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Article: Semiquantitative immunoblots of membrane protein-epidermal growth factor

TitleSemiquantitative immunoblots of membrane protein-epidermal growth factor
Authors
KeywordsEGF
Immunoblot
Protein extraction method
Issue Date2000
PublisherHumana Press, Inc.
Citation
Applied Biochemistry And Biotechnology - Part B Molecular Biotechnology, 2000, v. 15 n. 1, p. 65-67 How to Cite?
AbstractMembrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract as a stimulator of eyelid opening and incisor eruption when injected into newborn mice and rats. The EGF precursor is a transmembrane protein with eight additional EGF-like repeats. Our previous study has shown that the EGF precursor without these eight EGF-like repeats (hEGF) was biologically active. Here, we introduce a modified method for rapid detection of hEGF. The membranous protein was directly extracted from various organs of transgenic mice (including the submandibular gland, kidney, liver, heart, and testis) with two different buffers and easily detected by semiquantitative immunoblotting.
Persistent Identifierhttp://hdl.handle.net/10722/80053
ISSN
2015 Impact Factor: 1.752
2015 SCImago Journal Rankings: 0.787
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, RWCen_HK
dc.contributor.authorChan, SYen_HK
dc.date.accessioned2010-09-06T08:01:49Z-
dc.date.available2010-09-06T08:01:49Z-
dc.date.issued2000en_HK
dc.identifier.citationApplied Biochemistry And Biotechnology - Part B Molecular Biotechnology, 2000, v. 15 n. 1, p. 65-67en_HK
dc.identifier.issn1073-6085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80053-
dc.description.abstractMembrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract as a stimulator of eyelid opening and incisor eruption when injected into newborn mice and rats. The EGF precursor is a transmembrane protein with eight additional EGF-like repeats. Our previous study has shown that the EGF precursor without these eight EGF-like repeats (hEGF) was biologically active. Here, we introduce a modified method for rapid detection of hEGF. The membranous protein was directly extracted from various organs of transgenic mice (including the submandibular gland, kidney, liver, heart, and testis) with two different buffers and easily detected by semiquantitative immunoblotting.en_HK
dc.languageengen_HK
dc.publisherHumana Press, Inc.en_HK
dc.relation.ispartofApplied Biochemistry and Biotechnology - Part B Molecular Biotechnologyen_HK
dc.subjectEGFen_HK
dc.subjectImmunobloten_HK
dc.subjectProtein extraction methoden_HK
dc.titleSemiquantitative immunoblots of membrane protein-epidermal growth factoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1073-6085&volume=15&spage=65&epage=67&date=2000&atitle=Semiquantitative+Immunoblots+of+Membrane+Protein-Epidermal+Growth+Factoren_HK
dc.identifier.emailChan, SY:sychan@hkucc.hku.hken_HK
dc.identifier.authorityChan, SY=rp00356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10911623-
dc.identifier.scopuseid_2-s2.0-0033945619en_HK
dc.identifier.hkuros54318en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033945619&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue1en_HK
dc.identifier.spage65en_HK
dc.identifier.epage67en_HK
dc.identifier.isiWOS:000087873600007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, RWC=13007985800en_HK
dc.identifier.scopusauthoridChan, SY=7404255082en_HK

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