File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Detection of mutations in RNA polymerase beta subunit gene encoding resistance to rifampin in Mycobacterium tuberculosis by DNA microarray

TitleDetection of mutations in RNA polymerase beta subunit gene encoding resistance to rifampin in Mycobacterium tuberculosis by DNA microarray
Authors
KeywordsDifferential hybridization
DNA microarray
Mycobacterium tuberculosis
Rifampin resistance
RNA polymerase beta subunit (rpoB) gene
Issue Date2005
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00032719.asp
Citation
Analytical Letters, 2005, v. 38 n. 13, p. 2117-2134 How to Cite?
AbstractRapid and efficient diagnosis is essential in the management of drug-resistant tuberculosis. A DNA microarray technique based on differential hybridization method was described in the present study for detecting mutations in the RNA polymerase beta subunit (rpoB) gene of Mycobacterium tuberculosis (M. tuberculosis) cultures and in clinical specimens. The mutations in rpoB confer resistance to rifampin, an important first-line antituberculosis drug. The differential hybridization approach was mainly based on the effect of a single base mismatch on the melting temperature of the hybridized DNA; therefore, any point mutation of rpoB gene resulting in the rifampin resistance can be detected efficiently. The development of the DNA microarray involves the design of dozens of oligonucleotide probes for identifying rifampin-resistant and -sensitive strains. The method comprises isolating genomic DNA from the samples containing M. tuberculosis cells, amplifying rpoB gene coding sequence to produce fluorescently labelled product, and hybridization with the oligonucleotide arrays. The results demonstrated the capability of DNA microarray to provide important clinically relevant information about the rpoB gene of mycobacterial organisms. The DNA microarray offers a reliable diagnostic test for rapidly detecting multidrug resistance caused by gene mutations of mycobacteria. Copyright © Taylor & Francis, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/79216
ISSN
2015 Impact Factor: 1.088
2015 SCImago Journal Rankings: 0.368
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Men_HK
dc.contributor.authorPui, YTen_HK
dc.contributor.authorLi, CWen_HK
dc.contributor.authorHok, SWen_HK
dc.contributor.authorZhao, Jen_HK
dc.contributor.authorYam, WCen_HK
dc.date.accessioned2010-09-06T07:52:00Z-
dc.date.available2010-09-06T07:52:00Z-
dc.date.issued2005en_HK
dc.identifier.citationAnalytical Letters, 2005, v. 38 n. 13, p. 2117-2134en_HK
dc.identifier.issn0003-2719en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79216-
dc.description.abstractRapid and efficient diagnosis is essential in the management of drug-resistant tuberculosis. A DNA microarray technique based on differential hybridization method was described in the present study for detecting mutations in the RNA polymerase beta subunit (rpoB) gene of Mycobacterium tuberculosis (M. tuberculosis) cultures and in clinical specimens. The mutations in rpoB confer resistance to rifampin, an important first-line antituberculosis drug. The differential hybridization approach was mainly based on the effect of a single base mismatch on the melting temperature of the hybridized DNA; therefore, any point mutation of rpoB gene resulting in the rifampin resistance can be detected efficiently. The development of the DNA microarray involves the design of dozens of oligonucleotide probes for identifying rifampin-resistant and -sensitive strains. The method comprises isolating genomic DNA from the samples containing M. tuberculosis cells, amplifying rpoB gene coding sequence to produce fluorescently labelled product, and hybridization with the oligonucleotide arrays. The results demonstrated the capability of DNA microarray to provide important clinically relevant information about the rpoB gene of mycobacterial organisms. The DNA microarray offers a reliable diagnostic test for rapidly detecting multidrug resistance caused by gene mutations of mycobacteria. Copyright © Taylor & Francis, Inc.en_HK
dc.languageengen_HK
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00032719.aspen_HK
dc.relation.ispartofAnalytical Lettersen_HK
dc.subjectDifferential hybridizationen_HK
dc.subjectDNA microarrayen_HK
dc.subjectMycobacterium tuberculosisen_HK
dc.subjectRifampin resistanceen_HK
dc.subjectRNA polymerase beta subunit (rpoB) geneen_HK
dc.titleDetection of mutations in RNA polymerase beta subunit gene encoding resistance to rifampin in Mycobacterium tuberculosis by DNA microarrayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-2719&volume=38 &issue=13&spage=2117&epage=2134&date=2005&atitle=Detection+of+mutations+in+RNA+polymerase+beta+subunit+gene+encoding+resistance+to+rifampin+in+Mycobacterium+tuberculosis+by+DNA+microarray++en_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/00032710500260845en_HK
dc.identifier.scopuseid_2-s2.0-27544443743en_HK
dc.identifier.hkuros137498en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27544443743&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue13en_HK
dc.identifier.spage2117en_HK
dc.identifier.epage2134en_HK
dc.identifier.isiWOS:000232699400009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYang, M=7404925734en_HK
dc.identifier.scopusauthoridPui, YT=8972683500en_HK
dc.identifier.scopusauthoridLi, CW=8972683600en_HK
dc.identifier.scopusauthoridHok, SW=8972683700en_HK
dc.identifier.scopusauthoridZhao, J=7410312739en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats