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Article: Cloning and characterization of a chromosomal class C β-lactamase and its regulatory gene in Laribacter hongkongensis

TitleCloning and characterization of a chromosomal class C β-lactamase and its regulatory gene in Laribacter hongkongensis
Authors
Issue Date2005
PublisherAmerican Society for Microbiology.
Citation
Antimicrobial Agents And Chemotherapy, 2005, v. 49 n. 5, p. 1957-1964 How to Cite?
AbstractLaribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most β-lactams except the carbapenems. We describe the cloning and characterization of a novel chromosomal class C β-lactamase and its regulatory gene in L. hongkongensis. Two genes, ampC and ampR, were cloned by inserting restriction fragments of genomic DNA from L. hongkongensis strain HLHK5 into pBK-CMV to give the recombinant plasmid pBK-LHK-5. The ampR and ampC genes and their promoters were divergently oriented, with the ampR gene immediately upstream of the ampC gene and an intercistronic Lys-R motif, typical of inducible ampC-ampR regulatory systems. The deduced amino acid sequence of the cloned AmpC β-lactamase (pi 8.1) contained consensus motifs characteristic of class C β-lactamases but had identities no greater than 46% to known class C β-lactamases. The kinetic properties of this AmpC were also compatible with those of a class C β-lactamase. PCR of 20 clinical isolates of L. hongkongensis, including HLHK5, showed the presence of both ampC and ampR genes in all isolates. Southern hybridization suggested that the ampC gene of HLHKS was chromosomally encoded. Subcloning experiments showed that the expression of the ampC gene of HLHK5 was regulated by its ampR gene, which acts as a repressor. The β-lactamase characterized from strain HLHK5 was named LHK-5 (gene, blaLHK-5) and represents the first example of AmpC β-lactamase in the β subdivision of proteobacteria. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79251
ISSN
2014 Impact Factor: 4.476
2014 SCImago Journal Rankings: 2.005
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLi, MWSen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorYung, RWHen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:52:25Z-
dc.date.available2010-09-06T07:52:25Z-
dc.date.issued2005en_HK
dc.identifier.citationAntimicrobial Agents And Chemotherapy, 2005, v. 49 n. 5, p. 1957-1964en_HK
dc.identifier.issn0066-4804en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79251-
dc.description.abstractLaribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most β-lactams except the carbapenems. We describe the cloning and characterization of a novel chromosomal class C β-lactamase and its regulatory gene in L. hongkongensis. Two genes, ampC and ampR, were cloned by inserting restriction fragments of genomic DNA from L. hongkongensis strain HLHK5 into pBK-CMV to give the recombinant plasmid pBK-LHK-5. The ampR and ampC genes and their promoters were divergently oriented, with the ampR gene immediately upstream of the ampC gene and an intercistronic Lys-R motif, typical of inducible ampC-ampR regulatory systems. The deduced amino acid sequence of the cloned AmpC β-lactamase (pi 8.1) contained consensus motifs characteristic of class C β-lactamases but had identities no greater than 46% to known class C β-lactamases. The kinetic properties of this AmpC were also compatible with those of a class C β-lactamase. PCR of 20 clinical isolates of L. hongkongensis, including HLHK5, showed the presence of both ampC and ampR genes in all isolates. Southern hybridization suggested that the ampC gene of HLHKS was chromosomally encoded. Subcloning experiments showed that the expression of the ampC gene of HLHK5 was regulated by its ampR gene, which acts as a repressor. The β-lactamase characterized from strain HLHK5 was named LHK-5 (gene, blaLHK-5) and represents the first example of AmpC β-lactamase in the β subdivision of proteobacteria. Copyright © 2005, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofAntimicrobial Agents and Chemotherapyen_HK
dc.rightsAntimicrobial Agents and Chemotherapy. Copyright © American Society for Microbiology.en_HK
dc.subject.meshProteobacteria - enzymology - genetics-
dc.subject.meshBeta-Lactamases - chemistry - genetics-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshDNA, Bacterial - genetics-
dc.subject.meshDrug Resistance, Bacterial-
dc.titleCloning and characterization of a chromosomal class C β-lactamase and its regulatory gene in Laribacter hongkongensisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0066-4804&volume=49&spage=1957&epage=1964&date=2005&atitle=Cloning+and+Characterization+of+Chromosomal+Class+C+-Lactamase+and+Its+Regulatory+Gene+in+Laribacter+hongkongensis.en_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/AAC.49.5.1957-1964.2005en_HK
dc.identifier.pmid15855519-
dc.identifier.pmcidPMC1087626-
dc.identifier.scopuseid_2-s2.0-18244391437en_HK
dc.identifier.hkuros114670en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18244391437&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1957en_HK
dc.identifier.epage1964en_HK
dc.identifier.isiWOS:000228982600040-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLi, MWS=8967745900en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridYung, RWH=7005594277en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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