File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Growth inhibition and cell cycle arrest effects of epigallocatechin gallate in the NBT-II bladder tumour cell line

TitleGrowth inhibition and cell cycle arrest effects of epigallocatechin gallate in the NBT-II bladder tumour cell line
Authors
KeywordsBladder cancer
Cell cycle
Cyclin D1
Epigallocatechin gallate
Green tea
Issue Date2004
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJU
Citation
Bju International, 2004, v. 93 n. 7, p. 1082-1086 How to Cite?
AbstractOBJECTIVES: To examine the growth inhibition and cell cycle arrest effects of epigallocatechin gallate (EGCG), a major constituent of green tea polyphenols, on the NBT-II bladder tumour cell line. MATERIALS AND METHODS: Growth inhibition and cell cycle arrest effects of EGCG were evaluated by the tetrazolium assay, flow cytometry and apoptotic DNA ladder tests. The cell cycle-related oncogene and protein expressions in NBT-II bladder tumour cells, when incubated with EGCG, were detected with reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. RESULTS: EGCG inhibited growth of the NBT-II bladder tumour cells in a dose- and time-dependent manner. Flow cytometry showed a G0/G1 arrest in cells when cultured with EGCG at doses of 10, 20 or 40 μmol/L for 48 or 72 h. The apoptotic DNA ladder test showed that EGCG at 10 μmol/L induced early apoptosis after 48 h of incubation. A down-regulation of cyclin D1 was detected by RT-PCR when the cells were incubated with EGCG (20 μmol/L for 48 h. EGCG also down-regulated protein expression of cyclin D1, cyclin-dependent kinase 4/6 and phosphorylated retinoblastoma protein, in both a time- and dose-dependent manner, when detected by Western blot. CONCLUSION: EGCG had growth inhibition and cell-cycle arrest effects in NBT-II bladder tumour cells by down-regulating the cyclin D1, cyclin-dependent kinase 4/6 and retinoblastoma protein machinery for regulating cell-cycle progression.
Persistent Identifierhttp://hdl.handle.net/10722/80203
ISSN
2015 Impact Factor: 4.387
2015 SCImago Journal Rankings: 2.009
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, JJen_HK
dc.contributor.authorYe, ZQen_HK
dc.contributor.authorKoo, MWLen_HK
dc.date.accessioned2010-09-06T08:03:40Z-
dc.date.available2010-09-06T08:03:40Z-
dc.date.issued2004en_HK
dc.identifier.citationBju International, 2004, v. 93 n. 7, p. 1082-1086en_HK
dc.identifier.issn1464-4096en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80203-
dc.description.abstractOBJECTIVES: To examine the growth inhibition and cell cycle arrest effects of epigallocatechin gallate (EGCG), a major constituent of green tea polyphenols, on the NBT-II bladder tumour cell line. MATERIALS AND METHODS: Growth inhibition and cell cycle arrest effects of EGCG were evaluated by the tetrazolium assay, flow cytometry and apoptotic DNA ladder tests. The cell cycle-related oncogene and protein expressions in NBT-II bladder tumour cells, when incubated with EGCG, were detected with reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. RESULTS: EGCG inhibited growth of the NBT-II bladder tumour cells in a dose- and time-dependent manner. Flow cytometry showed a G0/G1 arrest in cells when cultured with EGCG at doses of 10, 20 or 40 μmol/L for 48 or 72 h. The apoptotic DNA ladder test showed that EGCG at 10 μmol/L induced early apoptosis after 48 h of incubation. A down-regulation of cyclin D1 was detected by RT-PCR when the cells were incubated with EGCG (20 μmol/L for 48 h. EGCG also down-regulated protein expression of cyclin D1, cyclin-dependent kinase 4/6 and phosphorylated retinoblastoma protein, in both a time- and dose-dependent manner, when detected by Western blot. CONCLUSION: EGCG had growth inhibition and cell-cycle arrest effects in NBT-II bladder tumour cells by down-regulating the cyclin D1, cyclin-dependent kinase 4/6 and retinoblastoma protein machinery for regulating cell-cycle progression.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJUen_HK
dc.relation.ispartofBJU Internationalen_HK
dc.rightsB J U International. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectBladder canceren_HK
dc.subjectCell cycleen_HK
dc.subjectCyclin D1en_HK
dc.subjectEpigallocatechin gallateen_HK
dc.subjectGreen teaen_HK
dc.titleGrowth inhibition and cell cycle arrest effects of epigallocatechin gallate in the NBT-II bladder tumour cell lineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1464-4096&volume=93&spage=1082&epage=1086&date=2004&atitle=Growth+inhibition+and+cell+cycle+arrest+effects+of+epigallocatechin+gallate+in+the+NBT-II+bladder+tumour+cell+lineen_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1464-410X.2004.04785.xen_HK
dc.identifier.pmid15142168-
dc.identifier.scopuseid_2-s2.0-2542511546en_HK
dc.identifier.hkuros87562en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2542511546&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume93en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1082en_HK
dc.identifier.epage1086en_HK
dc.identifier.isiWOS:000221254400036-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChen, JJ=7501885932en_HK
dc.identifier.scopusauthoridYe, ZQ=7401956734en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats