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Article: Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis

TitleDistribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis
Authors
Issue Date2008
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal Of Antimicrobial Chemotherapy, 2008, v. 61 n. 3, p. 488-497 How to Cite?
AbstractObjectives: Laribacter hongkongensis is a newly discovered bacterium associated with gastroenteritis and found in freshwater fish. Although isolates resistant to tetracycline have been described, their resistance mechanisms have not been studied. Patients and methods: We describe the distribution and molecular characterization of tetracycline resistance in 48 L. hongkongensis isolates from humans and fish. Results: Three human isolates and one fish isolate were resistant to tetracycline (MIC 128 mg/L) and doxycycline (MIC 8-16 mg/L) and had reduced susceptibility to minocycline (MIC 1-4 mg/L). A 3566 bp gene cluster, which contains tetR and tetA, was cloned from one of the tetracycline-resistant strains, HLHK5. While the flanking regions and 3′ end of the tetA of HLHK5 were identical to the corresponding regions of a tetC island in Chlamydia suis, the tetA gene was almost identical to that of transposon Tn 1721 and plasmids of Gram-negative bacteria, suggesting that the tetA/tetR of HLHK5 may have arisen from illegitimate recombination. PCR and DNA sequencing showed the presence of tetA in the other three tetracycline-resistant L. hongkongensis strains. Sequencing and characterization of a 15 665 bp plasmid, pHLHK22, from strain HLHK22 revealed the presence of a similar tetA/tetR gene cluster. This novel plasmid also confers tetracycline resistance when transformed to Escherichia coli and other L. hongkongensis isolates. Conclusions: Horizontal transfer of genes, especially through Tn 1721 and related plasmids, is likely an important mechanism for acquisition and dissemination of tetracycline resistance in L. hongkongensis. The present study is the first report on identification of tetA genes in bacteria of the Neisseriaceae family. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79220
ISSN
2015 Impact Factor: 4.919
2015 SCImago Journal Rankings: 2.157
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWong, GKMen_HK
dc.contributor.authorLi, MWSen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:52:03Z-
dc.date.available2010-09-06T07:52:03Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Antimicrobial Chemotherapy, 2008, v. 61 n. 3, p. 488-497en_HK
dc.identifier.issn0305-7453en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79220-
dc.description.abstractObjectives: Laribacter hongkongensis is a newly discovered bacterium associated with gastroenteritis and found in freshwater fish. Although isolates resistant to tetracycline have been described, their resistance mechanisms have not been studied. Patients and methods: We describe the distribution and molecular characterization of tetracycline resistance in 48 L. hongkongensis isolates from humans and fish. Results: Three human isolates and one fish isolate were resistant to tetracycline (MIC 128 mg/L) and doxycycline (MIC 8-16 mg/L) and had reduced susceptibility to minocycline (MIC 1-4 mg/L). A 3566 bp gene cluster, which contains tetR and tetA, was cloned from one of the tetracycline-resistant strains, HLHK5. While the flanking regions and 3′ end of the tetA of HLHK5 were identical to the corresponding regions of a tetC island in Chlamydia suis, the tetA gene was almost identical to that of transposon Tn 1721 and plasmids of Gram-negative bacteria, suggesting that the tetA/tetR of HLHK5 may have arisen from illegitimate recombination. PCR and DNA sequencing showed the presence of tetA in the other three tetracycline-resistant L. hongkongensis strains. Sequencing and characterization of a 15 665 bp plasmid, pHLHK22, from strain HLHK22 revealed the presence of a similar tetA/tetR gene cluster. This novel plasmid also confers tetracycline resistance when transformed to Escherichia coli and other L. hongkongensis isolates. Conclusions: Horizontal transfer of genes, especially through Tn 1721 and related plasmids, is likely an important mechanism for acquisition and dissemination of tetracycline resistance in L. hongkongensis. The present study is the first report on identification of tetA genes in bacteria of the Neisseriaceae family. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_HK
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntiporters - geneticsen_HK
dc.subject.meshBacterial Proteins - geneticsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCloning, Molecular - methodsen_HK
dc.subject.meshEscherichia coli Proteins - geneticsen_HK
dc.subject.meshFishes - microbiologyen_HK
dc.subject.meshGastroenteritis - genetics - microbiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicrobial Sensitivity Tests - methodsen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMultigene Family - geneticsen_HK
dc.subject.meshNeisseriaceae - genetics - isolation & purificationen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshTetracycline Resistance - geneticsen_HK
dc.subject.meshTransformation, Bacterial - geneticsen_HK
dc.titleDistribution and molecular characterization of tetracycline resistance in Laribacter hongkongensisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-7453&volume=61&spage=488&epage=497&date=2008&atitle=Distribution+and+molecular+characterization+of+tetracycline+resistance+in+Laribacter+hongkongensisen_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jac/dkm539en_HK
dc.identifier.pmid18227089-
dc.identifier.scopuseid_2-s2.0-40049101695en_HK
dc.identifier.hkuros149575en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40049101695&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume61en_HK
dc.identifier.issue3en_HK
dc.identifier.spage488en_HK
dc.identifier.epage497en_HK
dc.identifier.isiWOS:000253490800004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWong, GKM=9333006300en_HK
dc.identifier.scopusauthoridLi, MWS=8967745900en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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