File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Exploring the Penicillium marneffei genome

TitleExploring the Penicillium marneffei genome
Authors
Issue Date2003
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htm
Citation
Archives Of Microbiology, 2003, v. 179 n. 5, p. 339-353 How to Cite?
AbstractPenicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies on thermal dimorphic switching and host-microbe interactions. Genomic analysis, therefore, could provide crucial information. Pulsed-field gel electrophoresis of genomic DNA of P. marneffei revealed three or more chromosomes (5.0, 4.0, and 2.2 Mb). Telomeric fingerprinting revealed 6-12 bands, suggesting that there were chromosomes of similar sizes. The genome size of P. marneffei was hence about 17.8-26.2 Mb. G+C content of the genome is 48:8 mol%. Random exploration of the genome of P. marneffei yielded 2303 random sequence tags (RSTs), corresponding to 9% of the genome, with 11.7, 6.3, and 17.4% of the RSTs having sequence similarity to yeast-specific sequences, non-yeast fungus sequences, and both (common sequences), respectively. Analysis of the RSTs revealed genes for information transfer (ribosomal protein genes, tRNA synthetase subunits, translation initiation, and elongation factors), metabolism, and compartmentalization, including several multi-drug-resistance protein genes and homologues of fluconazole-resistance gene. Furthermore, the presence of genes encoding pheromone homologues and ankyrin repeat-containing proteins of other fungi and algae strongly suggests the presence of a sexual stage that presumably exists in the environment.
Persistent Identifierhttp://hdl.handle.net/10722/79244
ISSN
2015 Impact Factor: 1.76
2015 SCImago Journal Rankings: 0.702
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorPascal, Gen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorGlaser, Pen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorKunst, Fen_HK
dc.contributor.authorCai, JJen_HK
dc.contributor.authorCheung, EYLen_HK
dc.contributor.authorMédigue, Cen_HK
dc.contributor.authorDanchin, Aen_HK
dc.date.accessioned2010-09-06T07:52:20Z-
dc.date.available2010-09-06T07:52:20Z-
dc.date.issued2003en_HK
dc.identifier.citationArchives Of Microbiology, 2003, v. 179 n. 5, p. 339-353en_HK
dc.identifier.issn0302-8933en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79244-
dc.description.abstractPenicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies on thermal dimorphic switching and host-microbe interactions. Genomic analysis, therefore, could provide crucial information. Pulsed-field gel electrophoresis of genomic DNA of P. marneffei revealed three or more chromosomes (5.0, 4.0, and 2.2 Mb). Telomeric fingerprinting revealed 6-12 bands, suggesting that there were chromosomes of similar sizes. The genome size of P. marneffei was hence about 17.8-26.2 Mb. G+C content of the genome is 48:8 mol%. Random exploration of the genome of P. marneffei yielded 2303 random sequence tags (RSTs), corresponding to 9% of the genome, with 11.7, 6.3, and 17.4% of the RSTs having sequence similarity to yeast-specific sequences, non-yeast fungus sequences, and both (common sequences), respectively. Analysis of the RSTs revealed genes for information transfer (ribosomal protein genes, tRNA synthetase subunits, translation initiation, and elongation factors), metabolism, and compartmentalization, including several multi-drug-resistance protein genes and homologues of fluconazole-resistance gene. Furthermore, the presence of genes encoding pheromone homologues and ankyrin repeat-containing proteins of other fungi and algae strongly suggests the presence of a sexual stage that presumably exists in the environment.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htmen_HK
dc.relation.ispartofArchives of Microbiologyen_HK
dc.subject.meshBase Compositionen_HK
dc.subject.meshDNA, Fungal - genetics - isolation & purificationen_HK
dc.subject.meshGenome, Fungalen_HK
dc.subject.meshPenicillium - classification - genetics - pathogenicity - physiologyen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshTelomere - geneticsen_HK
dc.titleExploring the Penicillium marneffei genomeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0302-8933&volume=179&spage=339&epage=353&date=2003&atitle=Exploring+the+Penicillium+marneffei+genomeen_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12640520-
dc.identifier.scopuseid_2-s2.0-0037615060en_HK
dc.identifier.hkuros80048en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037615060&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume179en_HK
dc.identifier.issue5en_HK
dc.identifier.spage339en_HK
dc.identifier.epage353en_HK
dc.identifier.isiWOS:000183048100005-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridPascal, G=8859539800en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridGlaser, P=7102878204en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridKunst, F=7003574115en_HK
dc.identifier.scopusauthoridCai, JJ=7403153560en_HK
dc.identifier.scopusauthoridCheung, EYL=36853286600en_HK
dc.identifier.scopusauthoridMédigue, C=7004599533en_HK
dc.identifier.scopusauthoridDanchin, A=7103235597en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats