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Article: High expression of PSM-E correlated with tumor grade in prostate cancer: A new alternatively spliced variant of prostate-specific membrane antigen

TitleHigh expression of PSM-E correlated with tumor grade in prostate cancer: A new alternatively spliced variant of prostate-specific membrane antigen
Authors
Issue Date2007
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 2007, v. 67 n. 16, p. 1791-1800 How to Cite?
AbstractBACKGROUND. Prostate-specific membrane antigen (PSMA) overexpressed in prostate cancer (PCa) has been targeted for therapy and diagnosis of PCa. In the current study, PSMA cDNA was cloned from PCa tissue by RT-PCR. After sequencing, a new spliced variant of PSMA (PSM-E) was discovered and its specificity in PCa was evaluated. METHODS. PSM-E and PSMA mRNA were measured in LNCaP, PC-3 and prostate or nonprostatic malignancies. Following transfection of PC-3 with PSM-E cDNA in the pcDNA3.0 vector, PSM-E expression was measured by immunofluorescence and Western-blot. PSM-E and PSMA mRNA levels were quantified by a real-time PCR assay in normal prostate (n = 7), benign prostatic hyperplasia (BPH) (n = 22) and PCa (n = 41). The correlation between their levels and tumor grade was analyzed. RESULTS. PSM-E cDNA is identical to PSMA except for a 97-nucleotide region and a 93-nucleotide region. PSM-E and PSMA mRNA were detected in PCa and LNCaP, not in PC-3; PSMA could be detected in some nonprostatic tumors whereas PSM-E not. The expression of PSM-E protein was detected in transfected cells. Significant difference of PSM-E mRNA levels was observed among normal prostate, BPH and PCa (P < 0.001), and PSM-E levels increased with increasing Gleason score (r = 0.514, P < 0.001). PSMA mRNA levels were higher in BPH and PCa than in normal prostate (P < 0.001), but no difference between BPH and PCa, no significant correlation was observed between PSMA levels and Gleason score (r = 0.229, P = 0.057). CONCLUSIONS. PSM-E may be a potential prognostic indicator for PCa progression and may be a new target antigen for therapy of PCa. © 2007 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/79181
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCao, KYen_HK
dc.contributor.authorMao, XPen_HK
dc.contributor.authorWang, DHen_HK
dc.contributor.authorXu, Len_HK
dc.contributor.authorYuan, GQen_HK
dc.contributor.authorDai, SQen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorQiu, SPen_HK
dc.date.accessioned2010-09-06T07:51:35Z-
dc.date.available2010-09-06T07:51:35Z-
dc.date.issued2007en_HK
dc.identifier.citationProstate, 2007, v. 67 n. 16, p. 1791-1800en_HK
dc.identifier.issn0270-4137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79181-
dc.description.abstractBACKGROUND. Prostate-specific membrane antigen (PSMA) overexpressed in prostate cancer (PCa) has been targeted for therapy and diagnosis of PCa. In the current study, PSMA cDNA was cloned from PCa tissue by RT-PCR. After sequencing, a new spliced variant of PSMA (PSM-E) was discovered and its specificity in PCa was evaluated. METHODS. PSM-E and PSMA mRNA were measured in LNCaP, PC-3 and prostate or nonprostatic malignancies. Following transfection of PC-3 with PSM-E cDNA in the pcDNA3.0 vector, PSM-E expression was measured by immunofluorescence and Western-blot. PSM-E and PSMA mRNA levels were quantified by a real-time PCR assay in normal prostate (n = 7), benign prostatic hyperplasia (BPH) (n = 22) and PCa (n = 41). The correlation between their levels and tumor grade was analyzed. RESULTS. PSM-E cDNA is identical to PSMA except for a 97-nucleotide region and a 93-nucleotide region. PSM-E and PSMA mRNA were detected in PCa and LNCaP, not in PC-3; PSMA could be detected in some nonprostatic tumors whereas PSM-E not. The expression of PSM-E protein was detected in transfected cells. Significant difference of PSM-E mRNA levels was observed among normal prostate, BPH and PCa (P < 0.001), and PSM-E levels increased with increasing Gleason score (r = 0.514, P < 0.001). PSMA mRNA levels were higher in BPH and PCa than in normal prostate (P < 0.001), but no difference between BPH and PCa, no significant correlation was observed between PSMA levels and Gleason score (r = 0.229, P = 0.057). CONCLUSIONS. PSM-E may be a potential prognostic indicator for PCa progression and may be a new target antigen for therapy of PCa. © 2007 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_HK
dc.relation.ispartofProstateen_HK
dc.rightsThe Prostate. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshAlternative Splicingen_HK
dc.subject.meshAntigens, Surface - biosynthesis - geneticsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshGlutamate Carboxypeptidase II - biosynthesis - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicroscopy, Fluorescenceen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshProstatic Neoplasms - genetics - metabolismen_HK
dc.subject.meshProtein Isoformsen_HK
dc.subject.meshRNA, Messenger - biosynthesis - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshStatistics, Nonparametricen_HK
dc.subject.meshTransfectionen_HK
dc.titleHigh expression of PSM-E correlated with tumor grade in prostate cancer: A new alternatively spliced variant of prostate-specific membrane antigenen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-4137&volume=67&issue=16&spage=1791&epage=1800&date=2007&atitle=High+Expression+of+PSM-E+Correlated+with+Tumor+Grade+in+Prostate+Cancer:+A+New+Alternatively+Spliced+Variant+of+Prostate-Specific+Membrane+Antigen.en_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pros.20664en_HK
dc.identifier.pmid17929272-
dc.identifier.scopuseid_2-s2.0-36849069530en_HK
dc.identifier.hkuros142910en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36849069530&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume67en_HK
dc.identifier.issue16en_HK
dc.identifier.spage1791en_HK
dc.identifier.epage1800en_HK
dc.identifier.isiWOS:000251572300008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCao, KY=15845100900en_HK
dc.identifier.scopusauthoridMao, XP=36727723500en_HK
dc.identifier.scopusauthoridWang, DH=14822799200en_HK
dc.identifier.scopusauthoridXu, L=7404744449en_HK
dc.identifier.scopusauthoridYuan, GQ=23037678100en_HK
dc.identifier.scopusauthoridDai, SQ=23034052100en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridQiu, SP=8305855300en_HK

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