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Article: Expression and activity patterns of iNOS and eNOS in acetic acid induced gastric ulcers in rats

TitleExpression and activity patterns of iNOS and eNOS in acetic acid induced gastric ulcers in rats
Authors
KeywordsDisease models, animal
Nitric oxide synthase/biosynthesis
Nitric oxide synthase/metabolism
Stomach ulcer/pathology
Issue Date2001
Publisher中國中西醫結合學會. The Journal's web site is located at http://xxhb.chinajournal.net.cn/
Citation
World Chinese Journal Of Digestology, 2001, v. 9 n. 3, p. 288-292 How to Cite?
AbstractAIM - To study the expression and activity patterns of constitutive and inducible forms of nitric oxide synthase (eNOS and iNOS) in gastric ulcer and their roles in gastric ulcer healing. METHODS - Rat models of gastric ulcer were induced by intraluminal application of acetic acid solution. Stomach samples from the base, margin and normal tissues around the gastric ulcers were collected at different times after ulcer induction. Expressions of eNOS and iNOS mRNA and their protein levels were detected by RT-PCR and Western Blot methods. NOS activities were measured as the ability of tissue homogenates to convert L-[3H]-arginine to L-[3H] citrulline. RESULTS - A high level of expression and enzyme activity of iNOS were detected in the ulcer base in day 1 to day 3 after ulcer induction when the tissue inflammation was most severe. The protein level and activity of iNOS in normal gastric tissue or the ulcerated tissue in the healing phase were undetectable or at a low level. The expression and activity of eNOS was relatively stable during the whole experimental period. CONCLUSION - There are restricted induction and regulation of iNOS expression and activity in the healing process of gastric ulcer. Nitric oxide mainly derived from the activity of iNOS, plays an important role in the pathological process of inflammation and gastric ulcer healing.
Persistent Identifierhttp://hdl.handle.net/10722/80263
ISSN
2015 SCImago Journal Rankings: 0.104
References

 

DC FieldValueLanguage
dc.contributor.authorJin Sheng Guoen_HK
dc.contributor.authorKoo, MWLen_HK
dc.contributor.authorJi Yao Wangen_HK
dc.contributor.authorChi Hin Choen_HK
dc.date.accessioned2010-09-06T08:04:21Z-
dc.date.available2010-09-06T08:04:21Z-
dc.date.issued2001en_HK
dc.identifier.citationWorld Chinese Journal Of Digestology, 2001, v. 9 n. 3, p. 288-292en_HK
dc.identifier.issn1009-3079en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80263-
dc.description.abstractAIM - To study the expression and activity patterns of constitutive and inducible forms of nitric oxide synthase (eNOS and iNOS) in gastric ulcer and their roles in gastric ulcer healing. METHODS - Rat models of gastric ulcer were induced by intraluminal application of acetic acid solution. Stomach samples from the base, margin and normal tissues around the gastric ulcers were collected at different times after ulcer induction. Expressions of eNOS and iNOS mRNA and their protein levels were detected by RT-PCR and Western Blot methods. NOS activities were measured as the ability of tissue homogenates to convert L-[3H]-arginine to L-[3H] citrulline. RESULTS - A high level of expression and enzyme activity of iNOS were detected in the ulcer base in day 1 to day 3 after ulcer induction when the tissue inflammation was most severe. The protein level and activity of iNOS in normal gastric tissue or the ulcerated tissue in the healing phase were undetectable or at a low level. The expression and activity of eNOS was relatively stable during the whole experimental period. CONCLUSION - There are restricted induction and regulation of iNOS expression and activity in the healing process of gastric ulcer. Nitric oxide mainly derived from the activity of iNOS, plays an important role in the pathological process of inflammation and gastric ulcer healing.en_HK
dc.languageengen_HK
dc.publisher中國中西醫結合學會. The Journal's web site is located at http://xxhb.chinajournal.net.cn/en_HK
dc.relation.ispartofWorld Chinese Journal of Digestologyen_HK
dc.subjectDisease models, animalen_HK
dc.subjectNitric oxide synthase/biosynthesisen_HK
dc.subjectNitric oxide synthase/metabolismen_HK
dc.subjectStomach ulcer/pathologyen_HK
dc.titleExpression and activity patterns of iNOS and eNOS in acetic acid induced gastric ulcers in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1009-3079&volume=9 &issue=3&spage=&epage=&date=2001&atitle=Expression+and+activity+patterns+of+iNOS+and+eNOS+in+acetic+acid+induced+gastric+ulcers+in+ratsen_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0035075769en_HK
dc.identifier.hkuros61369en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035075769&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue3en_HK
dc.identifier.spage288en_HK
dc.identifier.epage292en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridJin Sheng Guo=7409653183en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK
dc.identifier.scopusauthoridJi Yao Wang=7409832905en_HK
dc.identifier.scopusauthoridChi Hin Cho=7409803382en_HK

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