymidine incorporation assay, respectively. Both CSE delayed cell migration and decreased cell proliferation, which were accompanied with a reduction in ODC activity. Exogenous spermidine (5 or 10 μM) could reverse the inhibitory action on cell proliferation and ODC activity induced by CSE. In conclusion, both CSE significantly delayed ulcer healing as a result of reduction in cell proliferation and cell migration. All these effects are, in part, related to the reduction of polyamine synthesis.
Persistent Identifierhttp://hdl.handle.net/10722/80268
ISSN
2002 Impact Factor: 2.714
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShin, VYen_HK
dc.contributor.authorLiu, ESLen_HK
dc.contributor.authorKoo, MWLen_HK
dc.contributor.authorWang, JYen_HK
dc.contributor.authorMatsui, Hen_HK
dc.contributor.authorCho, CHen_HK
dc.date.accessioned2010-09-06T08:04:25Z-
dc.date.available2010-09-06T08:04:25Z-
dc.date.issued2002en_HK
dc.identifier.citationExperimental Biology And Medicine, 2002, v. 227 n. 2, p. 114-124en_HK
dc.identifier.issn0037-9727en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80268-
dc.description.abstractThe association between cigarette smoking and peptic ulcer diseases has been well established. Ornithine decarboxylase (ODC) is crucial for the gastroprotective and mucosal growth promoting effects in gastric ulcer healing. The aim of this study is to elucidate the possible mechanism of how inhibition of ODC activity is involved in the delay of ulcer healing, if any, by cigarette smoke extracts (CSE). CSE were fractionated into chloroform extract (CE) and ethanol extract (EE). In in vivo study, rats with acetic acid-induced ulcers were given CE or EE intragastrically (2.5 or 5 mg/kg) once daily for 3 days. Ulcer sizes were significantly larger after CE or EE administration, followed by an increase in myeloperoxidase activity and a reduction in cell proliferation. However, both CSE stimulated the number of microvessels following the increase of basic fibroblast growth factor. In in vitro studies, the effect of CE or EE (10, 40, or 100 μg/ml) on cell migration and cell proliferation were measured using an in vitro wound model and [3H]-thymidine incorporation assay, respectively. Both CSE delayed cell migration and decreased cell proliferation, which were accompanied with a reduction in ODC activity. Exogenous spermidine (5 or 10 μM) could reverse the inhibitory action on cell proliferation and ODC activity induced by CSE. In conclusion, both CSE significantly delayed ulcer healing as a result of reduction in cell proliferation and cell migration. All these effects are, in part, related to the reduction of polyamine synthesis.en_HK
dc.languageengen_HK
dc.publisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/en_HK
dc.relation.ispartofExperimental Biology and Medicineen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshGastric Mucosa - metabolism - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshPolyamines - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshSmoking - adverse effects - metabolism - physiopathologyen_HK
dc.subject.meshStomach Ulcer - etiology - metabolism - physiopathologyen_HK
dc.subject.meshWound Healingen_HK
dc.titleCigarette smoke extracts delay wound healing in the stomach: Involvement of polyamine synthesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1535-3702&volume=227 &issue=2&spage=114&epage=124&date=2002&atitle=Cigarette+smoke+extracts+delay+wound+healing+in+the+stomach:+Involvement+of+polyamine+synthesisen_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11815675-
dc.identifier.scopuseid_2-s2.0-0036486325en_HK
dc.identifier.hkuros76683en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036486325&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume227en_HK
dc.identifier.issue2en_HK
dc.identifier.spage114en_HK
dc.identifier.epage124en_HK
dc.identifier.isiWOS:000173436500006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShin, VY=7003491170en_HK
dc.identifier.scopusauthoridLiu, ESL=7202240071en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK
dc.identifier.scopusauthoridWang, JY=8438337300en_HK
dc.identifier.scopusauthoridMatsui, H=7401527136en_HK
dc.identifier.scopusauthoridCho, CH=14067000400en_HK

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