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Article: Cloning and characterization of a chromosomal class C β-lactamase and its regulatory gene in Laribacter hongkongensis
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TitleCloning and characterization of a chromosomal class C β-lactamase and its regulatory gene in Laribacter hongkongensis
 
AuthorsLau, SKP1
Ho, PL1
Li, MWS1
Tsoi, HW1
Yung, RWH2
Woo, PCY1
Yuen, KY1
 
Issue Date2005
 
PublisherAmerican Society for Microbiology.
 
CitationAntimicrobial Agents And Chemotherapy, 2005, v. 49 n. 5, p. 1957-1964 [How to Cite?]
DOI: http://dx.doi.org/10.1128/AAC.49.5.1957-1964.2005
 
AbstractLaribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most β-lactams except the carbapenems. We describe the cloning and characterization of a novel chromosomal class C β-lactamase and its regulatory gene in L. hongkongensis. Two genes, ampC and ampR, were cloned by inserting restriction fragments of genomic DNA from L. hongkongensis strain HLHK5 into pBK-CMV to give the recombinant plasmid pBK-LHK-5. The ampR and ampC genes and their promoters were divergently oriented, with the ampR gene immediately upstream of the ampC gene and an intercistronic Lys-R motif, typical of inducible ampC-ampR regulatory systems. The deduced amino acid sequence of the cloned AmpC β-lactamase (pi 8.1) contained consensus motifs characteristic of class C β-lactamases but had identities no greater than 46% to known class C β-lactamases. The kinetic properties of this AmpC were also compatible with those of a class C β-lactamase. PCR of 20 clinical isolates of L. hongkongensis, including HLHK5, showed the presence of both ampC and ampR genes in all isolates. Southern hybridization suggested that the ampC gene of HLHKS was chromosomally encoded. Subcloning experiments showed that the expression of the ampC gene of HLHK5 was regulated by its ampR gene, which acts as a repressor. The β-lactamase characterized from strain HLHK5 was named LHK-5 (gene, blaLHK-5) and represents the first example of AmpC β-lactamase in the β subdivision of proteobacteria. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
 
ISSN0066-4804
2013 Impact Factor: 4.451
 
DOIhttp://dx.doi.org/10.1128/AAC.49.5.1957-1964.2005
 
PubMed Central IDPMC1087626
 
ISI Accession Number IDWOS:000228982600040
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLau, SKP
 
dc.contributor.authorHo, PL
 
dc.contributor.authorLi, MWS
 
dc.contributor.authorTsoi, HW
 
dc.contributor.authorYung, RWH
 
dc.contributor.authorWoo, PCY
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2010-09-06T07:52:25Z
 
dc.date.available2010-09-06T07:52:25Z
 
dc.date.issued2005
 
dc.description.abstractLaribacter hongkongensis, a newly discovered bacterium recently shown to be associated with community-acquired gastroenteritis, is generally resistant to most β-lactams except the carbapenems. We describe the cloning and characterization of a novel chromosomal class C β-lactamase and its regulatory gene in L. hongkongensis. Two genes, ampC and ampR, were cloned by inserting restriction fragments of genomic DNA from L. hongkongensis strain HLHK5 into pBK-CMV to give the recombinant plasmid pBK-LHK-5. The ampR and ampC genes and their promoters were divergently oriented, with the ampR gene immediately upstream of the ampC gene and an intercistronic Lys-R motif, typical of inducible ampC-ampR regulatory systems. The deduced amino acid sequence of the cloned AmpC β-lactamase (pi 8.1) contained consensus motifs characteristic of class C β-lactamases but had identities no greater than 46% to known class C β-lactamases. The kinetic properties of this AmpC were also compatible with those of a class C β-lactamase. PCR of 20 clinical isolates of L. hongkongensis, including HLHK5, showed the presence of both ampC and ampR genes in all isolates. Southern hybridization suggested that the ampC gene of HLHKS was chromosomally encoded. Subcloning experiments showed that the expression of the ampC gene of HLHK5 was regulated by its ampR gene, which acts as a repressor. The β-lactamase characterized from strain HLHK5 was named LHK-5 (gene, blaLHK-5) and represents the first example of AmpC β-lactamase in the β subdivision of proteobacteria. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationAntimicrobial Agents And Chemotherapy, 2005, v. 49 n. 5, p. 1957-1964 [How to Cite?]
DOI: http://dx.doi.org/10.1128/AAC.49.5.1957-1964.2005
 
dc.identifier.doihttp://dx.doi.org/10.1128/AAC.49.5.1957-1964.2005
 
dc.identifier.epage1964
 
dc.identifier.hkuros114670
 
dc.identifier.isiWOS:000228982600040
 
dc.identifier.issn0066-4804
2013 Impact Factor: 4.451
 
dc.identifier.issue5
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC1087626
 
dc.identifier.pmid15855519
 
dc.identifier.scopuseid_2-s2.0-18244391437
 
dc.identifier.spage1957
 
dc.identifier.urihttp://hdl.handle.net/10722/79251
 
dc.identifier.volume49
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology.
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAntimicrobial Agents and Chemotherapy
 
dc.relation.referencesReferences in Scopus
 
dc.rightsAntimicrobial Agents and Chemotherapy. Copyright © American Society for Microbiology.
 
dc.subject.meshProteobacteria - enzymology - genetics
 
dc.subject.meshBeta-Lactamases - chemistry - genetics
 
dc.subject.meshAmino Acid Sequence
 
dc.subject.meshDNA, Bacterial - genetics
 
dc.subject.meshDrug Resistance, Bacterial
 
dc.titleCloning and characterization of a chromosomal class C β-lactamase and its regulatory gene in Laribacter hongkongensis
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Pamela Youde Nethersole Eastern Hospital