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Article: Isolation of differentially expressed genes in human heart tissues

TitleIsolation of differentially expressed genes in human heart tissues
Authors
KeywordsCongenital heart defect
Dystrophin
Gene expression
RNA arbitrarily primed-PCR
Titin
Issue Date2002
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbadis
Citation
Biochimica Et Biophysica Acta - Molecular Basis Of Disease, 2002, v. 1588 n. 3, p. 241-246 How to Cite?
AbstractWe applied RNA arbitrarily primed-PCR (RAP-PCR) to screen the genes differentially expressed between common congenital heart defects (CHD) [atrial septal defect, ventricular septal defect, Tetrology of Fallot (TOF)] and normal human heart samples. Three of these differentially amplified fragments matched cDNA sequences coding for proteins of unknown function in humans: hCALO (human homologue of calossin), NP79 (coding for a nuclear protein of 79KD) and SUN2 (Sad-1 unc-84 domain protein 2). The other four fragments were from known human genes: apolipoprotein J, titin, dystrophin and protein kinase C-delta. Northern blot analysis confirmed that all of these genes are expressed in the human heart. The results of RAP-PCR were reconfirmed by quantitative RT-PCR in TOF and control heart samples. Both techniques showed the levels of expression of hCALO, NP79 and SUN2 to be comparable in TOF and control samples and the level of expression of dystrophin and titin, both coding for cytoskeletal proteins, to be significantly upregulated in TOF samples. In summary, we have shown that the RAP-PCR technique is useful in the identification of differentially expressed gene from biopsy samples of human CHD tissues. In this manner, we have identified three novel genes implicated in the normal function of the human heart and two known genes upregulated in TOF samples. © 2002 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/80057
ISSN
2015 Impact Factor: 5.158
2015 SCImago Journal Rankings: 2.718
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, Gen_HK
dc.contributor.authorYuen Chan, Sen_HK
dc.contributor.authorYuan, Yen_HK
dc.contributor.authorWang Chan, Ken_HK
dc.contributor.authorQiu, Gen_HK
dc.contributor.authorSun, Ken_HK
dc.contributor.authorPing Leung, Men_HK
dc.date.accessioned2010-09-06T08:01:52Z-
dc.date.available2010-09-06T08:01:52Z-
dc.date.issued2002en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Basis Of Disease, 2002, v. 1588 n. 3, p. 241-246en_HK
dc.identifier.issn0925-4439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80057-
dc.description.abstractWe applied RNA arbitrarily primed-PCR (RAP-PCR) to screen the genes differentially expressed between common congenital heart defects (CHD) [atrial septal defect, ventricular septal defect, Tetrology of Fallot (TOF)] and normal human heart samples. Three of these differentially amplified fragments matched cDNA sequences coding for proteins of unknown function in humans: hCALO (human homologue of calossin), NP79 (coding for a nuclear protein of 79KD) and SUN2 (Sad-1 unc-84 domain protein 2). The other four fragments were from known human genes: apolipoprotein J, titin, dystrophin and protein kinase C-delta. Northern blot analysis confirmed that all of these genes are expressed in the human heart. The results of RAP-PCR were reconfirmed by quantitative RT-PCR in TOF and control heart samples. Both techniques showed the levels of expression of hCALO, NP79 and SUN2 to be comparable in TOF and control samples and the level of expression of dystrophin and titin, both coding for cytoskeletal proteins, to be significantly upregulated in TOF samples. In summary, we have shown that the RAP-PCR technique is useful in the identification of differentially expressed gene from biopsy samples of human CHD tissues. In this manner, we have identified three novel genes implicated in the normal function of the human heart and two known genes upregulated in TOF samples. © 2002 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbadisen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Basis of Diseaseen_HK
dc.rightsB B A - Molecular Basis of Disease. Copyright © Elsevier BV.en_HK
dc.subjectCongenital heart defecten_HK
dc.subjectDystrophinen_HK
dc.subjectGene expressionen_HK
dc.subjectRNA arbitrarily primed-PCRen_HK
dc.subjectTitinen_HK
dc.titleIsolation of differentially expressed genes in human heart tissuesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0925-4439&volume=1588&spage=241&epage=246&date=2002&atitle=Isolation+of+differentially+expressed+genes+in+human+heart+tissuesen_HK
dc.identifier.emailYuen Chan, S:sychan@hkucc.hku.hken_HK
dc.identifier.authorityYuen Chan, S=rp00356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0925-4439(02)00171-0en_HK
dc.identifier.pmid12393179-
dc.identifier.scopuseid_2-s2.0-0037069735en_HK
dc.identifier.hkuros75006en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037069735&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1588en_HK
dc.identifier.issue3en_HK
dc.identifier.spage241en_HK
dc.identifier.epage246en_HK
dc.identifier.isiWOS:000179025500007-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridSun, G=7402760357en_HK
dc.identifier.scopusauthoridYuen Chan, S=7404255082en_HK
dc.identifier.scopusauthoridYuan, Y=12794596600en_HK
dc.identifier.scopusauthoridWang Chan, K=15828663500en_HK
dc.identifier.scopusauthoridQiu, G=7103292117en_HK
dc.identifier.scopusauthoridSun, K=14012221500en_HK
dc.identifier.scopusauthoridPing Leung, M=15828122300en_HK

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