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Article: Therapeutic expression of an anti-death receptor 5 single-chain fixed-variable region prevents tumor growth in mice

TitleTherapeutic expression of an anti-death receptor 5 single-chain fixed-variable region prevents tumor growth in mice
Authors
Issue Date2006
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 2006, v. 66 n. 24, p. 11946-11953 How to Cite?
AbstractThe clinical use of the single-chain fixed-variable (scFv) fragments of recombinant monoclonal antibodies as credible alternatives for classic therapeutic antibodies has two limitations: rapid blood clearance and inefficient local expression of functional molecules. In attempt to address these issues, we have developed a novel gene therapy protocol in which the anti-death receptor 5 (DR5) scFv fragments were either in vitro expressed in several tumor cell lines, or in vivo expressed in mice, using recombinant adeno-associated virus (rAAV)-mediated gene transfer. Viral transduction using the rAAV-S3C construct, which encodes a scFv molecule (S3C scFv) specific to DR5, led to stable expression in tumor cell lines and showed apoptosis-inducing activity in vitro, which could be inhibited by recombinant DR5 but not by DR4. A single i.m. injection of rAAV-S3C virus in nude mice resulted in stable expression of DR5-binding S3C scFv proteins in mouse sera for at least 240 days. Moreover, the expression of S3C scFv was associated with significant suppression of tumor growth and the increase of tumor cell apoptosis in previously established s.c. human lung LTEP-sml and liver Hep3B tumor xenografts. ©2006 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/80110
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShi, Jen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorZheng, Yen_HK
dc.contributor.authorGuo, Yen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorCheung, PTen_HK
dc.contributor.authorXu, Ren_HK
dc.contributor.authorZheng, Den_HK
dc.date.accessioned2010-09-06T08:02:32Z-
dc.date.available2010-09-06T08:02:32Z-
dc.date.issued2006en_HK
dc.identifier.citationCancer Research, 2006, v. 66 n. 24, p. 11946-11953en_HK
dc.identifier.issn0008-5472en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80110-
dc.description.abstractThe clinical use of the single-chain fixed-variable (scFv) fragments of recombinant monoclonal antibodies as credible alternatives for classic therapeutic antibodies has two limitations: rapid blood clearance and inefficient local expression of functional molecules. In attempt to address these issues, we have developed a novel gene therapy protocol in which the anti-death receptor 5 (DR5) scFv fragments were either in vitro expressed in several tumor cell lines, or in vivo expressed in mice, using recombinant adeno-associated virus (rAAV)-mediated gene transfer. Viral transduction using the rAAV-S3C construct, which encodes a scFv molecule (S3C scFv) specific to DR5, led to stable expression in tumor cell lines and showed apoptosis-inducing activity in vitro, which could be inhibited by recombinant DR5 but not by DR4. A single i.m. injection of rAAV-S3C virus in nude mice resulted in stable expression of DR5-binding S3C scFv proteins in mouse sera for at least 240 days. Moreover, the expression of S3C scFv was associated with significant suppression of tumor growth and the increase of tumor cell apoptosis in previously established s.c. human lung LTEP-sml and liver Hep3B tumor xenografts. ©2006 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_HK
dc.relation.ispartofCancer Researchen_HK
dc.titleTherapeutic expression of an anti-death receptor 5 single-chain fixed-variable region prevents tumor growth in miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=66&issue=24&spage=11946&epage=11953&date=2006&atitle=Therapeutic+Expression+of+an+Anti-Death+Receptor+5+Single-Chain+Fixed-Variable+Region+Prevents+Tumor+Growth+in+Miceen_HK
dc.identifier.emailCheung, PT:ptcheung@hkucc.hku.hken_HK
dc.identifier.authorityCheung, PT=rp00351en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1158/0008-5472.CAN-06-1227en_HK
dc.identifier.pmid17178893en_HK
dc.identifier.scopuseid_2-s2.0-33846260583en_HK
dc.identifier.hkuros127715en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846260583&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue24en_HK
dc.identifier.spage11946en_HK
dc.identifier.epage11953en_HK
dc.identifier.isiWOS:000242915600053-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShi, J=7404495444en_HK
dc.identifier.scopusauthoridLiu, Y=8232975800en_HK
dc.identifier.scopusauthoridZheng, Y=7404837714en_HK
dc.identifier.scopusauthoridGuo, Y=10540367500en_HK
dc.identifier.scopusauthoridZhang, J=15766639800en_HK
dc.identifier.scopusauthoridCheung, PT=7202595465en_HK
dc.identifier.scopusauthoridXu, R=16426308500en_HK
dc.identifier.scopusauthoridZheng, D=7202567084en_HK

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