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Article: Cells derived from regenerated endothelium of the porcine coronary artery contain more oxidized forms of apolipoprotein-B-100 without a modification in the uptake of oxidized LDL

TitleCells derived from regenerated endothelium of the porcine coronary artery contain more oxidized forms of apolipoprotein-B-100 without a modification in the uptake of oxidized LDL
Authors
KeywordsCholesterol
Endothelial cells
Low-density lipoprotein
Oxidation
Regenerated endothelium
Issue Date2003
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR
Citation
Journal Of Vascular Research, 2003, v. 40 n. 4, p. 389-398 How to Cite?
AbstractIncreased accumulation of lipoproteins and cholesterol within cells from regenerated endothelium may be responsible for their reported dysfunction. This study compared the presence and uptake of oxidized forms of low-density lipoprotein (LDL) in cells derived from native and regenerated endothelium. Four weeks after balloon denudation, primary cultures of native and regenerated endothelial cells were prepared from porcine coronary arteries. Regenerated endothelium stained more strongly using an antibody against oxidized lipoproteins. The increase in oxidized forms of apolipoprotein-B-100 exhibited by cells from regenerated endothelium was not due to an increase in extracellular-induced oxidation of native LDL, measured as the production of thiobarbituric-acid-reactive substances, being videntical in both cell types. Intracellular cholesterol and cholesterol ester content were unchanged in regenerated cells. Using flow cytometry, accumulation of oxidized LDL was investigated further by quantifying the uptake of a mildly oxidized preparation of 1,1'-dioctadecyl-3,3,3',3-tetra-methyl-indocarbocyanine perchlorate-labelled LDL. The parameters of uptake, EC50 and Emax, were not different between cells from native and regenerated endothelium suggesting that the number of LOX-1 receptors was identical in the two cell types. Moreover, a negative correlation between the increased uptake of acetylated LDL and decreased cGMP production in response to bradykinin was observed in cells from regenerated endothelium. Thus, the increased incorporation of modified LDL and their intracellular oxidation could be responsible for the alteration in NO production. The presence of oxidized forms of LDL may be a marker of endothelium regeneration and could be involved in the endothelial dysfunction of pig coronary arteries 4 weeks after balloon denudation. Copyright © 2003 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/80196
ISSN
2015 Impact Factor: 2.186
2015 SCImago Journal Rankings: 1.119
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKennedy, Sen_HK
dc.contributor.authorFournetBourguignon, MPen_HK
dc.contributor.authorBreugnot, Cen_HK
dc.contributor.authorCastedoDelrieu, Men_HK
dc.contributor.authorLesage, Len_HK
dc.contributor.authorReure, Hen_HK
dc.contributor.authorBriant, Cen_HK
dc.contributor.authorLeonce, Sen_HK
dc.contributor.authorVilaine, JPen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-06T08:03:35Z-
dc.date.available2010-09-06T08:03:35Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Vascular Research, 2003, v. 40 n. 4, p. 389-398en_HK
dc.identifier.issn1018-1172en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80196-
dc.description.abstractIncreased accumulation of lipoproteins and cholesterol within cells from regenerated endothelium may be responsible for their reported dysfunction. This study compared the presence and uptake of oxidized forms of low-density lipoprotein (LDL) in cells derived from native and regenerated endothelium. Four weeks after balloon denudation, primary cultures of native and regenerated endothelial cells were prepared from porcine coronary arteries. Regenerated endothelium stained more strongly using an antibody against oxidized lipoproteins. The increase in oxidized forms of apolipoprotein-B-100 exhibited by cells from regenerated endothelium was not due to an increase in extracellular-induced oxidation of native LDL, measured as the production of thiobarbituric-acid-reactive substances, being videntical in both cell types. Intracellular cholesterol and cholesterol ester content were unchanged in regenerated cells. Using flow cytometry, accumulation of oxidized LDL was investigated further by quantifying the uptake of a mildly oxidized preparation of 1,1'-dioctadecyl-3,3,3',3-tetra-methyl-indocarbocyanine perchlorate-labelled LDL. The parameters of uptake, EC50 and Emax, were not different between cells from native and regenerated endothelium suggesting that the number of LOX-1 receptors was identical in the two cell types. Moreover, a negative correlation between the increased uptake of acetylated LDL and decreased cGMP production in response to bradykinin was observed in cells from regenerated endothelium. Thus, the increased incorporation of modified LDL and their intracellular oxidation could be responsible for the alteration in NO production. The presence of oxidized forms of LDL may be a marker of endothelium regeneration and could be involved in the endothelial dysfunction of pig coronary arteries 4 weeks after balloon denudation. Copyright © 2003 S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVRen_HK
dc.relation.ispartofJournal of Vascular Researchen_HK
dc.rightsJournal of Vascular Research. Copyright © S Karger AG.en_HK
dc.subjectCholesterolen_HK
dc.subjectEndothelial cellsen_HK
dc.subjectLow-density lipoproteinen_HK
dc.subjectOxidationen_HK
dc.subjectRegenerated endotheliumen_HK
dc.titleCells derived from regenerated endothelium of the porcine coronary artery contain more oxidized forms of apolipoprotein-B-100 without a modification in the uptake of oxidized LDLen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1018-1172&volume=40&spage=389&epage=398&date=2003&atitle=Cells+Derived+from+Regenerated+Endothelium+of+the+Porcine+Coronary+Artery+Contain+More+Oxidized+Forms+of+Apolipoprotein-B-100+without+a+Modification+in+the+Uptake+of+Oxidized+LDLen_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000072817en_HK
dc.identifier.pmid12904688-
dc.identifier.scopuseid_2-s2.0-0141728440en_HK
dc.identifier.hkuros95764en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141728440&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue4en_HK
dc.identifier.spage389en_HK
dc.identifier.epage398en_HK
dc.identifier.isiWOS:000185735800009-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridKennedy, S=7401494232en_HK
dc.identifier.scopusauthoridFournetBourguignon, MP=6507287770en_HK
dc.identifier.scopusauthoridBreugnot, C=6603192478en_HK
dc.identifier.scopusauthoridCastedoDelrieu, M=6507843556en_HK
dc.identifier.scopusauthoridLesage, L=7004179797en_HK
dc.identifier.scopusauthoridReure, H=6506882220en_HK
dc.identifier.scopusauthoridBriant, C=8722899600en_HK
dc.identifier.scopusauthoridLeonce, S=7005463951en_HK
dc.identifier.scopusauthoridVilaine, JP=7004617134en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK

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