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Article: Cathelicidin stimulates colonic mucus synthesis by up-regulating MUC1 and MUC2 expression through a mitogen-activated protein kinase pathway

TitleCathelicidin stimulates colonic mucus synthesis by up-regulating MUC1 and MUC2 expression through a mitogen-activated protein kinase pathway
Authors
KeywordsCathelicidin
Mitogen-activated protein kinase
Mucin
Mucus
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal Of Cellular Biochemistry, 2008, v. 104 n. 1, p. 251-258 How to Cite?
AbstractMucus forms the physical barrier along the gastrointestinal tract. It plays an important role to prevent mucosal damage and inflammation. Our animal study showed that antibacterial peptide 'cathelicidin' increased mucus thickness and prevented inflammation in the colon. In the current study, we examined the direct effect and mechanisms by which the peptide increased mucus synthesis in a human colonic cell line (HT-29). Human cathelicidin (LL-37) dose-dependently (10-40 mg/ml) and significantly stimulated mucus synthesis by increasing the D-[6-3H] glucosamine incorporation in the cells. Real-time PCR data showed that addition of LL-37 induced more than 50% increase in MUC1 and MUC2 mRNA levels. Treatment with MUC1 and MUC2 siRNAs normalized the stimulatory action of LL-37 on mucus synthesis. LL-37 also activated the phosphorylation of mitogen-activated protein (MAP) kinase in the cells. A specific inhibitor of the MAP kinase pathway, U0126, completely blocked the increase of MUC1 and MUC2 expression as well as mucus synthesis by LL-37. Taken together, LL-37 can directly stimulate mucus synthesis through activation of MUC1 and MUC2 expression and MAP kinase pathway in human colonic cells. © 2007 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/80215
ISSN
2015 Impact Factor: 3.446
2015 SCImago Journal Rankings: 1.520
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilCUHK 7565/06M
Funding Information:

Grant sponsor: Hong Kong Research Grants Council; Grant number: CUHK 7565/06M.

References

 

DC FieldValueLanguage
dc.contributor.authorTai, EKKen_HK
dc.contributor.authorWong, HPSen_HK
dc.contributor.authorLam, EKYen_HK
dc.contributor.authorWu, WKKen_HK
dc.contributor.authorYu, Len_HK
dc.contributor.authorKoo, MWLen_HK
dc.contributor.authorCho, CHen_HK
dc.date.accessioned2010-09-06T08:03:48Z-
dc.date.available2010-09-06T08:03:48Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Cellular Biochemistry, 2008, v. 104 n. 1, p. 251-258en_HK
dc.identifier.issn0730-2312en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80215-
dc.description.abstractMucus forms the physical barrier along the gastrointestinal tract. It plays an important role to prevent mucosal damage and inflammation. Our animal study showed that antibacterial peptide 'cathelicidin' increased mucus thickness and prevented inflammation in the colon. In the current study, we examined the direct effect and mechanisms by which the peptide increased mucus synthesis in a human colonic cell line (HT-29). Human cathelicidin (LL-37) dose-dependently (10-40 mg/ml) and significantly stimulated mucus synthesis by increasing the D-[6-3H] glucosamine incorporation in the cells. Real-time PCR data showed that addition of LL-37 induced more than 50% increase in MUC1 and MUC2 mRNA levels. Treatment with MUC1 and MUC2 siRNAs normalized the stimulatory action of LL-37 on mucus synthesis. LL-37 also activated the phosphorylation of mitogen-activated protein (MAP) kinase in the cells. A specific inhibitor of the MAP kinase pathway, U0126, completely blocked the increase of MUC1 and MUC2 expression as well as mucus synthesis by LL-37. Taken together, LL-37 can directly stimulate mucus synthesis through activation of MUC1 and MUC2 expression and MAP kinase pathway in human colonic cells. © 2007 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503en_HK
dc.relation.ispartofJournal of Cellular Biochemistryen_HK
dc.rightsJournal of Cellular Biochemistry. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectCathelicidinen_HK
dc.subjectMitogen-activated protein kinaseen_HK
dc.subjectMucinen_HK
dc.subjectMucusen_HK
dc.titleCathelicidin stimulates colonic mucus synthesis by up-regulating MUC1 and MUC2 expression through a mitogen-activated protein kinase pathwayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0730-2312&volume=104&issue=1&spage=251&epage=258&date=2007&atitle=Cathelicidin+Stimulates+Colonic+Mucus+Synthesis+By+Up-regulating+Muc1+And+Muc2+Expression+Through+A+Mitogen-activated+Protein+Kinase+Pathwayen_HK
dc.identifier.emailWong, HPS: hpswong@hkusua.hku.hken_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.authorityWong, HPS=rp00808en_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jcb.21615en_HK
dc.identifier.pmid18059019en_HK
dc.identifier.scopuseid_2-s2.0-46449087690en_HK
dc.identifier.hkuros161847en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-46449087690&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume104en_HK
dc.identifier.issue1en_HK
dc.identifier.spage251en_HK
dc.identifier.epage258en_HK
dc.identifier.isiWOS:000260277000022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTai, EKK=9842278900en_HK
dc.identifier.scopusauthoridWong, HPS=8644138100en_HK
dc.identifier.scopusauthoridLam, EKY=8644138600en_HK
dc.identifier.scopusauthoridWu, WKK=18345422600en_HK
dc.identifier.scopusauthoridYu, L=16314581700en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK
dc.identifier.scopusauthoridCho, CH=14067000400en_HK

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