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Article: Humoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine

TitleHumoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine
Authors
KeywordsAntibody
Children
Influenza vaccine
T-cells
Issue Date2007
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pidj.com
Citation
Pediatric Infectious Disease Journal, 2007, v. 26 n. 2, p. 107-115 How to Cite?
AbstractBACKGROUND: There have been no prior reports of the frequency of circulating influenza-specific, interferon gamma-producing memory CD4 and CD8 T-cells in healthy children who have received multiple influenza immunizations. METHODS: We evaluated 21 previously immunized children, ages 3 to 9 years, before and 1 month after administration of trivalent inactivated influenza vaccine. Frequencies of influenza-specific CD4 and CD8 T-cells stimulated with trivalent inactivated influenza vaccine or A/Panama (H3N2) virus were determined by flow cytometry, and antibody responses to vaccine strains and a drifted H3N2 strain were measured by hemagglutination inhibition assay and neutralizing antibody assays. RESULTS: Mean change in CD4 and in CD8 T-cell frequencies after immunization was 0.01% (P > 0.39) with postimmunization CD4 frequencies higher than CD8 frequencies. Children with more previous vaccinations had a higher baseline frequency of CD4 T-cells (P = 0.0002) but a smaller increase or even a decline from baseline after immunization (P = 0.003). An association between age and change in frequency was not detected. Baseline geometric mean titers (GMTs) and seroprotection rates were significantly higher in older children against A/Panama (neutralizing baseline GMT, P = 0.0488) and A/New Caledonia (hemagglutination inhibition baseline GMT and seroprotection, P < 0.0297). Baseline GMTs against B/Hong Kong were not associated with age or quantity of prior vaccinations. CONCLUSIONS: These findings suggest that children may plateau in CD4 T-cell responses to influenza antigens with repeated exposures and that the number of exposures may play a large role in building a memory CD4 T-cell response to influenza A, perhaps independently from age. © 2007 Lippincott Williams & Wilkins, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/79889
ISSN
2015 Impact Factor: 2.587
2015 SCImago Journal Rankings: 1.416
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZeman, AMen_HK
dc.contributor.authorHolmes, THen_HK
dc.contributor.authorStamatis, Sen_HK
dc.contributor.authorTu, Wen_HK
dc.contributor.authorHe, XSen_HK
dc.contributor.authorBouvier, Nen_HK
dc.contributor.authorKemble, Gen_HK
dc.contributor.authorGreenberg, HBen_HK
dc.contributor.authorLewis, DBen_HK
dc.contributor.authorArvin, AMen_HK
dc.contributor.authorDekker, CLen_HK
dc.date.accessioned2010-09-06T07:59:53Z-
dc.date.available2010-09-06T07:59:53Z-
dc.date.issued2007en_HK
dc.identifier.citationPediatric Infectious Disease Journal, 2007, v. 26 n. 2, p. 107-115en_HK
dc.identifier.issn0891-3668en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79889-
dc.description.abstractBACKGROUND: There have been no prior reports of the frequency of circulating influenza-specific, interferon gamma-producing memory CD4 and CD8 T-cells in healthy children who have received multiple influenza immunizations. METHODS: We evaluated 21 previously immunized children, ages 3 to 9 years, before and 1 month after administration of trivalent inactivated influenza vaccine. Frequencies of influenza-specific CD4 and CD8 T-cells stimulated with trivalent inactivated influenza vaccine or A/Panama (H3N2) virus were determined by flow cytometry, and antibody responses to vaccine strains and a drifted H3N2 strain were measured by hemagglutination inhibition assay and neutralizing antibody assays. RESULTS: Mean change in CD4 and in CD8 T-cell frequencies after immunization was 0.01% (P > 0.39) with postimmunization CD4 frequencies higher than CD8 frequencies. Children with more previous vaccinations had a higher baseline frequency of CD4 T-cells (P = 0.0002) but a smaller increase or even a decline from baseline after immunization (P = 0.003). An association between age and change in frequency was not detected. Baseline geometric mean titers (GMTs) and seroprotection rates were significantly higher in older children against A/Panama (neutralizing baseline GMT, P = 0.0488) and A/New Caledonia (hemagglutination inhibition baseline GMT and seroprotection, P < 0.0297). Baseline GMTs against B/Hong Kong were not associated with age or quantity of prior vaccinations. CONCLUSIONS: These findings suggest that children may plateau in CD4 T-cell responses to influenza antigens with repeated exposures and that the number of exposures may play a large role in building a memory CD4 T-cell response to influenza A, perhaps independently from age. © 2007 Lippincott Williams & Wilkins, Inc.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pidj.comen_HK
dc.relation.ispartofPediatric Infectious Disease Journalen_HK
dc.rightsThe Pediatric Infectious Disease Journal. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subjectAntibodyen_HK
dc.subjectChildrenen_HK
dc.subjectInfluenza vaccineen_HK
dc.subjectT-cellsen_HK
dc.titleHumoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0891-3668&volume=26&spage=107&epage=15&date=2007&atitle=Humoral+And+Cellular+Immune+Responses+In+Children+Given+Annual+Immunization+With+Trivalent+Inactivated+Influenza+Vaccine.en_HK
dc.identifier.emailTu, W:wwtu@hkucc.hku.hken_HK
dc.identifier.authorityTu, W=rp00416en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/01.inf.0000253251.03785.9ben_HK
dc.identifier.pmid17259871-
dc.identifier.scopuseid_2-s2.0-33846560620en_HK
dc.identifier.hkuros129184en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846560620&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue2en_HK
dc.identifier.spage107en_HK
dc.identifier.epage115en_HK
dc.identifier.isiWOS:000243985700002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZeman, AM=35110096600en_HK
dc.identifier.scopusauthoridHolmes, TH=35857851000en_HK
dc.identifier.scopusauthoridStamatis, S=6507666397en_HK
dc.identifier.scopusauthoridTu, W=7006479236en_HK
dc.identifier.scopusauthoridHe, XS=36061249700en_HK
dc.identifier.scopusauthoridBouvier, N=15833841900en_HK
dc.identifier.scopusauthoridKemble, G=35474594000en_HK
dc.identifier.scopusauthoridGreenberg, HB=7201404942en_HK
dc.identifier.scopusauthoridLewis, DB=7404750928en_HK
dc.identifier.scopusauthoridArvin, AM=26425414800en_HK
dc.identifier.scopusauthoridDekker, CL=7005790077en_HK

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