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Article: Expression and immunolocalization of heat shock proteins in the healing of gastric ulcers in rats

TitleExpression and immunolocalization of heat shock proteins in the healing of gastric ulcers in rats
Authors
KeywordsGastric ulcer
Heat shock proteins
Issue Date2002
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00365521.asp
Citation
Scandinavian Journal Of Gastroenterology, 2002, v. 37 n. 1, p. 17-22 How to Cite?
AbstractBackground: Heat shock proteins are induced when cells are subjected to noxious stimuli. They afford cytoprotection and increase the resistance of the tissue to damage. However, their roles in the healing of gastric ulcers have not been well established. In this study, the expression and immunolocalization of three heat shock proteins (HSPs); namely inducible HSP70 (iHSP70), HSP47, and HSP32 during ulcer healing were investigated in rats with gastric ulcer. Methods: Gastric ulcers (kissing ulcers) were induced by luminal application of acetic acid solution. Gastric tissue samples were obtained from the ulcer base, ulcer margin, and non-ulcerated area around the ulcer margin at different time intervals after ulcer induction. The protein levels and distributions of HSPs were analyzed with Western blotting and immunohistochemical methods, respectively. Results: It was found that all HSPs were expressed in normal, non-ulcerated, and gastric ulcer tissues. HSP32 was elevated during inflammation (1-8 days after ulcer induction), while HSP47 expression was exacerbated at the ulcer base and margin during ulcer healing (3-12 days). Decreased expression of iHSP70 was observed at the ulcer base immediately after ulcer induction, but returned to normal level by the end of the healing stage (8-12 days). Inducible HSP70 was found distributed in the gastric glands and injured tissues in the inflamed areas. Wide distribution of HSP47 was detected in granulation tissues and collagen producing cells, while HSP32 was localized in the gastric glands and inflammatory cells. Conclusions: The findings indicate that iHSP70, HSP47, and HSP32 play different roles during ulcer healing. HSP32 seems to act as an inflammatory defensive factor, and HSP47 as a collagen-specific molecular chaperon contributing significantly to gastric ulcer healing. However, the role of iHSP70 in the ulcer healing process is still undefined.
Persistent Identifierhttp://hdl.handle.net/10722/80190
ISSN
2015 Impact Factor: 2.199
2015 SCImago Journal Rankings: 0.891
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuo, JSen_HK
dc.contributor.authorCho, CHen_HK
dc.contributor.authorWang, JYen_HK
dc.contributor.authorKoo, MWLen_HK
dc.date.accessioned2010-09-06T08:03:30Z-
dc.date.available2010-09-06T08:03:30Z-
dc.date.issued2002en_HK
dc.identifier.citationScandinavian Journal Of Gastroenterology, 2002, v. 37 n. 1, p. 17-22en_HK
dc.identifier.issn0036-5521en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80190-
dc.description.abstractBackground: Heat shock proteins are induced when cells are subjected to noxious stimuli. They afford cytoprotection and increase the resistance of the tissue to damage. However, their roles in the healing of gastric ulcers have not been well established. In this study, the expression and immunolocalization of three heat shock proteins (HSPs); namely inducible HSP70 (iHSP70), HSP47, and HSP32 during ulcer healing were investigated in rats with gastric ulcer. Methods: Gastric ulcers (kissing ulcers) were induced by luminal application of acetic acid solution. Gastric tissue samples were obtained from the ulcer base, ulcer margin, and non-ulcerated area around the ulcer margin at different time intervals after ulcer induction. The protein levels and distributions of HSPs were analyzed with Western blotting and immunohistochemical methods, respectively. Results: It was found that all HSPs were expressed in normal, non-ulcerated, and gastric ulcer tissues. HSP32 was elevated during inflammation (1-8 days after ulcer induction), while HSP47 expression was exacerbated at the ulcer base and margin during ulcer healing (3-12 days). Decreased expression of iHSP70 was observed at the ulcer base immediately after ulcer induction, but returned to normal level by the end of the healing stage (8-12 days). Inducible HSP70 was found distributed in the gastric glands and injured tissues in the inflamed areas. Wide distribution of HSP47 was detected in granulation tissues and collagen producing cells, while HSP32 was localized in the gastric glands and inflammatory cells. Conclusions: The findings indicate that iHSP70, HSP47, and HSP32 play different roles during ulcer healing. HSP32 seems to act as an inflammatory defensive factor, and HSP47 as a collagen-specific molecular chaperon contributing significantly to gastric ulcer healing. However, the role of iHSP70 in the ulcer healing process is still undefined.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00365521.aspen_HK
dc.relation.ispartofScandinavian Journal of Gastroenterologyen_HK
dc.rightsScandinavian Journal of Gastroenterology. Copyright © Informa Healthcare.en_HK
dc.subjectGastric ulceren_HK
dc.subjectHeat shock proteinsen_HK
dc.titleExpression and immunolocalization of heat shock proteins in the healing of gastric ulcers in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0036-5521&volume=37&spage=17&epage=22&date=2002&atitle=Expression+and+immunolocalization+of+heat+shock+proteins+in+the+healing+of+gastric+ulcers+in+ratsen_HK
dc.identifier.emailKoo, MWL: wlkoo@hku.hken_HK
dc.identifier.authorityKoo, MWL=rp00233en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/003655202753387293en_HK
dc.identifier.pmid11858169en_HK
dc.identifier.scopuseid_2-s2.0-0036146933en_HK
dc.identifier.hkuros73764en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036146933&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue1en_HK
dc.identifier.spage17en_HK
dc.identifier.epage22en_HK
dc.identifier.isiWOS:000173299900004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGuo, JS=7404488815en_HK
dc.identifier.scopusauthoridCho, CH=14067000400en_HK
dc.identifier.scopusauthoridWang, JY=21136381300en_HK
dc.identifier.scopusauthoridKoo, MWL=7004550899en_HK

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