File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Decreased yield, phenotypic expression and function of immature monocyte-derived dendritic cells in cord blood

TitleDecreased yield, phenotypic expression and function of immature monocyte-derived dendritic cells in cord blood
Authors
KeywordsCord blood
Dendritic cell
Endocytosis
Mixed lymphocyte reaction
Monocyte
Issue Date2001
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH
Citation
British Journal Of Haematology, 2001, v. 113 n. 1, p. 240-246 How to Cite?
AbstractDendritic cells are critical for the induction of both primary immune responses and immunological tolerance, as well as for the regulation of T-helper 1 (Th1) and 2 (Th2) immune responses. As neonates are notably deficient in Th1 response and cord blood transplantation is noted to result in less graft-versus-host disease (GvHD), we compared the phenotypic and functional characteristics of monocyte-derived dendritic cells (DCs) that favour Th1 development from cord blood and adult peripheral blood to understand the underlying mechanisms of these observations. Our results showed that: (1) after culture for 7 d with interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF), cord blood monocytes generated less CD1a+ cells than adult peripheral blood monocytes, and the CD1a+ cell percentage decreased thereafter: (2) compared with adult blood DCs, cord blood DCs had reduced intensity of expression of CD1a and MHC class II molecules, but the expression levels of CD11c and CD86 were similar; (3) the endocytotic ability of cord blood DCs was reduced compared with adult blood DCs, and this function was related to reduced mannose receptor (MR)-positive cells; (4) furthermore, the ability of cord blood DCs to stimulate CD3+ T cells in an allogeneic mixed lymphocyte reaction was significantly lower than that of adult blood DCs. These results suggested that the dysfunction of cord blood monocytes in differentiating into professional DCs will affect the activation of naive T cells, especially Th1 development, and may be related to the susceptibility to different infections in the neonates, as well as the lower incidence of GvHD in cord blood transplantation.
Persistent Identifierhttp://hdl.handle.net/10722/80018
ISSN
2015 Impact Factor: 5.401
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Een_HK
dc.contributor.authorTu, Wen_HK
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2010-09-06T08:01:25Z-
dc.date.available2010-09-06T08:01:25Z-
dc.date.issued2001en_HK
dc.identifier.citationBritish Journal Of Haematology, 2001, v. 113 n. 1, p. 240-246en_HK
dc.identifier.issn0007-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/80018-
dc.description.abstractDendritic cells are critical for the induction of both primary immune responses and immunological tolerance, as well as for the regulation of T-helper 1 (Th1) and 2 (Th2) immune responses. As neonates are notably deficient in Th1 response and cord blood transplantation is noted to result in less graft-versus-host disease (GvHD), we compared the phenotypic and functional characteristics of monocyte-derived dendritic cells (DCs) that favour Th1 development from cord blood and adult peripheral blood to understand the underlying mechanisms of these observations. Our results showed that: (1) after culture for 7 d with interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF), cord blood monocytes generated less CD1a+ cells than adult peripheral blood monocytes, and the CD1a+ cell percentage decreased thereafter: (2) compared with adult blood DCs, cord blood DCs had reduced intensity of expression of CD1a and MHC class II molecules, but the expression levels of CD11c and CD86 were similar; (3) the endocytotic ability of cord blood DCs was reduced compared with adult blood DCs, and this function was related to reduced mannose receptor (MR)-positive cells; (4) furthermore, the ability of cord blood DCs to stimulate CD3+ T cells in an allogeneic mixed lymphocyte reaction was significantly lower than that of adult blood DCs. These results suggested that the dysfunction of cord blood monocytes in differentiating into professional DCs will affect the activation of naive T cells, especially Th1 development, and may be related to the susceptibility to different infections in the neonates, as well as the lower incidence of GvHD in cord blood transplantation.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJHen_HK
dc.relation.ispartofBritish Journal of Haematologyen_HK
dc.rightsBritish Journal of Haematology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectCord blooden_HK
dc.subjectDendritic cellen_HK
dc.subjectEndocytosisen_HK
dc.subjectMixed lymphocyte reactionen_HK
dc.subjectMonocyteen_HK
dc.titleDecreased yield, phenotypic expression and function of immature monocyte-derived dendritic cells in cord blooden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1048&volume=113&spage=240&epage=246&date=2001&atitle=Decreased+yield,+phenotypic+expression+and+function+of+immature+monocyte-derived+dendritic+cells+in+cord+blooden_HK
dc.identifier.emailTu, W:wwtu@hkucc.hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.authorityTu, W=rp00416en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-2141.2001.02720.xen_HK
dc.identifier.pmid11328307-
dc.identifier.scopuseid_2-s2.0-0035022218en_HK
dc.identifier.hkuros57030en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035022218&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume113en_HK
dc.identifier.issue1en_HK
dc.identifier.spage240en_HK
dc.identifier.epage246en_HK
dc.identifier.isiWOS:000168700500038-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, E=7202240063en_HK
dc.identifier.scopusauthoridTu, W=7006479236en_HK
dc.identifier.scopusauthoridLaw, HKW=7101939394en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats