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Article: Changes of CD14 and CD1a expression in response to IL-4 and granulocyte-macrophage colony-stimulating factor are different in cord blood and adult blood monocytes

TitleChanges of CD14 and CD1a expression in response to IL-4 and granulocyte-macrophage colony-stimulating factor are different in cord blood and adult blood monocytes
Authors
Issue Date2001
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
Citation
Pediatric Research, 2001, v. 50 n. 2, p. 184-189 How to Cite?
AbstractNeonates are relatively immature in their immune response; thus, to further clarify the differences of monocyte function and differentiation between neonates and adults, we investigated their CD14+CD4+ and CD14+CD16+ monocyte subpopulations, production of IL-1β and tumor necrosis factor-α induced by lipopolysaccharide, and their CD14 and CD1a phenotypic changes in response to IL-4 and granulocyte-macrophage colony-stimulating factor. Our results showed that 1) the expression of CD14 in cord blood monocytes was significantly lower than that in adult peripheral blood monocytes; 2) both the percentages of CD14+CD4+ cells and CD14+CD16+ cells among CD14+ monocytes were also significantly lower in cord blood; 3) after stimulation by lipopolysaccharide for 72 h, production of both IL-1β and tumor necrosis factor-α was lower in cord blood than that in adult peripheral blood; and 4) in response to IL-4 or GM-CSF, the phenotype development of CD14 and CD1a in cord blood and adult peripheral blood was different. Down-regulation of CD14 expression in response to IL-4 and GM-CSF was slower in cord blood monocytes than that in adult peripheral blood monocytes. After 9 d of culture in the presence of IL-4 and GM-CSF, the percentage of CD1a+ monocytes was significantly more increased in cord blood than that in adult peripheral blood. The reduced expression of CD14 and other mature phenotype markers such as CD16 and CD4 as well as the reduced IL-1β and tumor necrosis factor-α production may contribute to the impaired immune response of neonates. Slower down-regulation of CD14 by IL-4 and GM-CSF suggests that differential properties of cord blood monocytes in response to cellular stress signals take a longer time than those of adult peripheral blood monocytes.
Persistent Identifierhttp://hdl.handle.net/10722/80125
ISSN
2015 Impact Factor: 2.761
2015 SCImago Journal Rankings: 1.290
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Een_HK
dc.contributor.authorTu, Wen_HK
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2010-09-06T08:02:42Z-
dc.date.available2010-09-06T08:02:42Z-
dc.date.issued2001en_HK
dc.identifier.citationPediatric Research, 2001, v. 50 n. 2, p. 184-189en_HK
dc.identifier.issn0031-3998en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80125-
dc.description.abstractNeonates are relatively immature in their immune response; thus, to further clarify the differences of monocyte function and differentiation between neonates and adults, we investigated their CD14+CD4+ and CD14+CD16+ monocyte subpopulations, production of IL-1β and tumor necrosis factor-α induced by lipopolysaccharide, and their CD14 and CD1a phenotypic changes in response to IL-4 and granulocyte-macrophage colony-stimulating factor. Our results showed that 1) the expression of CD14 in cord blood monocytes was significantly lower than that in adult peripheral blood monocytes; 2) both the percentages of CD14+CD4+ cells and CD14+CD16+ cells among CD14+ monocytes were also significantly lower in cord blood; 3) after stimulation by lipopolysaccharide for 72 h, production of both IL-1β and tumor necrosis factor-α was lower in cord blood than that in adult peripheral blood; and 4) in response to IL-4 or GM-CSF, the phenotype development of CD14 and CD1a in cord blood and adult peripheral blood was different. Down-regulation of CD14 expression in response to IL-4 and GM-CSF was slower in cord blood monocytes than that in adult peripheral blood monocytes. After 9 d of culture in the presence of IL-4 and GM-CSF, the percentage of CD1a+ monocytes was significantly more increased in cord blood than that in adult peripheral blood. The reduced expression of CD14 and other mature phenotype markers such as CD16 and CD4 as well as the reduced IL-1β and tumor necrosis factor-α production may contribute to the impaired immune response of neonates. Slower down-regulation of CD14 by IL-4 and GM-CSF suggests that differential properties of cord blood monocytes in response to cellular stress signals take a longer time than those of adult peripheral blood monocytes.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/en_HK
dc.relation.ispartofPediatric Researchen_HK
dc.rightsPediatric Research. Copyright © Lippincott Williams & Wilkins.en_HK
dc.titleChanges of CD14 and CD1a expression in response to IL-4 and granulocyte-macrophage colony-stimulating factor are different in cord blood and adult blood monocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-3998&volume=50&issue=2&spage=184&epage=189&date=2001&atitle=Changes+of+CD14+and+CD1a+Expression+in+Response+to+IL-4+and+Granulocyte-Macrophage+Colony-Stimulating+Factor+Are+Different+in+Cord+Blood+and+Adult+Blood+Monocytesen_HK
dc.identifier.emailTu, W:wwtu@hkucc.hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.authorityTu, W=rp00416en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11477201-
dc.identifier.scopuseid_2-s2.0-0034918931en_HK
dc.identifier.hkuros65000en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034918931&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume50en_HK
dc.identifier.issue2en_HK
dc.identifier.spage184en_HK
dc.identifier.epage189en_HK
dc.identifier.isiWOS:000170096800004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, E=7202240063en_HK
dc.identifier.scopusauthoridTu, W=7006479236en_HK
dc.identifier.scopusauthoridLaw, HKW=7101939394en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK

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