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Article: Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates

TitleRecurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates
Authors
KeywordsAdaptation
Mutation
SARS coronavirus
Selection
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 2005, v. 76 n. 4, p. 435-440 How to Cite?
AbstractFour clinical isolates of SARS coronavirus were serially passaged in two primate cell lines (FRhK4 and Vero E6). Viral genetic sequences encoding for structural proteins and open reading frames 6-8 were determined in the original clinical specimen, the initial virus isolate (passage 0) and at passages 5, 10, and 15. After 15 passages, a total of 15 different mutations were identified and 12 of them were non-synonymous mutations. Seven of these mutations were recurrent mutation and all located at the spike, membrane, and Orf 8a protein encoding sequences. Mutations in the membrane protein and a deletion in ORF 6-8 were already observed in passage 0, suggesting these amino acid substitutions are important in the adaptation of the virus isolate in primate cell culture. A mutation in the spike gene (residue 24079) appeared to be unique to adaptation in FRhK4 cells. It is important to be aware of cell culture associated mutations when interpreting data on molecular evolution of SARS coronavirus. © 2005 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/79300
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorLeung, CSWen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2010-09-06T07:53:01Z-
dc.date.available2010-09-06T07:53:01Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Medical Virology, 2005, v. 76 n. 4, p. 435-440en_HK
dc.identifier.issn0146-6615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79300-
dc.description.abstractFour clinical isolates of SARS coronavirus were serially passaged in two primate cell lines (FRhK4 and Vero E6). Viral genetic sequences encoding for structural proteins and open reading frames 6-8 were determined in the original clinical specimen, the initial virus isolate (passage 0) and at passages 5, 10, and 15. After 15 passages, a total of 15 different mutations were identified and 12 of them were non-synonymous mutations. Seven of these mutations were recurrent mutation and all located at the spike, membrane, and Orf 8a protein encoding sequences. Mutations in the membrane protein and a deletion in ORF 6-8 were already observed in passage 0, suggesting these amino acid substitutions are important in the adaptation of the virus isolate in primate cell culture. A mutation in the spike gene (residue 24079) appeared to be unique to adaptation in FRhK4 cells. It is important to be aware of cell culture associated mutations when interpreting data on molecular evolution of SARS coronavirus. © 2005 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_HK
dc.relation.ispartofJournal of Medical Virologyen_HK
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectAdaptationen_HK
dc.subjectMutationen_HK
dc.subjectSARS coronavirusen_HK
dc.subjectSelectionen_HK
dc.subject.meshAdaptation, Biologicalen_HK
dc.subject.meshAmino Acid Substitutionen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDNA, Viral - chemistry - geneticsen_HK
dc.subject.meshHaplorhinien_HK
dc.subject.meshMembrane Glycoproteins - geneticsen_HK
dc.subject.meshMutationen_HK
dc.subject.meshNucleocapsid Proteins - geneticsen_HK
dc.subject.meshOpen Reading Framesen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshSARS Virus - genetics - growth & development - isolation & purificationen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSerial Passageen_HK
dc.subject.meshSevere Acute Respiratory Syndrome - virologyen_HK
dc.subject.meshViral Envelope Proteins - geneticsen_HK
dc.subject.meshViral Matrix Proteins - geneticsen_HK
dc.titleRecurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primatesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0146-6615&volume=76&spage=435&epage=40&date=2005&atitle=Recurrent+mutations+associated+with+isolation+and+passage+of+SARS+coronavirus+in+cells+from+non-human+primates.en_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jmv.20379en_HK
dc.identifier.pmid15977248en_HK
dc.identifier.scopuseid_2-s2.0-21744446897en_HK
dc.identifier.hkuros109249en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-21744446897&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume76en_HK
dc.identifier.issue4en_HK
dc.identifier.spage435en_HK
dc.identifier.epage440en_HK
dc.identifier.isiWOS:000230215000001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridLeung, CSW=7402612654en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK

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