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Article: Adeno-associated virus-mediated bone morphogenetic protein-4 gene therapy for in vivo bone formation

TitleAdeno-associated virus-mediated bone morphogenetic protein-4 gene therapy for in vivo bone formation
Authors
KeywordsAdeno-associated virus
Bone
Bone morphogenetic protein
Gene therapy
Muscle
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2003, v. 308 n. 3, p. 636-645 How to Cite?
AbstractAdeno-associated virus (AAV) is so far the most valuable vehicle for gene therapy because it has no association with immune response and human disease. The present study was conducted to investigate the feasibility of AAV-mediated BMP4 gene transfer for bone formation. In vitro study suggested that AAV-BMP4 vectors could transduce myoblast C2C12 cells and produce osteogenic BMP4. In vivo study demonstrated that new bone formation could be induced by direct injection of AAV-BMP4 into the skeletal muscle of immunocompetent rats. Histological analysis revealed that the newly formed bone was induced through endochondral mechanism. Immunohistochemical staining further demonstrated that AAV-BMP4 gene delivery could mediate long-term transduction, and the involvement of BMP4 expression was responsible for the endochondral ossification. This study is, to our knowledge, the first report in the field of AAV-based BMP gene transfer and should be promising for clinical orthopaedic applications. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79731
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorLeong, JCYen_HK
dc.date.accessioned2010-09-06T07:57:57Z-
dc.date.available2010-09-06T07:57:57Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2003, v. 308 n. 3, p. 636-645en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/79731-
dc.description.abstractAdeno-associated virus (AAV) is so far the most valuable vehicle for gene therapy because it has no association with immune response and human disease. The present study was conducted to investigate the feasibility of AAV-mediated BMP4 gene transfer for bone formation. In vitro study suggested that AAV-BMP4 vectors could transduce myoblast C2C12 cells and produce osteogenic BMP4. In vivo study demonstrated that new bone formation could be induced by direct injection of AAV-BMP4 into the skeletal muscle of immunocompetent rats. Histological analysis revealed that the newly formed bone was induced through endochondral mechanism. Immunohistochemical staining further demonstrated that AAV-BMP4 gene delivery could mediate long-term transduction, and the involvement of BMP4 expression was responsible for the endochondral ossification. This study is, to our knowledge, the first report in the field of AAV-based BMP gene transfer and should be promising for clinical orthopaedic applications. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectAdeno-associated virusen_HK
dc.subjectBoneen_HK
dc.subjectBone morphogenetic proteinen_HK
dc.subjectGene therapyen_HK
dc.subjectMuscleen_HK
dc.titleAdeno-associated virus-mediated bone morphogenetic protein-4 gene therapy for in vivo bone formationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=308&spage=636&epage=645&date=2003&atitle=Adeno-associated+virus-mediated+bone+morphogenetic+protein-4+gene+therapy+for+in+vivo+bone+formationen_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-291X(03)01429-3en_HK
dc.identifier.pmid12914798-
dc.identifier.scopuseid_2-s2.0-0042665943en_HK
dc.identifier.hkuros87055en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042665943&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume308en_HK
dc.identifier.issue3en_HK
dc.identifier.spage636en_HK
dc.identifier.epage645en_HK
dc.identifier.isiWOS:000184945400036-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridChen, Y=8926070600en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK
dc.identifier.scopusauthoridLeong, JCY=35560782200en_HK

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