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Article: Contribution of C/EBP proteins to Epstein-Barr virus lytic gene expression and replication in epithelial cells

TitleContribution of C/EBP proteins to Epstein-Barr virus lytic gene expression and replication in epithelial cells
Authors
Issue Date2006
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2006, v. 80 n. 3, p. 1098-1109 How to Cite?
AbstractThe contribution of C/EBP proteins to Epstein-Barr virus (EBV) lytic gene expression and replication in epithelial cells was examined. Nasopharyngeal carcinoma cell lines constitutively expressed C/EBPβ and had limited C/EBPα expression, while the AGS gastric cancer cell line expressed significant levels of both C/EBPα and C/EBPβ. Induction of the lytic cycle in EBV-positive AGS/BX1 cells with phorbol ester and sodium butyrate treatment led to a transient stimulation of C/EBPβ expression and a prolonged increase in C/EBPα expression. In AGS/BX1 cells, endogenous C/EBPα and C/EBPβ proteins were detected associated with the ZTA and oriLyt promoters but not the RTA promoter. Electrophoretic mobility shift assays confirmed binding of C/EBP proteins to multiple sites in the ZTA and oriLyt promoters. The response of these promoters in reporter assays to transfected C/EBPα and C/EBPβ proteins was consistent with the promoter binding assays and emphasized the relative importance of C/EBPs for activation of the ZTA promoter. Mutation of the oriLyt promoter proximal C/EBP site had little effect on ZTA activation of the promoter in a reporter assay. However, this mutation impaired oriLyt DNA replication, suggesting a separate replication-specific contribution for C/EBP proteins. Finally, the overall importance of C/EBP proteins for lytic gene expression was demonstrated using CHOP10 to antagonize C/EBP DNA binding activity. Introduction of CHOP10 significantly impaired induction of the ZTA, RTA, and BMRF1 proteins in chemically treated AGS/BX1 cells. Thus, C/EBPβ and C/EBPα expression are associated with lytic induction in AGS cells, and expression of C/EBP proteins in epithelial cells may contribute to the tendency of these cells to exhibit constitutive low-level ZTA promoter activity. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79182
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, Jen_HK
dc.contributor.authorLiao, Gen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorWu, FYen_HK
dc.contributor.authorHuttFletcher, Len_HK
dc.contributor.authorHayward, GSen_HK
dc.contributor.authorHayward, SDen_HK
dc.date.accessioned2010-09-06T07:51:35Z-
dc.date.available2010-09-06T07:51:35Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Virology, 2006, v. 80 n. 3, p. 1098-1109en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/79182-
dc.description.abstractThe contribution of C/EBP proteins to Epstein-Barr virus (EBV) lytic gene expression and replication in epithelial cells was examined. Nasopharyngeal carcinoma cell lines constitutively expressed C/EBPβ and had limited C/EBPα expression, while the AGS gastric cancer cell line expressed significant levels of both C/EBPα and C/EBPβ. Induction of the lytic cycle in EBV-positive AGS/BX1 cells with phorbol ester and sodium butyrate treatment led to a transient stimulation of C/EBPβ expression and a prolonged increase in C/EBPα expression. In AGS/BX1 cells, endogenous C/EBPα and C/EBPβ proteins were detected associated with the ZTA and oriLyt promoters but not the RTA promoter. Electrophoretic mobility shift assays confirmed binding of C/EBP proteins to multiple sites in the ZTA and oriLyt promoters. The response of these promoters in reporter assays to transfected C/EBPα and C/EBPβ proteins was consistent with the promoter binding assays and emphasized the relative importance of C/EBPs for activation of the ZTA promoter. Mutation of the oriLyt promoter proximal C/EBP site had little effect on ZTA activation of the promoter in a reporter assay. However, this mutation impaired oriLyt DNA replication, suggesting a separate replication-specific contribution for C/EBP proteins. Finally, the overall importance of C/EBP proteins for lytic gene expression was demonstrated using CHOP10 to antagonize C/EBP DNA binding activity. Introduction of CHOP10 significantly impaired induction of the ZTA, RTA, and BMRF1 proteins in chemically treated AGS/BX1 cells. Thus, C/EBPβ and C/EBPα expression are associated with lytic induction in AGS cells, and expression of C/EBP proteins in epithelial cells may contribute to the tendency of these cells to exhibit constitutive low-level ZTA promoter activity. Copyright © 2006, American Society for Microbiology. All Rights Reserved.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_HK
dc.subject.meshAntigens, Viral - biosynthesis - geneticsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCCAAT-Enhancer-Binding Proteins - genetics - metabolismen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshDNA, Viral - geneticsen_HK
dc.subject.meshDNA-Binding Proteins - biosynthesis - geneticsen_HK
dc.subject.meshEpithelial Cells - metabolism - virologyen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshGenes, Viralen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHerpesvirus 4, Human - genetics - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmediate-Early Proteins - biosynthesis - geneticsen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshTrans-Activators - biosynthesis - geneticsen_HK
dc.subject.meshViral Proteins - biosynthesis - geneticsen_HK
dc.subject.meshVirus Replicationen_HK
dc.titleContribution of C/EBP proteins to Epstein-Barr virus lytic gene expression and replication in epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=80&issue=3&spage=1098&epage=1109&date=2006&atitle=Contribution+of+C/EBP+proteins+to+Epstein-Barr+virus+lytic+gene+expression+and+replication+in+epithelial+cells.en_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JVI.80.3.1098-1109.2006en_HK
dc.identifier.pmid16414987-
dc.identifier.scopuseid_2-s2.0-31144444025en_HK
dc.identifier.hkuros138145en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-31144444025&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume80en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1098en_HK
dc.identifier.epage1109en_HK
dc.identifier.isiWOS:000234871400004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHuang, J=7408108445en_HK
dc.identifier.scopusauthoridLiao, G=7102949054en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridWu, FY=36991458700en_HK
dc.identifier.scopusauthoridHuttFletcher, L=7004909823en_HK
dc.identifier.scopusauthoridHayward, GS=7101602499en_HK
dc.identifier.scopusauthoridHayward, SD=7102776214en_HK

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