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Article: Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia

TitleRelative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia
Authors
Issue Date2004
PublisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
Citation
Lancet, 2004, v. 363 n. 9412, p. 841-845 How to Cite?
Abstract
Background Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94·3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0·48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.
Persistent Identifierhttp://hdl.handle.net/10722/79289
ISSN
2013 Impact Factor: 39.207
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorWong, BHLen_HK
dc.contributor.authorChe, XYen_HK
dc.contributor.authorTam, VKPen_HK
dc.contributor.authorTam, SCFen_HK
dc.contributor.authorCheng, VCCen_HK
dc.contributor.authorHung, IFNen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:52:53Z-
dc.date.available2010-09-06T07:52:53Z-
dc.date.issued2004en_HK
dc.identifier.citationLancet, 2004, v. 363 n. 9412, p. 841-845en_HK
dc.identifier.issn0140-6736en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79289-
dc.description.abstractBackground Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94·3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0·48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality.en_HK
dc.languageengen_HK
dc.publisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lanceten_HK
dc.relation.ispartofLanceten_HK
dc.subject.meshBlood Donorsen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshChina - epidemiologyen_HK
dc.subject.meshCoronavirus - genetics - isolation & purificationen_HK
dc.subject.meshCross Infection - epidemiology - immunologyen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoglobulin G - analysis - immunologyen_HK
dc.subject.meshMembrane Glycoproteins - analysisen_HK
dc.subject.meshNucleocapsid Proteins - genetics - immunologyen_HK
dc.subject.meshPneumonia, Viral - epidemiology - immunology - virologyen_HK
dc.subject.meshRecombinant Proteins - analysis - immunologyen_HK
dc.subject.meshSeroepidemiologic Studiesen_HK
dc.subject.meshSevere Acute Respiratory Syndrome - epidemiology - immunology - virologyen_HK
dc.subject.meshViral Envelope Proteins - analysisen_HK
dc.titleRelative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumoniaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0140-6736&volume=363&spage=841&epage=5&date=2004&atitle=Relative+rates+of+non-pneumonic+SARS+coronavirus+infection+and+SARS+coronavirus+pneumoniaen_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW: hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailHung, IFN: ivanhung@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY: samsonsy@hkucc.hku.hken_HK
dc.identifier.emailZheng, BJ: bzheng@hkucc.hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityHung, IFN=rp00508en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0140-6736(04)15729-2en_HK
dc.identifier.pmid15031027en_HK
dc.identifier.scopuseid_2-s2.0-12144286541en_HK
dc.identifier.hkuros87953en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12144286541&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume363en_HK
dc.identifier.issue9412en_HK
dc.identifier.spage841en_HK
dc.identifier.epage845en_HK
dc.identifier.isiWOS:000220231500008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridWong, BHL=7402023413en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK
dc.identifier.scopusauthoridTam, VKP=35861800700en_HK
dc.identifier.scopusauthoridTam, SCF=7202037323en_HK
dc.identifier.scopusauthoridCheng, VCC=23670479400en_HK
dc.identifier.scopusauthoridHung, IFN=7006103457en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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