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Article: Insulin-Like Growth Factor I Promotes Maturation and Inhibits Apoptosis of Immature Cord Blood Monocyte-Derived Dendritic Cells through MEK and PI 3-Kinase Pathways

TitleInsulin-Like Growth Factor I Promotes Maturation and Inhibits Apoptosis of Immature Cord Blood Monocyte-Derived Dendritic Cells through MEK and PI 3-Kinase Pathways
Authors
Issue Date2003
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
Citation
Pediatric Research, 2003, v. 54 n. 6, p. 919-925 How to Cite?
AbstractIGF-I has profound effects on the immune system. We previously reported that IGF-I promoted cord blood (CB)-naïve T-cell maturation and now show that IGF-I promoted maturation of CB monocyte-derived dendritic cells (DC) with up-regulation of CD83, CD86, CD40, and major histocompatibility complex (MHC) class II molecules, and down-regulation of mannose receptor. Furthermore, IGF-I inhibited apoptosis of CB DC and increased the production of tumor necrosis factor α (TNF-α). These effects were blocked by specific mitogen-activated protein kinase kinase (MEK) inhibitor (PD98059) and phosphoinositol 3-kinase inhibitor (LY294002). PD98059 partially inhibited the IGF-I-induced up-regulation of MHC class II. In contrast, LY294002 was additive in the IGF-I-induced up-regulation of MHC class II. Moreover, LY294002 significantly increased the percentage of late apoptotic cells in CB. These results imply the involvement of different pathways for the differential regulation of co-stimulatory molecule expression and apoptosis. The addition of anti-TNF-α did not neutralize the effects of IGF-I on CB DC maturation and apoptosis. On the contrary, neutralizing TNF-α significantly increased the IGF-I-induced up-regulation of CD83 and CD40. We conclude that IGF-I has maturation and survival effects on CB DC. These effects are mediated through both MEK and PI 3-kinase pathways but not through the IGF-I induction of TNF-α production by the DC.
Persistent Identifierhttp://hdl.handle.net/10722/79773
ISSN
2015 Impact Factor: 2.761
2015 SCImago Journal Rankings: 1.290
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Een_HK
dc.contributor.authorLaw, HKWen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2010-09-06T07:58:33Z-
dc.date.available2010-09-06T07:58:33Z-
dc.date.issued2003en_HK
dc.identifier.citationPediatric Research, 2003, v. 54 n. 6, p. 919-925en_HK
dc.identifier.issn0031-3998en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79773-
dc.description.abstractIGF-I has profound effects on the immune system. We previously reported that IGF-I promoted cord blood (CB)-naïve T-cell maturation and now show that IGF-I promoted maturation of CB monocyte-derived dendritic cells (DC) with up-regulation of CD83, CD86, CD40, and major histocompatibility complex (MHC) class II molecules, and down-regulation of mannose receptor. Furthermore, IGF-I inhibited apoptosis of CB DC and increased the production of tumor necrosis factor α (TNF-α). These effects were blocked by specific mitogen-activated protein kinase kinase (MEK) inhibitor (PD98059) and phosphoinositol 3-kinase inhibitor (LY294002). PD98059 partially inhibited the IGF-I-induced up-regulation of MHC class II. In contrast, LY294002 was additive in the IGF-I-induced up-regulation of MHC class II. Moreover, LY294002 significantly increased the percentage of late apoptotic cells in CB. These results imply the involvement of different pathways for the differential regulation of co-stimulatory molecule expression and apoptosis. The addition of anti-TNF-α did not neutralize the effects of IGF-I on CB DC maturation and apoptosis. On the contrary, neutralizing TNF-α significantly increased the IGF-I-induced up-regulation of CD83 and CD40. We conclude that IGF-I has maturation and survival effects on CB DC. These effects are mediated through both MEK and PI 3-kinase pathways but not through the IGF-I induction of TNF-α production by the DC.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/en_HK
dc.relation.ispartofPediatric Researchen_HK
dc.rightsPediatric Research. Copyright © Lippincott Williams & Wilkins.en_HK
dc.titleInsulin-Like Growth Factor I Promotes Maturation and Inhibits Apoptosis of Immature Cord Blood Monocyte-Derived Dendritic Cells through MEK and PI 3-Kinase Pathwaysen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-3998&volume=54&issue=6&spage=919&epage=925&date=2003&atitle=Insulin-Like+Growth+Factor+I+Promotes+Maturation+and+Inhibits+Apoptosis+of+Immature+Cord+Blood+Monocyte-Derived+Dendritic+Cells+through+MEK+and+PI+3-Kinase+Pathwaysen_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1203/01.PDR.0000088067.04673.1Ben_HK
dc.identifier.pmid12930919-
dc.identifier.scopuseid_2-s2.0-0344845395en_HK
dc.identifier.hkuros85203en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344845395&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue6en_HK
dc.identifier.spage919en_HK
dc.identifier.epage925en_HK
dc.identifier.isiWOS:000186816100022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, E=7202240063en_HK
dc.identifier.scopusauthoridLaw, HKW=7101939394en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK

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