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Article: Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis

TitleConstruction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis
Authors
Issue Date2005
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1
Citation
Fems Microbiology Letters, 2005, v. 252 n. 1, p. 57-65 How to Cite?
AbstractAn Escherichia coli-Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli-L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79273
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.126
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorMa, SSLen_HK
dc.contributor.authorTeng, JLLen_HK
dc.contributor.authorLi, MWSen_HK
dc.contributor.authorKao, RYTen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:52:41Z-
dc.date.available2010-09-06T07:52:41Z-
dc.date.issued2005en_HK
dc.identifier.citationFems Microbiology Letters, 2005, v. 252 n. 1, p. 57-65en_HK
dc.identifier.issn0378-1097en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79273-
dc.description.abstractAn Escherichia coli-Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli-L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0378-1097&site=1en_HK
dc.relation.ispartofFEMS Microbiology Lettersen_HK
dc.rightsF E M S Microbiology Letters. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshEscherichia coli - genetics - metabolismen_HK
dc.subject.meshGastroenteritis - microbiologyen_HK
dc.subject.meshGene Expression Regulation, Bacterialen_HK
dc.subject.meshGenetic Vectorsen_HK
dc.subject.meshGlutathione Transferase - genetics - metabolismen_HK
dc.subject.meshGreen Fluorescent Proteins - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeisseriaceae - genetics - isolation & purification - metabolismen_HK
dc.subject.meshNeisseriaceae Infections - microbiologyen_HK
dc.subject.meshPlasmidsen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshTransformation, Geneticen_HK
dc.titleConstruction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-1097&volume=252&spage=57&epage=65&date=2005&atitle=Construction+of+an+inducible+expression+shuttle+vector+for+Laribacter+hongkongensis,+a+novel+bacterium+associated+with+gastroenteritisen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTeng, JLL:llteng@hku.hken_HK
dc.identifier.emailKao, RYT:rytkao@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTeng, JLL=rp00277en_HK
dc.identifier.authorityKao, RYT=rp00481en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.femsle.2005.08.026en_HK
dc.identifier.pmid16165318-
dc.identifier.scopuseid_2-s2.0-26844540494en_HK
dc.identifier.hkuros114681en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-26844540494&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume252en_HK
dc.identifier.issue1en_HK
dc.identifier.spage57en_HK
dc.identifier.epage65en_HK
dc.identifier.isiWOS:000232971800008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridMa, SSL=26424668200en_HK
dc.identifier.scopusauthoridTeng, JLL=7202560229en_HK
dc.identifier.scopusauthoridLi, MWS=8967745900en_HK
dc.identifier.scopusauthoridKao, RYT=7101675499en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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