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Article: Status of the Endothelium-derived Hyperpolarizing Factor Pathway in Coronary Arteries After Heterotopic Heart Transplantation

TitleStatus of the Endothelium-derived Hyperpolarizing Factor Pathway in Coronary Arteries After Heterotopic Heart Transplantation
Authors
Issue Date2007
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/healun
Citation
Journal Of Heart And Lung Transplantation, 2007, v. 26 n. 1, p. 48-55 How to Cite?
AbstractBackground: After the first year of transplantation, the major limitation to long-term survival is the development of graft coronary vasculopathy, characterized by a pathologic activation of the endothelium with an attendant loss of its regulatory properties on homeostasis of the vascular wall. The present study was designed to evaluate the integrity of coronary vascular relaxations attributed to the endothelium-derived hyperpolarizing factor (EDHF) and to study hyperpolarization of smooth muscle cells after heterotopic heart transplantation. Methods: Six weeks after heart transplantation in a porcine model, vascular reactivity studies of control, native and allograft epicardial coronary artery rings were performed in standard organ chamber experiments. Moreover, membrane potential measurements were made with intracellular microelectrodes in rings of native and allograft coronary arteries. Results: There was a significant decrease in endothelium-dependent relaxations to 5-hydroxytryptamine (5-HT), high doses of bradykinin (BK) alone and BK plus N-ω-nitro-L-arginine (L-NNA) in rings from allograft compared to native, whereas the variation was significantly increased in response to cromakalim, a K +-ATP channel opener. Electrical and mechanical recordings showed no alteration in the resting membrane potential of smooth muscle cells, depolarization during contraction to prostaglandin F 2α (PGF 2α), or hyperpolarization in the presence of BK + L-NNA in rings of allograft vs native. Conclusions: In this swine model of heart transplantation, part of the reduction in endothelium-dependent relaxations to BK may be attributed to an alteration in the activity of EDHF. This impairment of EDHF-mediated relaxations may compound the endothelial dysfunction preceding the development of coronary graft vasculopathy. © 2007 International Society for Heart and Lung Transplantation.
Persistent Identifierhttp://hdl.handle.net/10722/80227
ISSN
2015 Impact Factor: 7.509
2015 SCImago Journal Rankings: 3.655
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPerrault, LPen_HK
dc.contributor.authorAubin, MCen_HK
dc.contributor.authorMalo, Oen_HK
dc.contributor.authorThollon, Cen_HK
dc.contributor.authorVilleneuve, Nen_HK
dc.contributor.authorVilaine, JPen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-06T08:03:57Z-
dc.date.available2010-09-06T08:03:57Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Heart And Lung Transplantation, 2007, v. 26 n. 1, p. 48-55en_HK
dc.identifier.issn1053-2498en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80227-
dc.description.abstractBackground: After the first year of transplantation, the major limitation to long-term survival is the development of graft coronary vasculopathy, characterized by a pathologic activation of the endothelium with an attendant loss of its regulatory properties on homeostasis of the vascular wall. The present study was designed to evaluate the integrity of coronary vascular relaxations attributed to the endothelium-derived hyperpolarizing factor (EDHF) and to study hyperpolarization of smooth muscle cells after heterotopic heart transplantation. Methods: Six weeks after heart transplantation in a porcine model, vascular reactivity studies of control, native and allograft epicardial coronary artery rings were performed in standard organ chamber experiments. Moreover, membrane potential measurements were made with intracellular microelectrodes in rings of native and allograft coronary arteries. Results: There was a significant decrease in endothelium-dependent relaxations to 5-hydroxytryptamine (5-HT), high doses of bradykinin (BK) alone and BK plus N-ω-nitro-L-arginine (L-NNA) in rings from allograft compared to native, whereas the variation was significantly increased in response to cromakalim, a K +-ATP channel opener. Electrical and mechanical recordings showed no alteration in the resting membrane potential of smooth muscle cells, depolarization during contraction to prostaglandin F 2α (PGF 2α), or hyperpolarization in the presence of BK + L-NNA in rings of allograft vs native. Conclusions: In this swine model of heart transplantation, part of the reduction in endothelium-dependent relaxations to BK may be attributed to an alteration in the activity of EDHF. This impairment of EDHF-mediated relaxations may compound the endothelial dysfunction preceding the development of coronary graft vasculopathy. © 2007 International Society for Heart and Lung Transplantation.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/healunen_HK
dc.relation.ispartofJournal of Heart and Lung Transplantationen_HK
dc.rightsThe Journal of Heart and Lung Transplantation. Copyright © Elsevier Inc.en_HK
dc.titleStatus of the Endothelium-derived Hyperpolarizing Factor Pathway in Coronary Arteries After Heterotopic Heart Transplantationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1053-2498&volume=26:1&spage=48&epage=55&date=2006&atitle=Status+of+the+endothelium-derived+hyperpolarizing+factor+pathway+in+coronary+arteries+after+heterotopic+heart+transplantationen_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.healun.2006.10.015en_HK
dc.identifier.pmid17234517en_HK
dc.identifier.scopuseid_2-s2.0-33846213630en_HK
dc.identifier.hkuros136339en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846213630&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue1en_HK
dc.identifier.spage48en_HK
dc.identifier.epage55en_HK
dc.identifier.isiWOS:000243950900008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPerrault, LP=7004370552en_HK
dc.identifier.scopusauthoridAubin, MC=8527346100en_HK
dc.identifier.scopusauthoridMalo, O=6602767845en_HK
dc.identifier.scopusauthoridThollon, C=6602540205en_HK
dc.identifier.scopusauthoridVilleneuve, N=7003458215en_HK
dc.identifier.scopusauthoridVilaine, JP=7004617134en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK

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