File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Preparation and identification of monoclonal antibodies against Penicillium marneffei

TitlePreparation and identification of monoclonal antibodies against Penicillium marneffei
Authors
Issue Date2003
Publisher第一軍醫大學. The Journal's web site is located at http://dyjd.chinajournal.net.cn/
Citation
Di Yi Jun Yi Da Xue Xue Bao = Academic Journal Of The First Medical College Of Pla, 2003, v. 23 n. 1, p. 38-39 How to Cite?
AbstractOBJECTIVE: To prepare and identify monoclonal antibodies (mAbs) against Penicillium marneffei. METHODS: Recombinant mannoprotein1 (MP1) of Penicillium marneffei was used to immunize BALB/c mice, and anti-MP1 mAbs were obtained by means of hybridoma. Screening and identification of the mAbs were subsequently performed with indirect enzyme-linked immunosorbent assay and Western blotting, respectively. RESULTS: Four hybridomas producing antibodies against Penicillium marneffei were obtained, and the IgG isotypes of the 4 mAbs were identified as IgG1 with affinity constants (K) of 8.2x10(-9), 4.7x10(-9), 6.5x10(-9) and 2.7x10(-9), respectively. Western blotting demonstrated specific recognition of Aspergillus fumigatus MP1 by the obtained mAbs. CONCLUSION: The 4 hybridomas producing anti-MP1 mAbs with high specificity and affinity can be of significant value in the diagnosis of Penicilliosis marneffei infections.
Persistent Identifierhttp://hdl.handle.net/10722/79294
ISSN

 

DC FieldValueLanguage
dc.contributor.authorQiu, LWen_HK
dc.contributor.authorPeng, DXen_HK
dc.contributor.authorXu, Hen_HK
dc.contributor.authorJia, Jen_HK
dc.contributor.authorChe, XYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:52:57Z-
dc.date.available2010-09-06T07:52:57Z-
dc.date.issued2003en_HK
dc.identifier.citationDi Yi Jun Yi Da Xue Xue Bao = Academic Journal Of The First Medical College Of Pla, 2003, v. 23 n. 1, p. 38-39en_HK
dc.identifier.issn1000-2588en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79294-
dc.description.abstractOBJECTIVE: To prepare and identify monoclonal antibodies (mAbs) against Penicillium marneffei. METHODS: Recombinant mannoprotein1 (MP1) of Penicillium marneffei was used to immunize BALB/c mice, and anti-MP1 mAbs were obtained by means of hybridoma. Screening and identification of the mAbs were subsequently performed with indirect enzyme-linked immunosorbent assay and Western blotting, respectively. RESULTS: Four hybridomas producing antibodies against Penicillium marneffei were obtained, and the IgG isotypes of the 4 mAbs were identified as IgG1 with affinity constants (K) of 8.2x10(-9), 4.7x10(-9), 6.5x10(-9) and 2.7x10(-9), respectively. Western blotting demonstrated specific recognition of Aspergillus fumigatus MP1 by the obtained mAbs. CONCLUSION: The 4 hybridomas producing anti-MP1 mAbs with high specificity and affinity can be of significant value in the diagnosis of Penicilliosis marneffei infections.en_HK
dc.languageengen_HK
dc.publisher第一軍醫大學. The Journal's web site is located at http://dyjd.chinajournal.net.cn/zh_HK
dc.relation.ispartofDi yi jun yi da xue xue bao = Academic journal of the First Medical College of PLAen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntibodies, Fungal - biosynthesis - immunologyen_HK
dc.subject.meshAntibodies, Monoclonal - biosynthesis - immunologyen_HK
dc.subject.meshAntibody Specificityen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHybridomas - immunologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshPenicillium - immunologyen_HK
dc.titlePreparation and identification of monoclonal antibodies against Penicillium marneffeien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1000-2588&volume=23&spage=38&epage=9&date=2003&atitle=Preparation+and+identification+of+monoclonal+antibodies+against+Penicillium+marneffeien_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12527512-
dc.identifier.scopuseid_2-s2.0-0141790748en_HK
dc.identifier.hkuros87971en_HK
dc.identifier.volume23en_HK
dc.identifier.issue1en_HK
dc.identifier.spage38en_HK
dc.identifier.epage39en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridQiu, LW=8719102700en_HK
dc.identifier.scopusauthoridPeng, DX=7202530618en_HK
dc.identifier.scopusauthoridXu, H=8401769300en_HK
dc.identifier.scopusauthoridJia, J=36865539700en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats