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Article: Validation and development of quantitative flow cytometry-based fluorescence in situ hybridization for intercenter comparison of telomere length measurement

TitleValidation and development of quantitative flow cytometry-based fluorescence in situ hybridization for intercenter comparison of telomere length measurement
Authors
KeywordsCell analysis
Flow cytometry
Fluorescence in situ hybridization
Nucleic acid hybridization
Peptide nucleic acid
Q-Flow FISH
Telomere
Telomere length
Issue Date2001
PublisherWiley-Liss.
Citation
Cytometry, 2001, v. 43 n. 2, p. 150-153 How to Cite?
AbstractBackground: Telomeres are highly conserved repeats at the ends of chromosomes that maintain chromosome stability and reflect the replicative potential of cells. Telomere length can be determined by Southern blot hybridization or quantitative fluorescence in situ hybridization (Q-FISH). Recently, two flow cytometry-based (Flow) FISH protocols have been published. Methods: We compared the telomere length measured by Southern blotting and Flow FISH using standard beads to calibrate and quantify the fluorescence intensity. Results: The telomeric fluorescence of cord blood and peripheral blood mononuclear cells was similar to that reported by other studies. There was a linear relationship between the telomeric fluorescence determined by Flow FISH and the telomere fragment size determined by Southern blotting (r = 0.89; P < 0.001). Conclusion: It is important to set up a center-specific curve and select appropriate cell lines for reference. This Q-Flow FISH protocol will facilitate the measurement of telomere length and allow more meaningful comparison of data (in standard fluorescence units or fragment size) between institutes. © 2001 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/79917
ISSN
2004 Impact Factor: 2.698
2007 SCImago Journal Rankings: 0.190
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2010-09-06T08:00:16Z-
dc.date.available2010-09-06T08:00:16Z-
dc.date.issued2001en_HK
dc.identifier.citationCytometry, 2001, v. 43 n. 2, p. 150-153en_HK
dc.identifier.issn0196-4763en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79917-
dc.description.abstractBackground: Telomeres are highly conserved repeats at the ends of chromosomes that maintain chromosome stability and reflect the replicative potential of cells. Telomere length can be determined by Southern blot hybridization or quantitative fluorescence in situ hybridization (Q-FISH). Recently, two flow cytometry-based (Flow) FISH protocols have been published. Methods: We compared the telomere length measured by Southern blotting and Flow FISH using standard beads to calibrate and quantify the fluorescence intensity. Results: The telomeric fluorescence of cord blood and peripheral blood mononuclear cells was similar to that reported by other studies. There was a linear relationship between the telomeric fluorescence determined by Flow FISH and the telomere fragment size determined by Southern blotting (r = 0.89; P < 0.001). Conclusion: It is important to set up a center-specific curve and select appropriate cell lines for reference. This Q-Flow FISH protocol will facilitate the measurement of telomere length and allow more meaningful comparison of data (in standard fluorescence units or fragment size) between institutes. © 2001 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherWiley-Liss.en_HK
dc.relation.ispartofCytometryen_HK
dc.subjectCell analysisen_HK
dc.subjectFlow cytometryen_HK
dc.subjectFluorescence in situ hybridizationen_HK
dc.subjectNucleic acid hybridizationen_HK
dc.subjectPeptide nucleic aciden_HK
dc.subjectQ-Flow FISHen_HK
dc.subjectTelomereen_HK
dc.subjectTelomere lengthen_HK
dc.titleValidation and development of quantitative flow cytometry-based fluorescence in situ hybridization for intercenter comparison of telomere length measurementen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0196-4763&volume=43&issue=2&spage=150&epage=153&date=2001&atitle=Validation+and+Development+of+Quantitative+Flow+Cytometry-Based+Fluorescence+in+Situ+Hybridization+for+Intercenter+Comparison+of+Telomere+Length+Measurementen_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1097-0320(20010201)43:2<150::AID-CYTO1030>3.0.CO;2-Hen_HK
dc.identifier.pmid11169580-
dc.identifier.scopuseid_2-s2.0-0035245998en_HK
dc.identifier.hkuros56428en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035245998&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue2en_HK
dc.identifier.spage150en_HK
dc.identifier.epage153en_HK
dc.identifier.isiWOS:000166700600009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLaw, HKW=7101939394en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK

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