Article: Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia

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TitleRelative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
AuthorsWoo, PCY1
Lau, SKP1
Tsoi, HW1
Chan, KH1
Wong, BHL1
Che, XY3
Tam, VKP1
Tam, SCF2
Cheng, VCC1
Hung, IFN1
Wong, SSY1
Zheng, BJ1
Guan, Y1
Yuen, KY1
Issue Date2004
PublisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
CitationLancet, 2004, v. 363 n. 9412, p. 841-845 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0140-6736(04)15729-2
AbstractBackground Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94·3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0·48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.
ISSN0140-6736
2011 Impact Factor: 38.278
2011 SCImago Journal Rankings: 1.486
DOIhttp://dx.doi.org/10.1016/S0140-6736(04)15729-2
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWoo, PCY
dc.contributor.authorLau, SKP
dc.contributor.authorTsoi, HW
dc.contributor.authorChan, KH
dc.contributor.authorWong, BHL
dc.contributor.authorChe, XY
dc.contributor.authorTam, VKP
dc.contributor.authorTam, SCF
dc.contributor.authorCheng, VCC
dc.contributor.authorHung, IFN
dc.contributor.authorWong, SSY
dc.contributor.authorZheng, BJ
dc.contributor.authorGuan, Y
dc.contributor.authorYuen, KY
dc.date.accessioned2010-09-06T07:52:53Z
dc.date.available2010-09-06T07:52:53Z
dc.date.issued2004
dc.description.abstractBackground Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94·3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0·48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationLancet, 2004, v. 363 n. 9412, p. 841-845 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0140-6736(04)15729-2
dc.identifier.doihttp://dx.doi.org/10.1016/S0140-6736(04)15729-2
dc.identifier.epage845
dc.identifier.hkuros87953
dc.identifier.isiWOS:000220231500008
dc.identifier.issn0140-6736
2011 Impact Factor: 38.278
2011 SCImago Journal Rankings: 1.486
dc.identifier.issue9412
dc.identifier.openurl
dc.identifier.pmid15031027
dc.identifier.scopuseid_2-s2.0-12144286541
dc.identifier.spage841
dc.identifier.urihttp://hdl.handle.net/10722/79289
dc.identifier.volume363
dc.languageeng
dc.publisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
dc.publisher.placeUnited Kingdom
dc.relation.ispartofLancet
dc.relation.referencesReferences in Scopus
dc.subject.meshBlood Donors
dc.subject.meshBlotting, Western
dc.subject.meshChina - epidemiology
dc.subject.meshCoronavirus - genetics - isolation & purification
dc.subject.meshCross Infection - epidemiology - immunology
dc.subject.meshEnzyme-Linked Immunosorbent Assay
dc.subject.meshHumans
dc.subject.meshImmunoglobulin G - analysis - immunology
dc.subject.meshMembrane Glycoproteins - analysis
dc.subject.meshNucleocapsid Proteins - genetics - immunology
dc.subject.meshPneumonia, Viral - epidemiology - immunology - virology
dc.subject.meshRecombinant Proteins - analysis - immunology
dc.subject.meshSeroepidemiologic Studies
dc.subject.meshSevere Acute Respiratory Syndrome - epidemiology - immunology - virology
dc.subject.meshViral Envelope Proteins - analysis
dc.titleRelative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Queen Mary Hospital Hong Kong
  3. Zhujiang Hospital