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Article: Expression of epidermal growth factor in transgenic mice causes growth retardation

TitleExpression of epidermal growth factor in transgenic mice causes growth retardation
Authors
Issue Date2000
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2000, v. 275 n. 49, p. 38693-38698 How to Cite?
AbstractThe epidermal growth factor (EGF) family of peptides signals through the erbB family of receptor tyrosine kinases and plays important roles in development and tumorigenesis. Both EGF and transforming growth factor (TGF)-α only bind to erbB1 and activate it. The precursor of EGF is distinct from that of TGF-α in having eight additional EGF-like repeats. We have recently shown that the EGF precursor without these repeats is biologically active and leads to hypospermatogenesis in transgenic mice. Here we present evidence that the growth of transgenic mice widely expressing this engineered EGF precursor is also stunted. These mice were consistently born at half the normal weight and reached almost 80% of normal weight at adulthood. The mechanism involved a reduction of serum insulin-like growth factor-binding protein-3. Chondrocyte development in the growth plate was affected, and osteoblasts accumulated in the endosteum and periosteum. Besides these novel findings on the in vivo effects of EGF on bone development, we observed no sign of tumor formation in our transgenic animals. In contrast to previous reports on TGF-α transgenic mice, we show that the biological functions of EGF and TGF-α are clearly distinct.
Persistent Identifierhttp://hdl.handle.net/10722/79985
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, SYen_HK
dc.contributor.authorWong, RWCen_HK
dc.date.accessioned2010-09-06T08:01:03Z-
dc.date.available2010-09-06T08:01:03Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2000, v. 275 n. 49, p. 38693-38698en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79985-
dc.description.abstractThe epidermal growth factor (EGF) family of peptides signals through the erbB family of receptor tyrosine kinases and plays important roles in development and tumorigenesis. Both EGF and transforming growth factor (TGF)-α only bind to erbB1 and activate it. The precursor of EGF is distinct from that of TGF-α in having eight additional EGF-like repeats. We have recently shown that the EGF precursor without these repeats is biologically active and leads to hypospermatogenesis in transgenic mice. Here we present evidence that the growth of transgenic mice widely expressing this engineered EGF precursor is also stunted. These mice were consistently born at half the normal weight and reached almost 80% of normal weight at adulthood. The mechanism involved a reduction of serum insulin-like growth factor-binding protein-3. Chondrocyte development in the growth plate was affected, and osteoblasts accumulated in the endosteum and periosteum. Besides these novel findings on the in vivo effects of EGF on bone development, we observed no sign of tumor formation in our transgenic animals. In contrast to previous reports on TGF-α transgenic mice, we show that the biological functions of EGF and TGF-α are clearly distinct.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.titleExpression of epidermal growth factor in transgenic mice causes growth retardationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=275&issue=49&spage=38693&epage=38698&date=2000&atitle=Expression+of+Epidermal+Growth+Factor+in+Transgenic+Mice+Causes+Growth+Retardationen_HK
dc.identifier.emailChan, SY:sychan@hkucc.hku.hken_HK
dc.identifier.authorityChan, SY=rp00356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1074/jbc.M004189200en_HK
dc.identifier.pmid11001946-
dc.identifier.scopuseid_2-s2.0-0034623957en_HK
dc.identifier.hkuros56068en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034623957&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume275en_HK
dc.identifier.issue49en_HK
dc.identifier.spage38693en_HK
dc.identifier.epage38698en_HK
dc.identifier.isiWOS:000165739800076-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, SY=7404255082en_HK
dc.identifier.scopusauthoridWong, RWC=15745711500en_HK

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