File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: In vivo new bone formation by direct transfer of adenoviral-mediated bone morphogenetic protein-4 gene

TitleIn vivo new bone formation by direct transfer of adenoviral-mediated bone morphogenetic protein-4 gene
Authors
KeywordsAdenovirus
Bonemorphogenetic protein-4
Gene therapy
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2002, v. 298 n. 1, p. 121-127 How to Cite?
AbstractPrevious studies have demonstrated that bone morphogenetic protein-4 (BMP4) could participate in vivo endochondral ossification and is one of the main local contributing factors in the early stage of fracture healing. To investigate the effectiveness of BMP4 gene transfer, we constructed an adenoviral vector, Ad-BMP4, and evaluated its osteoinduction activity both in vitro and in vivo. In vitro study suggested that this vector could efficiently transduce mouse myoblast C2C12 cells and produce osteogenic BMP4 protein, as confirmed by immunofluorescence analysis and alkaline phosphatase activity assay. For in vivo study, Ad-BMP4 was directly injected into the hind limb muscles of male athymic nude rats. Visible newbone formation under X-ray films could be detected as early as threeweeks post-injection. The bone tissue was further analyzed by histological staining and revealed a typical remodeled bone structure. In conclusion, this study is the first to establish the feasibility of adenovirus-based BMP4 gene therapy for bone regeneration. © 2002 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79325
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Yen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLeong, JCYen_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorLuk, KDKen_HK
dc.date.accessioned2010-09-06T07:53:24Z-
dc.date.available2010-09-06T07:53:24Z-
dc.date.issued2002en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2002, v. 298 n. 1, p. 121-127en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/79325-
dc.description.abstractPrevious studies have demonstrated that bone morphogenetic protein-4 (BMP4) could participate in vivo endochondral ossification and is one of the main local contributing factors in the early stage of fracture healing. To investigate the effectiveness of BMP4 gene transfer, we constructed an adenoviral vector, Ad-BMP4, and evaluated its osteoinduction activity both in vitro and in vivo. In vitro study suggested that this vector could efficiently transduce mouse myoblast C2C12 cells and produce osteogenic BMP4 protein, as confirmed by immunofluorescence analysis and alkaline phosphatase activity assay. For in vivo study, Ad-BMP4 was directly injected into the hind limb muscles of male athymic nude rats. Visible newbone formation under X-ray films could be detected as early as threeweeks post-injection. The bone tissue was further analyzed by histological staining and revealed a typical remodeled bone structure. In conclusion, this study is the first to establish the feasibility of adenovirus-based BMP4 gene therapy for bone regeneration. © 2002 Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectAdenovirusen_HK
dc.subjectBonemorphogenetic protein-4en_HK
dc.subjectGene therapyen_HK
dc.titleIn vivo new bone formation by direct transfer of adenoviral-mediated bone morphogenetic protein-4 geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=298&spage=121&epage=127&date=2002&atitle=In+vivo+new+bone+formation+by+direct+transfer+of+adenoviral-mediated+bone+morphogenetic+protein-4+geneen_HK
dc.identifier.emailChen, Y:ychenc@hkucc.hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.authorityChen, Y=rp01318en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-291X(02)02394-Xen_HK
dc.identifier.pmid12379229-
dc.identifier.scopuseid_2-s2.0-0036401270en_HK
dc.identifier.hkuros79072en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036401270&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume298en_HK
dc.identifier.issue1en_HK
dc.identifier.spage121en_HK
dc.identifier.epage127en_HK
dc.identifier.isiWOS:000178792500020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, Y=36463185300en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLeong, JCY=35560782200en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats