File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Cell-wall-deficient bacteria and culture-negative febrile episodes in bone-marrow-transplant recipients

TitleCell-wall-deficient bacteria and culture-negative febrile episodes in bone-marrow-transplant recipients
Authors
Issue Date2001
PublisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lancet
Citation
Lancet, 2001, v. 357 n. 9257, p. 675-679 How to Cite?
AbstractBackground: Although about 75-80% of neutropenic fevers are thought to be caused by infections, a causal organism can be confirmed microbiologically or suspected clinically in only 30-50%, and even fewer of these cases (16%) have a documented bacteraemia. The cause of neutropenic fever in the remaining cases remains elusive. We investigate the role of cell-wall-deficient bacteria in bone-marrow transplantation (BMT). Methods: Blood cultures were obtained from bone-marrow-transplant recipients and were inoculated into an aerobic bottle with resin, an anaerobic bottle, and a bottle to isolate cell-wall deficient bacteria. When growth was detected in the blood-culture bottle for cell-wall-deficient bacteria, 20 μL of the broth was subcultured and the isolate identified by standard biochemical methods. The microbiological findings were correlated with the clinical characteristics of the patients. Findings: From the 86 BMT recipients enrolled into the study, 798 sets of blood cultures were sent for laboratory analysis. 55 blood cultures were positive in the aerobic bottle, and the same isolates were also detected in 52 blood-culture bottles for cell-wall-deficient forms. For 20 sets of blood cultures, the isolates were detected only in the bottle for cell-wall-deficient forms. Blood for 13 (65%) of these 20 sets were sampled in the pre-engraftment neutropenic period, three (15%) during conditioning when the absolute neutrophil count was above 0.5 × 10 9/L, and four (20%) in the post-engraftment period. 17 (85%) of the isolates were gram positive: four (20%) were Staphylococcus spp and ten (50%) were Bacillus spp. Antibiotic treatment was successful in 19 (95%) of 20 episodes. Interpretation: Bacteraemia due to cell-wall-deficient forms causes a significant proportion of so-called culture-negative febrile episodes in BMT recipients.
Persistent Identifierhttp://hdl.handle.net/10722/79211
ISSN
2015 Impact Factor: 44.002
2015 SCImago Journal Rankings: 14.638
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorLum, PNLen_HK
dc.contributor.authorHui, WTen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:51:56Z-
dc.date.available2010-09-06T07:51:56Z-
dc.date.issued2001en_HK
dc.identifier.citationLancet, 2001, v. 357 n. 9257, p. 675-679en_HK
dc.identifier.issn0140-6736en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79211-
dc.description.abstractBackground: Although about 75-80% of neutropenic fevers are thought to be caused by infections, a causal organism can be confirmed microbiologically or suspected clinically in only 30-50%, and even fewer of these cases (16%) have a documented bacteraemia. The cause of neutropenic fever in the remaining cases remains elusive. We investigate the role of cell-wall-deficient bacteria in bone-marrow transplantation (BMT). Methods: Blood cultures were obtained from bone-marrow-transplant recipients and were inoculated into an aerobic bottle with resin, an anaerobic bottle, and a bottle to isolate cell-wall deficient bacteria. When growth was detected in the blood-culture bottle for cell-wall-deficient bacteria, 20 μL of the broth was subcultured and the isolate identified by standard biochemical methods. The microbiological findings were correlated with the clinical characteristics of the patients. Findings: From the 86 BMT recipients enrolled into the study, 798 sets of blood cultures were sent for laboratory analysis. 55 blood cultures were positive in the aerobic bottle, and the same isolates were also detected in 52 blood-culture bottles for cell-wall-deficient forms. For 20 sets of blood cultures, the isolates were detected only in the bottle for cell-wall-deficient forms. Blood for 13 (65%) of these 20 sets were sampled in the pre-engraftment neutropenic period, three (15%) during conditioning when the absolute neutrophil count was above 0.5 × 10 9/L, and four (20%) in the post-engraftment period. 17 (85%) of the isolates were gram positive: four (20%) were Staphylococcus spp and ten (50%) were Bacillus spp. Antibiotic treatment was successful in 19 (95%) of 20 episodes. Interpretation: Bacteraemia due to cell-wall-deficient forms causes a significant proportion of so-called culture-negative febrile episodes in BMT recipients.en_HK
dc.languageengen_HK
dc.publisherThe Lancet Publishing Group. The Journal's web site is located at http://www.elsevier.com/locate/lanceten_HK
dc.relation.ispartofLanceten_HK
dc.subject.meshAnalysis of Varianceen_HK
dc.subject.meshBacteremia - diagnosis - microbiologyen_HK
dc.subject.meshBone Marrow Transplantation - adverse effectsen_HK
dc.subject.meshCell Wallen_HK
dc.subject.meshCulture Mediaen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFever - microbiologyen_HK
dc.subject.meshGram-Negative Bacteria - cytology - isolation & purificationen_HK
dc.subject.meshGram-Positive Bacteria - cytology - isolation & purificationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNeutropenia - complicationsen_HK
dc.titleCell-wall-deficient bacteria and culture-negative febrile episodes in bone-marrow-transplant recipientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0140-6736&volume=357&spage=675&epage=679&date=2001&atitle=Cell-wall-deficient+bacteria+and+culture-negative+febrile+episodes+in+bone-marrow-transplant+recipientsen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0140-6736(00)04131-3en_HK
dc.identifier.pmid11247551-
dc.identifier.scopuseid_2-s2.0-0035799122en_HK
dc.identifier.hkuros61953en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035799122&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume357en_HK
dc.identifier.issue9257en_HK
dc.identifier.spage675en_HK
dc.identifier.epage679en_HK
dc.identifier.isiWOS:000167282400012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridLum, PNL=7005247001en_HK
dc.identifier.scopusauthoridHui, WT=7103196480en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats