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Article: Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage

TitleUnique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage
Authors
KeywordsGenome organization
Nidovirus
Replicase
RNA processing
Subgenomic mRNA synthesis
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmb
Citation
Journal Of Molecular Biology, 2003, v. 331 n. 5, p. 991-1004 How to Cite?
AbstractThe genome organization and expression strategy of the newly identified severe acute respiratory syndrome coronavirus (SARS-CoV) were predicted using recently published genome sequences. Fourteen putative open reading frames were identified, 12 of which were predicted to be expressed from a nested set of eight subgenomic mRNAs. The synthesis of these mRNAs in SARS-CoV-infected cells was confirmed experimentally. The 4382- and 7073 amino acid residue SARS-CoV replicase polyproteins are predicted to be cleaved into 16 subunits by two viral proteinases (bringing the total number of SARS-CoV proteins to 28). A phylogenetic analysis of the replicase gene, using a distantly related torovirus as an outgroup, demonstrated that, despite a number of unique features, SARS-CoV is most closely related to group 2 coronaviruses. Distant homologs of cellular RNA processing enzymes were identified in group 2 coronaviruses, with four of them being conserved in SARS-CoV. These newly recognized viral enzymes place the mechanism of coronavirus RNA synthesis in a completely new perspective. Furthermore, together with previously described viral enzymes, they will be important targets for the design of antiviral strategies aimed at controlling the further spread of SARS-CoV. © 2003 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79275
ISSN
2015 Impact Factor: 4.517
2015 SCImago Journal Rankings: 3.002
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSnijder, EJen_HK
dc.contributor.authorBredenbeek, PJen_HK
dc.contributor.authorDobbe, JCen_HK
dc.contributor.authorThiel, Ven_HK
dc.contributor.authorZiebuhr, Jen_HK
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorRozanov, Men_HK
dc.contributor.authorSpaan, WJMen_HK
dc.contributor.authorGorbalenya, AEen_HK
dc.date.accessioned2010-09-06T07:52:42Z-
dc.date.available2010-09-06T07:52:42Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Molecular Biology, 2003, v. 331 n. 5, p. 991-1004en_HK
dc.identifier.issn0022-2836en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79275-
dc.description.abstractThe genome organization and expression strategy of the newly identified severe acute respiratory syndrome coronavirus (SARS-CoV) were predicted using recently published genome sequences. Fourteen putative open reading frames were identified, 12 of which were predicted to be expressed from a nested set of eight subgenomic mRNAs. The synthesis of these mRNAs in SARS-CoV-infected cells was confirmed experimentally. The 4382- and 7073 amino acid residue SARS-CoV replicase polyproteins are predicted to be cleaved into 16 subunits by two viral proteinases (bringing the total number of SARS-CoV proteins to 28). A phylogenetic analysis of the replicase gene, using a distantly related torovirus as an outgroup, demonstrated that, despite a number of unique features, SARS-CoV is most closely related to group 2 coronaviruses. Distant homologs of cellular RNA processing enzymes were identified in group 2 coronaviruses, with four of them being conserved in SARS-CoV. These newly recognized viral enzymes place the mechanism of coronavirus RNA synthesis in a completely new perspective. Furthermore, together with previously described viral enzymes, they will be important targets for the design of antiviral strategies aimed at controlling the further spread of SARS-CoV. © 2003 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/jmben_HK
dc.relation.ispartofJournal of Molecular Biologyen_HK
dc.subjectGenome organizationen_HK
dc.subjectNidovirusen_HK
dc.subjectReplicaseen_HK
dc.subjectRNA processingen_HK
dc.subjectSubgenomic mRNA synthesisen_HK
dc.titleUnique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineageen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-2836&volume=331&issue=5&spage=991&epage=1004&date=2003&atitle=Unique+and+conserved+features+of+genome+and+proteome+of+SARS-coronavirus,+an+early+split-off+from+the+coronavirus+group+2+lineageen_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0022-2836(03)00865-9en_HK
dc.identifier.pmid12927536-
dc.identifier.scopuseid_2-s2.0-0042164218en_HK
dc.identifier.hkuros93273en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042164218&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume331en_HK
dc.identifier.issue5en_HK
dc.identifier.spage991en_HK
dc.identifier.epage1004en_HK
dc.identifier.isiWOS:000185036400004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.f10001014960-
dc.identifier.scopusauthoridSnijder, EJ=7006058325en_HK
dc.identifier.scopusauthoridBredenbeek, PJ=6603901441en_HK
dc.identifier.scopusauthoridDobbe, JC=6602684547en_HK
dc.identifier.scopusauthoridThiel, V=35238592100en_HK
dc.identifier.scopusauthoridZiebuhr, J=7003783935en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridRozanov, M=7003761371en_HK
dc.identifier.scopusauthoridSpaan, WJM=7007172944en_HK
dc.identifier.scopusauthoridGorbalenya, AE=7005626044en_HK

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