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Article: Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells

TitleProteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells
Authors
Issue Date2008
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2008, v. 8 n. 9, p. 1851-1858 How to Cite?
Abstract
We present the first proteomic analysis on the cellular responses to avian influenza virus (H9N2) infection in a human cell line in different time courses in order to search for target proteins for viral pathogenesis/adaptation studies. By using 2-DE coupled with MALDI-TOF MS and nanoESI-MS/MS, we identified a set of differentially expressed cellular proteins, including cytoplasmic actin, cytokeratin, prohibitin, enoyl-CoA hydratase, peptide-prolyl cis-trans isomerase A (PPIase A), chloride intracellular channel protein 1, pyruvate dehydrogenase E1 component subunit beta, adenine phosphoribosyltransferase, guanine nucleotide-binding protein subunit beta, nucleoside diphosphate kinase A, elongation factor 1-beta and splicing factor, arginine/ serine rich 1. The most significant changes in different time courses were found in cytoplasmic actin and cytokeratin, both of which constituted the major components of cytoskeleton network in the cells. The obtained data suggested a possible role of the cytoskeleton during avian influenza virus infection of mammalian cells, which might help for better understanding of the dynamics of avian influenza virus and host interaction in mammalian cell setting. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/79310
ISSN
2013 Impact Factor: 3.973
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Baptist University
DC FieldValueLanguage
dc.contributor.authorLiu, Nen_HK
dc.contributor.authorSong, Wen_HK
dc.contributor.authorWang, Pen_HK
dc.contributor.authorLee, Ken_HK
dc.contributor.authorChan, Wen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorCai, Zen_HK
dc.date.accessioned2010-09-06T07:53:09Z-
dc.date.available2010-09-06T07:53:09Z-
dc.date.issued2008en_HK
dc.identifier.citationProteomics, 2008, v. 8 n. 9, p. 1851-1858en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79310-
dc.description.abstractWe present the first proteomic analysis on the cellular responses to avian influenza virus (H9N2) infection in a human cell line in different time courses in order to search for target proteins for viral pathogenesis/adaptation studies. By using 2-DE coupled with MALDI-TOF MS and nanoESI-MS/MS, we identified a set of differentially expressed cellular proteins, including cytoplasmic actin, cytokeratin, prohibitin, enoyl-CoA hydratase, peptide-prolyl cis-trans isomerase A (PPIase A), chloride intracellular channel protein 1, pyruvate dehydrogenase E1 component subunit beta, adenine phosphoribosyltransferase, guanine nucleotide-binding protein subunit beta, nucleoside diphosphate kinase A, elongation factor 1-beta and splicing factor, arginine/ serine rich 1. The most significant changes in different time courses were found in cytoplasmic actin and cytokeratin, both of which constituted the major components of cytoskeleton network in the cells. The obtained data suggested a possible role of the cytoskeleton during avian influenza virus infection of mammalian cells, which might help for better understanding of the dynamics of avian influenza virus and host interaction in mammalian cell setting. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBirdsen_HK
dc.subject.meshCytoplasm - metabolismen_HK
dc.subject.meshCytoskeleton - metabolismen_HK
dc.subject.meshGene Expression Profiling - methodsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfluenza A Virus, H9N2 Subtype - metabolismen_HK
dc.subject.meshInfluenza in Birds - virologyen_HK
dc.subject.meshInfluenza, Human - virologyen_HK
dc.subject.meshKeratins - metabolismen_HK
dc.subject.meshMass Spectrometry - methodsen_HK
dc.subject.meshPeptides - chemistryen_HK
dc.subject.meshProteins - chemistryen_HK
dc.subject.meshProteomics - methodsen_HK
dc.subject.meshSpectrometry, Mass, Electrospray Ionizationen_HK
dc.titleProteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9853&volume=8&spage=1851&epage=1858&date=2008&atitle=Proteomics+analysis+of+differential+expression+of+cellular+proteins+in+response+to+avian+H9N2+virus+infection+in+human+cells.en_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200700757en_HK
dc.identifier.pmid18398875en_HK
dc.identifier.scopuseid_2-s2.0-43549083241en_HK
dc.identifier.hkuros147170en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-43549083241&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1851en_HK
dc.identifier.epage1858en_HK
dc.identifier.isiWOS:000255720600017-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLiu, N=36079934800en_HK
dc.identifier.scopusauthoridSong, W=23490429800en_HK
dc.identifier.scopusauthoridWang, P=13907209900en_HK
dc.identifier.scopusauthoridLee, K=14060567400en_HK
dc.identifier.scopusauthoridChan, W=7403917961en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridCai, Z=7402904946en_HK

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