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Article: Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes

TitleReliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes
Authors
KeywordsConsensus primers
Influenza virus
Polymerase chain reaction
Polymerase genes
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal Of Virological Methods, 2007, v. 142 n. 1-2, p. 218-222 How to Cite?
AbstractRealizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79301
ISSN
2015 Impact Factor: 1.508
2015 SCImago Journal Rankings: 0.866
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, OTWen_HK
dc.contributor.authorBarr, Ien_HK
dc.contributor.authorLeung, CYHen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorPoon, LLMen_HK
dc.date.accessioned2010-09-06T07:53:02Z-
dc.date.available2010-09-06T07:53:02Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Virological Methods, 2007, v. 142 n. 1-2, p. 218-222en_HK
dc.identifier.issn0166-0934en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79301-
dc.description.abstractRealizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_HK
dc.relation.ispartofJournal of Virological Methodsen_HK
dc.rightsJournal of Virological Methods. Copyright © Elsevier BV.en_HK
dc.subjectConsensus primersen_HK
dc.subjectInfluenza virusen_HK
dc.subjectPolymerase chain reactionen_HK
dc.subjectPolymerase genesen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshConsensus Sequenceen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - classification - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfluenza A virus - classification - enzymology - geneticsen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshRNA Replicase - chemistry - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methodsen_HK
dc.subject.meshViral Proteins - chemistry - geneticsen_HK
dc.titleReliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-0934&volume=142&issue=1-2&spage=218&epage=22&date=2007&atitle=Reliable+universal+RT-PCR+assays+for+studying+influenza+polymerase+subunit+gene+sequences+from+all+16+haemagglutinin+subtypesen_HK
dc.identifier.emailLeung, CYH: cyhleung@hkucc.hku.hken_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.authorityLeung, CYH=rp00307en_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jviromet.2007.01.015en_HK
dc.identifier.pmid17324474-
dc.identifier.scopuseid_2-s2.0-34247163576en_HK
dc.identifier.hkuros134612en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247163576&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume142en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage218en_HK
dc.identifier.epage222en_HK
dc.identifier.isiWOS:000246675900029-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, OTW=16230718400en_HK
dc.identifier.scopusauthoridBarr, I=7004651416en_HK
dc.identifier.scopusauthoridLeung, CYH=26531438300en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK

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