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Article: Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

TitlePlasmid profile and construction of a small shuttle vector in Laribacter hongkongensis
Authors
Issue Date2007
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492
Citation
Biotechnology Letters, 2007, v. 29 n. 10, p. 1575-1582 How to Cite?
AbstractAmong 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies. © 2007 Springer Science+Business Media B.V.
Persistent Identifierhttp://hdl.handle.net/10722/79191
ISSN
2015 Impact Factor: 1.639
2015 SCImago Journal Rankings: 0.591
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorMa, SSLen_HK
dc.contributor.authorTeng, JLLen_HK
dc.contributor.authorLi, MWSen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:51:42Z-
dc.date.available2010-09-06T07:51:42Z-
dc.date.issued2007en_HK
dc.identifier.citationBiotechnology Letters, 2007, v. 29 n. 10, p. 1575-1582en_HK
dc.identifier.issn0141-5492en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79191-
dc.description.abstractAmong 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies. © 2007 Springer Science+Business Media B.V.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492en_HK
dc.relation.ispartofBiotechnology Lettersen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshDNA Helicases - geneticsen_HK
dc.subject.meshDNA-Binding Proteins - geneticsen_HK
dc.subject.meshEscherichia coli - geneticsen_HK
dc.subject.meshGenetic Vectors - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeisseriaceae - geneticsen_HK
dc.subject.meshOpen Reading Frames - geneticsen_HK
dc.subject.meshPlasmids - geneticsen_HK
dc.subject.meshReplication Origin - geneticsen_HK
dc.subject.meshTrans-Activators - geneticsen_HK
dc.subject.meshTransformation, Bacterialen_HK
dc.titlePlasmid profile and construction of a small shuttle vector in Laribacter hongkongensisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-5492&volume=29&spage=1575&epage=1582&date=2007&atitle=Plasmid+profile+and+construction+of+a+small+shuttle+vector+in+Laribacter+hongkongensisen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTeng, JLL:llteng@hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTeng, JLL=rp00277en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10529-007-9447-xen_HK
dc.identifier.pmid17609856-
dc.identifier.scopuseid_2-s2.0-34548119182en_HK
dc.identifier.hkuros134251en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548119182&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1575en_HK
dc.identifier.epage1582en_HK
dc.identifier.isiWOS:000248940500020-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridMa, SSL=26424668200en_HK
dc.identifier.scopusauthoridTeng, JLL=7202560229en_HK
dc.identifier.scopusauthoridLi, MWS=8967745900en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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