File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Maternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34 + cells

TitleMaternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34 + cells
Authors
KeywordsCD34 + cells
Haemopoietic primitive cell
Human serum albumin
Maternal plasma
Umbilical cord blood
Issue Date2007
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH
Citation
British Journal Of Haematology, 2007, v. 137 n. 5, p. 468-474 How to Cite?
AbstractUmbilical cord blood is a valuable source of haemopoietic stem/progenitor cells (HSC) for transplantation. This study explored the effect of maternal plasma/human serum albumin (HSA) in the purification and culture conditions of CD34 + cells derived from human umbilical cord blood. During CD34 + cell enrichment, including maternal plasma or HSA instead of fetal bovine serum (FBS) in the wash buffer, significantly increased the purity and the fold expansion of CD34 + cells. The increase in fold expansion of CD34 + cells was independent of CD34 + cell purity before expansion. With FBS, the mean fold expansion of CD34 + cells and total nucleated cells on day 7 was 9.7 ± 5.5 and 39.7 ± 13.7 respectively. The use of maternal plasma increased the mean fold expansion of CD34 + cells and total nucleated cells on day 7 to 28.2 ± 6.7 and 71.5 ± 15.4 respectively. When HSA was added to wash buffer, the mean fold expansion of CD34 + cells and total nucleated cells were 30.4 ± 10.5 and 83.5 ± 24.8 respectively. No statistical significance was found between using HSA and maternal plasma on total cell and CD34 + cell expansion. We propose that HSA in maternal plasma was responsible for the positive effect on CD34 + cell enrichment and expansion. © 2007 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/80129
ISSN
2015 Impact Factor: 5.401
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKwok, YKen_HK
dc.contributor.authorTang, MHYen_HK
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorNgai, CSen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorLau, ETen_HK
dc.date.accessioned2010-09-06T08:02:44Z-
dc.date.available2010-09-06T08:02:44Z-
dc.date.issued2007en_HK
dc.identifier.citationBritish Journal Of Haematology, 2007, v. 137 n. 5, p. 468-474en_HK
dc.identifier.issn0007-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80129-
dc.description.abstractUmbilical cord blood is a valuable source of haemopoietic stem/progenitor cells (HSC) for transplantation. This study explored the effect of maternal plasma/human serum albumin (HSA) in the purification and culture conditions of CD34 + cells derived from human umbilical cord blood. During CD34 + cell enrichment, including maternal plasma or HSA instead of fetal bovine serum (FBS) in the wash buffer, significantly increased the purity and the fold expansion of CD34 + cells. The increase in fold expansion of CD34 + cells was independent of CD34 + cell purity before expansion. With FBS, the mean fold expansion of CD34 + cells and total nucleated cells on day 7 was 9.7 ± 5.5 and 39.7 ± 13.7 respectively. The use of maternal plasma increased the mean fold expansion of CD34 + cells and total nucleated cells on day 7 to 28.2 ± 6.7 and 71.5 ± 15.4 respectively. When HSA was added to wash buffer, the mean fold expansion of CD34 + cells and total nucleated cells were 30.4 ± 10.5 and 83.5 ± 24.8 respectively. No statistical significance was found between using HSA and maternal plasma on total cell and CD34 + cell expansion. We propose that HSA in maternal plasma was responsible for the positive effect on CD34 + cell enrichment and expansion. © 2007 The Authors.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJHen_HK
dc.relation.ispartofBritish Journal of Haematologyen_HK
dc.rightsBritish Journal of Haematology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectCD34 + cellsen_HK
dc.subjectHaemopoietic primitive cellen_HK
dc.subjectHuman serum albuminen_HK
dc.subjectMaternal plasmaen_HK
dc.subjectUmbilical cord blooden_HK
dc.titleMaternal plasma or human serum albumin in wash buffer enhances enrichment and ex vivo expansion of human umbilical cord blood CD34 + cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1048&volume=137 &issue=5&spage=468&epage=474&date=2007&atitle=Maternal+plasma+or+human+serum+albumin+in+wash+buffer+enhances+enrichment+and+ex+vivo+expansion+of+human+umbilical+cord+blood+CD34++cellsen_HK
dc.identifier.emailTang, MHY: mhytang@hkucc.hku.hken_HK
dc.identifier.emailLau, YL: lauylung@hku.hken_HK
dc.identifier.authorityTang, MHY=rp01701en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-2141.2007.06606.xen_HK
dc.identifier.pmid17488490-
dc.identifier.scopuseid_2-s2.0-34247857331en_HK
dc.identifier.hkuros129295en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247857331&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume137en_HK
dc.identifier.issue5en_HK
dc.identifier.spage468en_HK
dc.identifier.epage474en_HK
dc.identifier.isiWOS:000246186800012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridKwok, YK=8247106700en_HK
dc.identifier.scopusauthoridTang, MHY=35362943900en_HK
dc.identifier.scopusauthoridLaw, HKW=7101939394en_HK
dc.identifier.scopusauthoridNgai, CS=6603676357en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridLau, ET=36006491400en_HK
dc.identifier.citeulike1279612-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats