File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
  • Basic View
  • Metadata View
  • XML View
TitleRelative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
 
AuthorsWoo, PCY1
Lau, SKP1
Tsoi, HW1
Chan, KH1
Wong, BHL1
Che, XY3
Tam, VKP1
Tam, SCF2
Cheng, VCC1
Hung, IFN1
Wong, SSY1
Zheng, BJ1
Guan, Y1
Yuen, KY1
 
Issue Date2004
 
PublisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
 
CitationLancet, 2004, v. 363 n. 9412, p. 841-845 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0140-6736(04)15729-2
 
AbstractBackground Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94·3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0·48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.
 
ISSN0140-6736
2012 Impact Factor: 39.06
2012 SCImago Journal Rankings: 7.074
 
DOIhttp://dx.doi.org/10.1016/S0140-6736(04)15729-2
 
ISI Accession Number IDWOS:000220231500008
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWoo, PCY
 
dc.contributor.authorLau, SKP
 
dc.contributor.authorTsoi, HW
 
dc.contributor.authorChan, KH
 
dc.contributor.authorWong, BHL
 
dc.contributor.authorChe, XY
 
dc.contributor.authorTam, VKP
 
dc.contributor.authorTam, SCF
 
dc.contributor.authorCheng, VCC
 
dc.contributor.authorHung, IFN
 
dc.contributor.authorWong, SSY
 
dc.contributor.authorZheng, BJ
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2010-09-06T07:52:53Z
 
dc.date.available2010-09-06T07:52:53Z
 
dc.date.issued2004
 
dc.description.abstractBackground Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94·3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0·48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationLancet, 2004, v. 363 n. 9412, p. 841-845 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0140-6736(04)15729-2
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0140-6736(04)15729-2
 
dc.identifier.epage845
 
dc.identifier.hkuros87953
 
dc.identifier.isiWOS:000220231500008
 
dc.identifier.issn0140-6736
2012 Impact Factor: 39.06
2012 SCImago Journal Rankings: 7.074
 
dc.identifier.issue9412
 
dc.identifier.openurl
 
dc.identifier.pmid15031027
 
dc.identifier.scopuseid_2-s2.0-12144286541
 
dc.identifier.spage841
 
dc.identifier.urihttp://hdl.handle.net/10722/79289
 
dc.identifier.volume363
 
dc.languageeng
 
dc.publisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofLancet
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBlood Donors
 
dc.subject.meshBlotting, Western
 
dc.subject.meshChina - epidemiology
 
dc.subject.meshCoronavirus - genetics - isolation & purification
 
dc.subject.meshCross Infection - epidemiology - immunology
 
dc.subject.meshEnzyme-Linked Immunosorbent Assay
 
dc.subject.meshHumans
 
dc.subject.meshImmunoglobulin G - analysis - immunology
 
dc.subject.meshMembrane Glycoproteins - analysis
 
dc.subject.meshNucleocapsid Proteins - genetics - immunology
 
dc.subject.meshPneumonia, Viral - epidemiology - immunology - virology
 
dc.subject.meshRecombinant Proteins - analysis - immunology
 
dc.subject.meshSeroepidemiologic Studies
 
dc.subject.meshSevere Acute Respiratory Syndrome - epidemiology - immunology - virology
 
dc.subject.meshViral Envelope Proteins - analysis
 
dc.titleRelative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Woo, PCY</contributor.author>
<contributor.author>Lau, SKP</contributor.author>
<contributor.author>Tsoi, HW</contributor.author>
<contributor.author>Chan, KH</contributor.author>
<contributor.author>Wong, BHL</contributor.author>
<contributor.author>Che, XY</contributor.author>
<contributor.author>Tam, VKP</contributor.author>
<contributor.author>Tam, SCF</contributor.author>
<contributor.author>Cheng, VCC</contributor.author>
<contributor.author>Hung, IFN</contributor.author>
<contributor.author>Wong, SSY</contributor.author>
<contributor.author>Zheng, BJ</contributor.author>
<contributor.author>Guan, Y</contributor.author>
<contributor.author>Yuen, KY</contributor.author>
<date.accessioned>2010-09-06T07:52:53Z</date.accessioned>
<date.available>2010-09-06T07:52:53Z</date.available>
<date.issued>2004</date.issued>
<identifier.citation>Lancet, 2004, v. 363 n. 9412, p. 841-845</identifier.citation>
<identifier.issn>0140-6736</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/79289</identifier.uri>
<description.abstract>Background Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94&#183;3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0&#183;48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.</description.abstract>
<language>eng</language>
<publisher>The Lancet Publishing Group. The Journal&apos;s web site is located at http://www.elsevier.com/locate/lancet</publisher>
<relation.ispartof>Lancet</relation.ispartof>
<subject.mesh>Blood Donors</subject.mesh>
<subject.mesh>Blotting, Western</subject.mesh>
<subject.mesh>China - epidemiology</subject.mesh>
<subject.mesh>Coronavirus - genetics - isolation &amp; purification</subject.mesh>
<subject.mesh>Cross Infection - epidemiology - immunology</subject.mesh>
<subject.mesh>Enzyme-Linked Immunosorbent Assay</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Immunoglobulin G - analysis - immunology</subject.mesh>
<subject.mesh>Membrane Glycoproteins - analysis</subject.mesh>
<subject.mesh>Nucleocapsid Proteins - genetics - immunology</subject.mesh>
<subject.mesh>Pneumonia, Viral - epidemiology - immunology - virology</subject.mesh>
<subject.mesh>Recombinant Proteins - analysis - immunology</subject.mesh>
<subject.mesh>Seroepidemiologic Studies</subject.mesh>
<subject.mesh>Severe Acute Respiratory Syndrome - epidemiology - immunology - virology</subject.mesh>
<subject.mesh>Viral Envelope Proteins - analysis</subject.mesh>
<title>Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=0140-6736&amp;volume=363&amp;spage=841&amp;epage=5&amp;date=2004&amp;atitle=Relative+rates+of+non-pneumonic+SARS+coronavirus+infection+and+SARS+coronavirus+pneumonia</identifier.openurl>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1016/S0140-6736(04)15729-2</identifier.doi>
<identifier.pmid>15031027</identifier.pmid>
<identifier.scopus>eid_2-s2.0-12144286541</identifier.scopus>
<identifier.hkuros>87953</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-12144286541&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>363</identifier.volume>
<identifier.issue>9412</identifier.issue>
<identifier.spage>841</identifier.spage>
<identifier.epage>845</identifier.epage>
<identifier.isi>WOS:000220231500008</identifier.isi>
<publisher.place>United Kingdom</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong
  2. Queen Mary Hospital Hong Kong
  3. Zhujiang Hospital