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Article: Interactions between panax quinquefolium saponins and vitamin C are observed in vitro

TitleInteractions between panax quinquefolium saponins and vitamin C are observed in vitro
Authors
KeywordsAntioxidant
LDL oxidation
Panax quinquefolium saponins
Vitamin C
Issue Date2000
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177
Citation
Molecular And Cellular Biochemistry, 2000, v. 204 n. 1-2, p. 77-82 How to Cite?
AbstractInasmuch as the oxidation of low-density lipoprotein (Ox-LDL) may play a key role in the initiation and progression of atherosclerosis, it has become increasingly important to identify potential antioxidants. Panax quinquefolium saponins (PQS) are extracted from the stems and leaves of the North American form of ginseng, Panax quinquefolium. Our previous studies have indicated that PQS (0.25-1 mg/ml) can protect against oxidation of LDL in vitro. The purpose of the current work was to investigate the potential interaction of lower concentrations of PQS (1-100 μg/ml) with vitamin C on the reduction of LDL oxidation. LDL was isolated from the plasma of healthy human donors by sequential ultracentrifugation. Native LDL (0.05 or 0.2 mg/ml) was incubated with PQS and/or vitamin C for 30 min at 20°C. Oxidative modification was initiated with 2 μM or 5 μM CuSO 4 at 37°C for 0-24 h. Pretreatment with PQS (100 μg/ml) reduced alterations in phospholipids, lipid peroxide levels and relative electrophoretic mobility of Ox-LDL. The presence of vitamin C (1-10 μM) significantly enhanced the protective effects of PQS. Pretreatment with PQS (1-100 μg/ml) resulted in concentration-dependent inhibition of LDL oxidation and prolongation of lag time as determined from measurements of conjugated lipid hydroperoxide content in Ox-LDL samples. Interestingly, the inhibitory actions of lower amounts of PQS (1 and 10 μg/ml) on the formation of conjugated dienes were significantly increased when vitamin C (0.1 or 1 μM) was present. In conclusion, our results suggest that PQS not only have direct antioxidant property but at low concentrations, their actions can be enhanced by vitamin C.
Persistent Identifierhttp://hdl.handle.net/10722/80204
ISSN
2015 Impact Factor: 2.613
2015 SCImago Journal Rankings: 1.019
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, JPen_HK
dc.contributor.authorHuang, Men_HK
dc.contributor.authorTeoh, Hen_HK
dc.contributor.authorMan, RYKen_HK
dc.date.accessioned2010-09-06T08:03:41Z-
dc.date.available2010-09-06T08:03:41Z-
dc.date.issued2000en_HK
dc.identifier.citationMolecular And Cellular Biochemistry, 2000, v. 204 n. 1-2, p. 77-82en_HK
dc.identifier.issn0300-8177en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80204-
dc.description.abstractInasmuch as the oxidation of low-density lipoprotein (Ox-LDL) may play a key role in the initiation and progression of atherosclerosis, it has become increasingly important to identify potential antioxidants. Panax quinquefolium saponins (PQS) are extracted from the stems and leaves of the North American form of ginseng, Panax quinquefolium. Our previous studies have indicated that PQS (0.25-1 mg/ml) can protect against oxidation of LDL in vitro. The purpose of the current work was to investigate the potential interaction of lower concentrations of PQS (1-100 μg/ml) with vitamin C on the reduction of LDL oxidation. LDL was isolated from the plasma of healthy human donors by sequential ultracentrifugation. Native LDL (0.05 or 0.2 mg/ml) was incubated with PQS and/or vitamin C for 30 min at 20°C. Oxidative modification was initiated with 2 μM or 5 μM CuSO 4 at 37°C for 0-24 h. Pretreatment with PQS (100 μg/ml) reduced alterations in phospholipids, lipid peroxide levels and relative electrophoretic mobility of Ox-LDL. The presence of vitamin C (1-10 μM) significantly enhanced the protective effects of PQS. Pretreatment with PQS (1-100 μg/ml) resulted in concentration-dependent inhibition of LDL oxidation and prolongation of lag time as determined from measurements of conjugated lipid hydroperoxide content in Ox-LDL samples. Interestingly, the inhibitory actions of lower amounts of PQS (1 and 10 μg/ml) on the formation of conjugated dienes were significantly increased when vitamin C (0.1 or 1 μM) was present. In conclusion, our results suggest that PQS not only have direct antioxidant property but at low concentrations, their actions can be enhanced by vitamin C.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177en_HK
dc.relation.ispartofMolecular and Cellular Biochemistryen_HK
dc.subjectAntioxidanten_HK
dc.subjectLDL oxidationen_HK
dc.subjectPanax quinquefolium saponinsen_HK
dc.subjectVitamin Cen_HK
dc.titleInteractions between panax quinquefolium saponins and vitamin C are observed in vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-8177&volume=204&spage=77&epage=82&date=2000&atitle=Interactions+between+panax+quinquefolium+saponins+and+vitamin+C+are+observed+in+vitroen_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10718627-
dc.identifier.scopuseid_2-s2.0-0034002971en_HK
dc.identifier.hkuros50174en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034002971&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume204en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage77en_HK
dc.identifier.epage82en_HK
dc.identifier.isiWOS:000085463000010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, JP=12796026200en_HK
dc.identifier.scopusauthoridHuang, M=55198346200en_HK
dc.identifier.scopusauthoridTeoh, H=7003816542en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK

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