File Download
  • No File Attached
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells
  • Basic View
  • Metadata View
  • XML View
TitleProteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells
 
AuthorsLiu, N2
Song, W1
Wang, P1
Lee, K2
Chan, W2
Chen, H1
Cai, Z2
 
Issue Date2008
 
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
 
CitationProteomics, 2008, v. 8 n. 9, p. 1851-1858 [How to Cite?]
DOI: http://dx.doi.org/10.1002/pmic.200700757
 
AbstractWe present the first proteomic analysis on the cellular responses to avian influenza virus (H9N2) infection in a human cell line in different time courses in order to search for target proteins for viral pathogenesis/adaptation studies. By using 2-DE coupled with MALDI-TOF MS and nanoESI-MS/MS, we identified a set of differentially expressed cellular proteins, including cytoplasmic actin, cytokeratin, prohibitin, enoyl-CoA hydratase, peptide-prolyl cis-trans isomerase A (PPIase A), chloride intracellular channel protein 1, pyruvate dehydrogenase E1 component subunit beta, adenine phosphoribosyltransferase, guanine nucleotide-binding protein subunit beta, nucleoside diphosphate kinase A, elongation factor 1-beta and splicing factor, arginine/ serine rich 1. The most significant changes in different time courses were found in cytoplasmic actin and cytokeratin, both of which constituted the major components of cytoskeleton network in the cells. The obtained data suggested a possible role of the cytoskeleton during avian influenza virus infection of mammalian cells, which might help for better understanding of the dynamics of avian influenza virus and host interaction in mammalian cell setting. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
 
ISSN1615-9853
2012 Impact Factor: 4.132
2012 SCImago Journal Rankings: 1.220
 
DOIhttp://dx.doi.org/10.1002/pmic.200700757
 
ISI Accession Number IDWOS:000255720600017
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLiu, N
 
dc.contributor.authorSong, W
 
dc.contributor.authorWang, P
 
dc.contributor.authorLee, K
 
dc.contributor.authorChan, W
 
dc.contributor.authorChen, H
 
dc.contributor.authorCai, Z
 
dc.date.accessioned2010-09-06T07:53:09Z
 
dc.date.available2010-09-06T07:53:09Z
 
dc.date.issued2008
 
dc.description.abstractWe present the first proteomic analysis on the cellular responses to avian influenza virus (H9N2) infection in a human cell line in different time courses in order to search for target proteins for viral pathogenesis/adaptation studies. By using 2-DE coupled with MALDI-TOF MS and nanoESI-MS/MS, we identified a set of differentially expressed cellular proteins, including cytoplasmic actin, cytokeratin, prohibitin, enoyl-CoA hydratase, peptide-prolyl cis-trans isomerase A (PPIase A), chloride intracellular channel protein 1, pyruvate dehydrogenase E1 component subunit beta, adenine phosphoribosyltransferase, guanine nucleotide-binding protein subunit beta, nucleoside diphosphate kinase A, elongation factor 1-beta and splicing factor, arginine/ serine rich 1. The most significant changes in different time courses were found in cytoplasmic actin and cytokeratin, both of which constituted the major components of cytoskeleton network in the cells. The obtained data suggested a possible role of the cytoskeleton during avian influenza virus infection of mammalian cells, which might help for better understanding of the dynamics of avian influenza virus and host interaction in mammalian cell setting. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationProteomics, 2008, v. 8 n. 9, p. 1851-1858 [How to Cite?]
DOI: http://dx.doi.org/10.1002/pmic.200700757
 
dc.identifier.doihttp://dx.doi.org/10.1002/pmic.200700757
 
dc.identifier.epage1858
 
dc.identifier.hkuros147170
 
dc.identifier.isiWOS:000255720600017
 
dc.identifier.issn1615-9853
2012 Impact Factor: 4.132
2012 SCImago Journal Rankings: 1.220
 
dc.identifier.issue9
 
dc.identifier.openurl
 
dc.identifier.pmid18398875
 
dc.identifier.scopuseid_2-s2.0-43549083241
 
dc.identifier.spage1851
 
dc.identifier.urihttp://hdl.handle.net/10722/79310
 
dc.identifier.volume8
 
dc.languageeng
 
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
 
dc.publisher.placeGermany
 
dc.relation.ispartofProteomics
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshBirds
 
dc.subject.meshCytoplasm - metabolism
 
dc.subject.meshCytoskeleton - metabolism
 
dc.subject.meshGene Expression Profiling - methods
 
dc.subject.meshHumans
 
dc.subject.meshInfluenza A Virus, H9N2 Subtype - metabolism
 
dc.subject.meshInfluenza in Birds - virology
 
dc.subject.meshInfluenza, Human - virology
 
dc.subject.meshKeratins - metabolism
 
dc.subject.meshMass Spectrometry - methods
 
dc.subject.meshPeptides - chemistry
 
dc.subject.meshProteins - chemistry
 
dc.subject.meshProteomics - methods
 
dc.subject.meshSpectrometry, Mass, Electrospray Ionization
 
dc.titleProteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Liu, N</contributor.author>
<contributor.author>Song, W</contributor.author>
<contributor.author>Wang, P</contributor.author>
<contributor.author>Lee, K</contributor.author>
<contributor.author>Chan, W</contributor.author>
<contributor.author>Chen, H</contributor.author>
<contributor.author>Cai, Z</contributor.author>
<date.accessioned>2010-09-06T07:53:09Z</date.accessioned>
<date.available>2010-09-06T07:53:09Z</date.available>
<date.issued>2008</date.issued>
<identifier.citation>Proteomics, 2008, v. 8 n. 9, p. 1851-1858</identifier.citation>
<identifier.issn>1615-9853</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/79310</identifier.uri>
<description.abstract>We present the first proteomic analysis on the cellular responses to avian influenza virus (H9N2) infection in a human cell line in different time courses in order to search for target proteins for viral pathogenesis/adaptation studies. By using 2-DE coupled with MALDI-TOF MS and nanoESI-MS/MS, we identified a set of differentially expressed cellular proteins, including cytoplasmic actin, cytokeratin, prohibitin, enoyl-CoA hydratase, peptide-prolyl cis-trans isomerase A (PPIase A), chloride intracellular channel protein 1, pyruvate dehydrogenase E1 component subunit beta, adenine phosphoribosyltransferase, guanine nucleotide-binding protein subunit beta, nucleoside diphosphate kinase A, elongation factor 1-beta and splicing factor, arginine/ serine rich 1. The most significant changes in different time courses were found in cytoplasmic actin and cytokeratin, both of which constituted the major components of cytoskeleton network in the cells. The obtained data suggested a possible role of the cytoskeleton during avian influenza virus infection of mammalian cells, which might help for better understanding of the dynamics of avian influenza virus and host interaction in mammalian cell setting. &#169; 2008 Wiley-VCH Verlag GmbH &amp; Co. KGaA.</description.abstract>
<language>eng</language>
<publisher>Wiley - V C H Verlag GmbH &amp; Co KGaA. The Journal&apos;s web site is located at http://www.wiley-vch.de/home/proteomics</publisher>
<relation.ispartof>Proteomics</relation.ispartof>
<subject.mesh>Animals</subject.mesh>
<subject.mesh>Birds</subject.mesh>
<subject.mesh>Cytoplasm - metabolism</subject.mesh>
<subject.mesh>Cytoskeleton - metabolism</subject.mesh>
<subject.mesh>Gene Expression Profiling - methods</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Influenza A Virus, H9N2 Subtype - metabolism</subject.mesh>
<subject.mesh>Influenza in Birds - virology</subject.mesh>
<subject.mesh>Influenza, Human - virology</subject.mesh>
<subject.mesh>Keratins - metabolism</subject.mesh>
<subject.mesh>Mass Spectrometry - methods</subject.mesh>
<subject.mesh>Peptides - chemistry</subject.mesh>
<subject.mesh>Proteins - chemistry</subject.mesh>
<subject.mesh>Proteomics - methods</subject.mesh>
<subject.mesh>Spectrometry, Mass, Electrospray Ionization</subject.mesh>
<title>Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=1615-9853&amp;volume=8&amp;spage=1851&amp;epage=1858&amp;date=2008&amp;atitle=Proteomics+analysis+of+differential+expression+of+cellular+proteins+in+response+to+avian+H9N2+virus+infection+in+human+cells.</identifier.openurl>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1002/pmic.200700757</identifier.doi>
<identifier.pmid>18398875</identifier.pmid>
<identifier.scopus>eid_2-s2.0-43549083241</identifier.scopus>
<identifier.hkuros>147170</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-43549083241&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>8</identifier.volume>
<identifier.issue>9</identifier.issue>
<identifier.spage>1851</identifier.spage>
<identifier.epage>1858</identifier.epage>
<identifier.isi>WOS:000255720600017</identifier.isi>
<publisher.place>Germany</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Baptist University