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Article: The effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus

TitleThe effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus
Authors
Issue Date2005
PublisherChinese Medical Association [中華醫學會]. The Journal's web site is located at http://zhlxbx.periodicals.net.cn/
Citation
Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 2005, v. 26 n. 1, p. 22-24 How to Cite?
AbstractOBJECTIVE: To compare the 4 test kits on severe acute respiratory syndrome coronavirus (SARS-CoV) gene, antigen and antibody for early diagnose of SARS patients. METHODS: Three enzyme linked immunosorbent assay (ELISA) kits were used to detect SARS-CoV IgG, IgM and N protein and fluorescent polymerase chain reaction (F-PCR) kit was used to detect SARS-CoV RNA. RESULTS: In 162 serum samples, 90.2% (55/61) became N protein positive in 1 - 5 days and 92.8% (13/14) became positive IgM and IgG in 15 - 18 days after the onset of disease, respectively. On 82 gorgling samples, the positive rates of F-PCR were 56.3% (14/24) in 1 - 5 days and 71.4% (10/14) in 6 - 9 days after the onset. CONCLUSION: Other than F-PCR, N protein had good effect in the early detection on dubious patients which could lead to effective prevention and control of the epidemic.
Persistent Identifierhttp://hdl.handle.net/10722/79241
ISSN
2015 SCImago Journal Rankings: 0.191

 

DC FieldValueLanguage
dc.contributor.authorWang, Men_HK
dc.contributor.authorGao, Yen_HK
dc.contributor.authorZhou, DHen_HK
dc.contributor.authorWu, XWen_HK
dc.contributor.authorChen, XSen_HK
dc.contributor.authorDi, Ben_HK
dc.contributor.authorLiu, YFen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorDu, Len_HK
dc.contributor.authorXu, HFen_HK
dc.contributor.authorGu, Jen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorXu, JGen_HK
dc.date.accessioned2010-09-06T07:52:18Z-
dc.date.available2010-09-06T07:52:18Z-
dc.date.issued2005en_HK
dc.identifier.citationZhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 2005, v. 26 n. 1, p. 22-24en_HK
dc.identifier.issn0254-6450en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79241-
dc.description.abstractOBJECTIVE: To compare the 4 test kits on severe acute respiratory syndrome coronavirus (SARS-CoV) gene, antigen and antibody for early diagnose of SARS patients. METHODS: Three enzyme linked immunosorbent assay (ELISA) kits were used to detect SARS-CoV IgG, IgM and N protein and fluorescent polymerase chain reaction (F-PCR) kit was used to detect SARS-CoV RNA. RESULTS: In 162 serum samples, 90.2% (55/61) became N protein positive in 1 - 5 days and 92.8% (13/14) became positive IgM and IgG in 15 - 18 days after the onset of disease, respectively. On 82 gorgling samples, the positive rates of F-PCR were 56.3% (14/24) in 1 - 5 days and 71.4% (10/14) in 6 - 9 days after the onset. CONCLUSION: Other than F-PCR, N protein had good effect in the early detection on dubious patients which could lead to effective prevention and control of the epidemic.en_HK
dc.languageengen_HK
dc.publisherChinese Medical Association [中華醫學會]. The Journal's web site is located at http://zhlxbx.periodicals.net.cn/zh_HK
dc.relation.ispartofZhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhien_HK
dc.subject.meshAntibodies, Viral - blooden_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNucleocapsid Proteins - blooden_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshRNA, Viral - blooden_HK
dc.subject.meshReagent Kits, Diagnostic - standardsen_HK
dc.subject.meshSARS Virus - isolation & purificationen_HK
dc.subject.meshSevere Acute Respiratory Syndrome - diagnosis - virologyen_HK
dc.titleThe effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirusen_HK
dc.typeArticleen_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid15921587-
dc.identifier.scopuseid_2-s2.0-39049194836en_HK
dc.identifier.hkuros99122en_HK
dc.identifier.volume26en_HK
dc.identifier.issue1en_HK
dc.identifier.spage22en_HK
dc.identifier.epage24en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridWang, M=7406685232en_HK
dc.identifier.scopusauthoridGao, Y=7404706103en_HK
dc.identifier.scopusauthoridZhou, DH=7403395022en_HK
dc.identifier.scopusauthoridWu, XW=7407060805en_HK
dc.identifier.scopusauthoridChen, XS=9241082400en_HK
dc.identifier.scopusauthoridDi, B=6602190900en_HK
dc.identifier.scopusauthoridLiu, YF=12776732100en_HK
dc.identifier.scopusauthoridChen, F=26432493500en_HK
dc.identifier.scopusauthoridDu, L=35740266200en_HK
dc.identifier.scopusauthoridXu, HF=7407448207en_HK
dc.identifier.scopusauthoridGu, J=7403129372en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridXu, JG=8101558800en_HK

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