File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Low molecular weight G-proteins of rho-family mediate relaxations to bradykinin in porcine coronary arteries

TitleLow molecular weight G-proteins of rho-family mediate relaxations to bradykinin in porcine coronary arteries
Authors
KeywordsBradykinin
Endothelium
GTP-binding proteins
Nitric oxide
Issue Date2003
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html
Citation
Acta Pharmacologica Sinica, 2003, v. 24 n. 11, p. 1070-1076 How to Cite?
AbstractAIM: To determine whether or not low molecular G-proteins are involved in the endothelium-dependent relaxations to bradykinin. METHODS: The effects of botulinum ADP-ribosyltranferase C3 were studied in porcine coronary arteries and endothelial cells. RESULTS: Incubation of membrane fractions isolated from endothelial cells with the enzyme and 32P-NAD resulted in the ribosylation of the proteins with molecular weight of 24-25 kDa. Radio labelling of these proteins was suppressed in the presence of guanosine 5′-O-(3-thiotriphosphate) (GTP-γS), a hydrolysis-resistant analog of GTP. In the isolated arteries, ADP-ribosyltransferase C3 attenuated the relaxations to bradykinin during contractions with prostaglandin F 2α in the presence of tween 80 (non ionic detergent), but not in the absence of tween 80. CONCLUSION: Low molecular weight G-proteins of the Rho family contribute to the mechanism of relaxation induced by bradykinin.
Persistent Identifierhttp://hdl.handle.net/10722/80207
ISSN
2015 Impact Factor: 3.166
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShibano, Ten_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-06T08:03:43Z-
dc.date.available2010-09-06T08:03:43Z-
dc.date.issued2003en_HK
dc.identifier.citationActa Pharmacologica Sinica, 2003, v. 24 n. 11, p. 1070-1076en_HK
dc.identifier.issn1671-4083en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80207-
dc.description.abstractAIM: To determine whether or not low molecular G-proteins are involved in the endothelium-dependent relaxations to bradykinin. METHODS: The effects of botulinum ADP-ribosyltranferase C3 were studied in porcine coronary arteries and endothelial cells. RESULTS: Incubation of membrane fractions isolated from endothelial cells with the enzyme and 32P-NAD resulted in the ribosylation of the proteins with molecular weight of 24-25 kDa. Radio labelling of these proteins was suppressed in the presence of guanosine 5′-O-(3-thiotriphosphate) (GTP-γS), a hydrolysis-resistant analog of GTP. In the isolated arteries, ADP-ribosyltransferase C3 attenuated the relaxations to bradykinin during contractions with prostaglandin F 2α in the presence of tween 80 (non ionic detergent), but not in the absence of tween 80. CONCLUSION: Low molecular weight G-proteins of the Rho family contribute to the mechanism of relaxation induced by bradykinin.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.htmlen_HK
dc.relation.ispartofActa Pharmacologica Sinicaen_HK
dc.subjectBradykininen_HK
dc.subjectEndotheliumen_HK
dc.subjectGTP-binding proteinsen_HK
dc.subjectNitric oxideen_HK
dc.titleLow molecular weight G-proteins of rho-family mediate relaxations to bradykinin in porcine coronary arteriesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1671-4083&volume=24&issue=11&spage=1070&epage=1076&date=2003&atitle=Low+molecular+weight+G-proteins+of+rho-family+mediate+relaxations+to+bradykinin+in+porcine+coronary+arteriesen_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid14627487-
dc.identifier.scopuseid_2-s2.0-0242390523en_HK
dc.identifier.hkuros95755en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0242390523&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1070en_HK
dc.identifier.epage1076en_HK
dc.identifier.isiWOS:000186505500002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShibano, T=7006946465en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats