File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Cholestin inhibits cholesterol synthesis and secretion in hepatic cells (HepG2)

TitleCholestin inhibits cholesterol synthesis and secretion in hepatic cells (HepG2)
Authors
KeywordsCholesterol
HMG-CoA reductase
Monascus purpureus
Red yeast rice
Statin
Issue Date2002
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177
Citation
Molecular And Cellular Biochemistry, 2002, v. 233 n. 1-2, p. 153-158 How to Cite?
AbstractHyperlipidemia is a well-known risk factor for atherosclerosis and statins are widely used to treat patients with elevated levels of lipids in their plasma. Notwithstanding the proven benefits of statin drugs on both primary and secondary prevention of heart disease, the high cost of statin treatment, in addition to possible side effects such as liver function abnormalities, may limit their widespread use. We conducted a study on a natural product as an alternative to statin treatment. Cholestin, a dietary supplement, is prepared from rice fermented with red yeast (Monascus purpureus), which has been shown to significantly decrease total cholesterol levels in hyperlipidemic subjects. Our objective was to determine the cellular effect of Cholestin on cholesterol synthesis in human hepatic cells (HepG2) and the mechanism by which it caused a change in lipid metabolism. Cholestin had a direct inhibitory effect on HMG-CoA reductase activity (78-69% of control). Cholesterol levels in HepG2 cells treated with Cholestin (25-100 μg/mL) were significantly reduced in a dose-dependent manner (81-45% of control, respectively). This reduction was associated with decreased synthesis and secretion of both unesterified cholesterol (54-31 and 33-14% of control, respectively) and cholesteryl ester (18-6 and 37-19% of control, respectively). These results indicate that one of the anti-hyperlipidemic actions of Cholestin is a consequence of an inhibitory effect on cholesterol biosynthesis in hepatic cells and provide the first documentation of a biomolecular action of red yeast rice.
Persistent Identifierhttp://hdl.handle.net/10722/80266
ISSN
2015 Impact Factor: 2.613
2015 SCImago Journal Rankings: 1.019
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorLynn, EGen_HK
dc.contributor.authorCheung, Fen_HK
dc.contributor.authorTsang, PSYen_HK
dc.contributor.authorO, Ken_HK
dc.date.accessioned2010-09-06T08:04:23Z-
dc.date.available2010-09-06T08:04:23Z-
dc.date.issued2002en_HK
dc.identifier.citationMolecular And Cellular Biochemistry, 2002, v. 233 n. 1-2, p. 153-158en_HK
dc.identifier.issn0300-8177en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80266-
dc.description.abstractHyperlipidemia is a well-known risk factor for atherosclerosis and statins are widely used to treat patients with elevated levels of lipids in their plasma. Notwithstanding the proven benefits of statin drugs on both primary and secondary prevention of heart disease, the high cost of statin treatment, in addition to possible side effects such as liver function abnormalities, may limit their widespread use. We conducted a study on a natural product as an alternative to statin treatment. Cholestin, a dietary supplement, is prepared from rice fermented with red yeast (Monascus purpureus), which has been shown to significantly decrease total cholesterol levels in hyperlipidemic subjects. Our objective was to determine the cellular effect of Cholestin on cholesterol synthesis in human hepatic cells (HepG2) and the mechanism by which it caused a change in lipid metabolism. Cholestin had a direct inhibitory effect on HMG-CoA reductase activity (78-69% of control). Cholesterol levels in HepG2 cells treated with Cholestin (25-100 μg/mL) were significantly reduced in a dose-dependent manner (81-45% of control, respectively). This reduction was associated with decreased synthesis and secretion of both unesterified cholesterol (54-31 and 33-14% of control, respectively) and cholesteryl ester (18-6 and 37-19% of control, respectively). These results indicate that one of the anti-hyperlipidemic actions of Cholestin is a consequence of an inhibitory effect on cholesterol biosynthesis in hepatic cells and provide the first documentation of a biomolecular action of red yeast rice.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177en_HK
dc.relation.ispartofMolecular and Cellular Biochemistryen_HK
dc.subjectCholesterolen_HK
dc.subjectHMG-CoA reductaseen_HK
dc.subjectMonascus purpureusen_HK
dc.subjectRed yeast riceen_HK
dc.subjectStatinen_HK
dc.titleCholestin inhibits cholesterol synthesis and secretion in hepatic cells (HepG2)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-8177&volume=233&spage=153&epage=158&date=2002&atitle=Cholestin+inhibits+cholesterol+synthesis+and+secretion+in+hepatic+cells+(HepG2)en_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1017487815091en_HK
dc.identifier.pmid12083370-
dc.identifier.scopuseid_2-s2.0-0036308901en_HK
dc.identifier.hkuros73449en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036308901&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume233en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage153en_HK
dc.identifier.epage158en_HK
dc.identifier.isiWOS:000175635300020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK
dc.identifier.scopusauthoridLynn, EG=7004176471en_HK
dc.identifier.scopusauthoridCheung, F=35073678200en_HK
dc.identifier.scopusauthoridTsang, PSY=7102404071en_HK
dc.identifier.scopusauthoridO, K=7006328603en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats