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Article: Characterization of a novel insertion sequence, ISBp1, in Burkholderia pseudomallei

TitleCharacterization of a novel insertion sequence, ISBp1, in Burkholderia pseudomallei
Authors
Issue Date2002
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htm
Citation
Archives Of Microbiology, 2002, v. 177 n. 3, p. 267-273 How to Cite?
AbstractDuring screening for antigenic proteins in Burkholderia pseudomallei, a novel insertion sequence, ISBp1, was found by sequence similarity searches. ISBp1 contains two overlapping ORFs of 261 bp (orfA) and 852 bp (orfB), encoding 87 and 284 amino acid residues, respectively, and an imperfect inverted repeat. The putative protein encoded by orfA (OrfA) is similar to the OrfA in insertion sequences of the IS3 family in other bacteria, showing 49% and 76% amino acid identity and similarity, respectively, with the transposase encoded by ISD1 of Desulfovibrio vulgaris vulgaris. The putative protein encoded by orfB (OrfB) is similar to the OrfB in insertion sequences of the IS3 family in other bacteria, showing 43% and 62% amino acid identity and similarity, respectively, with the transposase encoded by IS1222 of Enterobacter agglomerans. Sequence analysis of OrfA showed the presence of an α-helix-turn-α-helix motif, as well as the putative leucine zipper at its 3′ end, for possible DNA binding to the terminal inverted repeats. Sequence analysis of OrfB showed the presence of a DDE motif of aspartic acid, aspartic acid, and glutamic acid, a highly conserved motif present in OrfB of other members of the IS3 family. Furthermore, several other conserved amino acid residues, including the arginine residue located seven amino acids downstream from the glutamic acid residue, were observed. PCR amplification of the ISBp1 gene showed a specific band in 65% of the 26 B. pseudomallei strains tested. Southern blot hybridization after XhoI or SacI digestion showed nine different patterns of hybridization. The number of copies of ISBp1 in those strains that possessed the insertion sequence ranged from three to 12. Using several insertion sequences and a combination of insertion-sequence-based and non-insertion-sequence-based methods such as ribotyping will probably increase the discriminatory power of molecular typing in B. pseudomallei.
Persistent Identifierhttp://hdl.handle.net/10722/79205
ISSN
2015 Impact Factor: 1.76
2015 SCImago Journal Rankings: 0.702
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLeung, PKLen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorChan, BYLen_HK
dc.contributor.authorQue, TLen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:51:52Z-
dc.date.available2010-09-06T07:51:52Z-
dc.date.issued2002en_HK
dc.identifier.citationArchives Of Microbiology, 2002, v. 177 n. 3, p. 267-273en_HK
dc.identifier.issn0302-8933en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79205-
dc.description.abstractDuring screening for antigenic proteins in Burkholderia pseudomallei, a novel insertion sequence, ISBp1, was found by sequence similarity searches. ISBp1 contains two overlapping ORFs of 261 bp (orfA) and 852 bp (orfB), encoding 87 and 284 amino acid residues, respectively, and an imperfect inverted repeat. The putative protein encoded by orfA (OrfA) is similar to the OrfA in insertion sequences of the IS3 family in other bacteria, showing 49% and 76% amino acid identity and similarity, respectively, with the transposase encoded by ISD1 of Desulfovibrio vulgaris vulgaris. The putative protein encoded by orfB (OrfB) is similar to the OrfB in insertion sequences of the IS3 family in other bacteria, showing 43% and 62% amino acid identity and similarity, respectively, with the transposase encoded by IS1222 of Enterobacter agglomerans. Sequence analysis of OrfA showed the presence of an α-helix-turn-α-helix motif, as well as the putative leucine zipper at its 3′ end, for possible DNA binding to the terminal inverted repeats. Sequence analysis of OrfB showed the presence of a DDE motif of aspartic acid, aspartic acid, and glutamic acid, a highly conserved motif present in OrfB of other members of the IS3 family. Furthermore, several other conserved amino acid residues, including the arginine residue located seven amino acids downstream from the glutamic acid residue, were observed. PCR amplification of the ISBp1 gene showed a specific band in 65% of the 26 B. pseudomallei strains tested. Southern blot hybridization after XhoI or SacI digestion showed nine different patterns of hybridization. The number of copies of ISBp1 in those strains that possessed the insertion sequence ranged from three to 12. Using several insertion sequences and a combination of insertion-sequence-based and non-insertion-sequence-based methods such as ribotyping will probably increase the discriminatory power of molecular typing in B. pseudomallei.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00203/index.htmen_HK
dc.relation.ispartofArchives of Microbiologyen_HK
dc.subject.meshBacterial Proteins - geneticsen_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshBurkholderia pseudomallei - chemistry - classification - geneticsen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDNA Transposable Elements - geneticsen_HK
dc.subject.meshDNA, Bacterial - geneticsen_HK
dc.subject.meshEscherichia coli Proteinsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.subject.meshSequence Homology, Nucleic Aciden_HK
dc.titleCharacterization of a novel insertion sequence, ISBp1, in Burkholderia pseudomalleien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0302-8933&volume=177&spage=267&epage=273&date=2002&atitle=Characterization+of+a+novel+insertion+sequence,+ISBp1,+in+Burkholderia+pseudomalleien_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00203-001-0389-8en_HK
dc.identifier.pmid11907683-
dc.identifier.scopuseid_2-s2.0-0036185907en_HK
dc.identifier.hkuros74420en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036185907&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume177en_HK
dc.identifier.issue3en_HK
dc.identifier.spage267en_HK
dc.identifier.epage273en_HK
dc.identifier.isiWOS:000174674400009-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLeung, PKL=55085129100en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridChan, BYL=7201530670en_HK
dc.identifier.scopusauthoridQue, TL=7003786628en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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