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Article: An efficient method of generating transgenic mice by pronuclear microinjection

TitleAn efficient method of generating transgenic mice by pronuclear microinjection
Authors
KeywordsMethod
Pronuclear microinjection
Transgenic mice
Issue Date2000
PublisherHumana Press, Inc.
Citation
Applied Biochemistry And Biotechnology - Part B Molecular Biotechnology, 2000, v. 15 n. 2, p. 155-159 How to Cite?
AbstractGenetic transformation of mice using pronuclear microinjection was demonstrated by a number of groups in rapid succession in the early 1980's. Since that time, studies using transgenic animals have produced major advances in biomedical sciences and molecular genetics. More important, it is possible to study the molecular basis for tissue and stage-specific expression of genes. We recently used this method to generate transgenic mice. DNA fragment (transgene) was injected into the pronucleus of one-cell embryos. We describe this simplified protocol, which is reliable. With the use of buffered medium M2 for the whole process, it is not mandatory to have a CO2 incubator.
Persistent Identifierhttp://hdl.handle.net/10722/79819
ISSN
2015 Impact Factor: 1.752
2015 SCImago Journal Rankings: 0.787
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, RWCen_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorChan, SYen_HK
dc.date.accessioned2010-09-06T07:59:06Z-
dc.date.available2010-09-06T07:59:06Z-
dc.date.issued2000en_HK
dc.identifier.citationApplied Biochemistry And Biotechnology - Part B Molecular Biotechnology, 2000, v. 15 n. 2, p. 155-159en_HK
dc.identifier.issn1073-6085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79819-
dc.description.abstractGenetic transformation of mice using pronuclear microinjection was demonstrated by a number of groups in rapid succession in the early 1980's. Since that time, studies using transgenic animals have produced major advances in biomedical sciences and molecular genetics. More important, it is possible to study the molecular basis for tissue and stage-specific expression of genes. We recently used this method to generate transgenic mice. DNA fragment (transgene) was injected into the pronucleus of one-cell embryos. We describe this simplified protocol, which is reliable. With the use of buffered medium M2 for the whole process, it is not mandatory to have a CO2 incubator.en_HK
dc.languageengen_HK
dc.publisherHumana Press, Inc.en_HK
dc.relation.ispartofApplied Biochemistry and Biotechnology - Part B Molecular Biotechnologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectMethoden_HK
dc.subjectPronuclear microinjectionen_HK
dc.subjectTransgenic miceen_HK
dc.subject.meshAnimals-
dc.subject.meshCell Nucleus - genetics-
dc.subject.meshEpidermal Growth Factor - genetics-
dc.subject.meshMice, Transgenic - genetics-
dc.subject.meshMicroinjections - methods-
dc.titleAn efficient method of generating transgenic mice by pronuclear microinjectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1073-6085&volume=15&issue=2&spage=155&epage=159&date=2000&atitle=An+efficient+method+of+generating+transgenic+mice+by+pronuclear+microinjectionen_HK
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.emailChan, SY:sychan@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.authorityChan, SY=rp00356en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1385/MB:15:2:155-
dc.identifier.pmid10949829-
dc.identifier.scopuseid_2-s2.0-0033867590en_HK
dc.identifier.hkuros52492en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033867590&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue2en_HK
dc.identifier.spage155en_HK
dc.identifier.epage159en_HK
dc.identifier.isiWOS:000088423800008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, RWC=34875928200en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridChan, SY=7404255082en_HK

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