File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Detection of cell wall galactomannoprotein Afmp1p in culture supernatants of Aspergillus fumigatus and in sera of aspergillosis patients

TitleDetection of cell wall galactomannoprotein Afmp1p in culture supernatants of Aspergillus fumigatus and in sera of aspergillosis patients
Authors
Issue Date2002
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2002, v. 40 n. 11, p. 4382-4387 How to Cite?
AbstractMannoproteins are important and abundant structural components of fungal cell walls. The AFMP1 gene encodes a cell wall galactomannoprotein of Aspergillusfumigatus. In the present study, we show that Afmplp is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Afmplp was capable of detecting this protein from the cell culture supernatant of A. fumigatus. The anti-Afmp1p antibody is specific since it fails to react with any protein from lysates of Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Penicillium marneffei, Candida albicans, Cryptococcus neoformans, Blastomyces dermatitidis, and Histoplasma capsulatum by Western blotting. In addition, this Afmp1p antigen-based ELISA is also specific for 4. fumigatus since the cell culture supernatants of the other eight fungi gave negative results. Finally, a clinical evaluation of sera from invasive aspergillosis patients indicates that 8 of 15 (53%) patients are Afmplp antigen test positive. Furthermore, an Afmplp antibody test was performed with these serum specimens. The combined antibody and antigen tests for invasive aspergillosis carry a sensitivity of 86.7% (13 of 15). The specificities of the tests are high since none of the 138 control sera, including 100 from normal blood donors, 20 from patients with penicilliosis marneffei, 6 from patients with candidemia, 8 from patients with typhoid fever, and 4 from patients with melioidosis, was positive by either test. In conclusion, the combined Afmplp antibody and antigen tests are highly sensitive and specific for A. fumigatus invasive aspergillosis.
Persistent Identifierhttp://hdl.handle.net/10722/79171
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorChan, CMen_HK
dc.contributor.authorLeung, ASPen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorChe, XYen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorCao, Len_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:51:27Z-
dc.date.available2010-09-06T07:51:27Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2002, v. 40 n. 11, p. 4382-4387en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79171-
dc.description.abstractMannoproteins are important and abundant structural components of fungal cell walls. The AFMP1 gene encodes a cell wall galactomannoprotein of Aspergillusfumigatus. In the present study, we show that Afmplp is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Afmplp was capable of detecting this protein from the cell culture supernatant of A. fumigatus. The anti-Afmp1p antibody is specific since it fails to react with any protein from lysates of Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Penicillium marneffei, Candida albicans, Cryptococcus neoformans, Blastomyces dermatitidis, and Histoplasma capsulatum by Western blotting. In addition, this Afmp1p antigen-based ELISA is also specific for 4. fumigatus since the cell culture supernatants of the other eight fungi gave negative results. Finally, a clinical evaluation of sera from invasive aspergillosis patients indicates that 8 of 15 (53%) patients are Afmplp antigen test positive. Furthermore, an Afmplp antibody test was performed with these serum specimens. The combined antibody and antigen tests for invasive aspergillosis carry a sensitivity of 86.7% (13 of 15). The specificities of the tests are high since none of the 138 control sera, including 100 from normal blood donors, 20 from patients with penicilliosis marneffei, 6 from patients with candidemia, 8 from patients with typhoid fever, and 4 from patients with melioidosis, was positive by either test. In conclusion, the combined Afmplp antibody and antigen tests are highly sensitive and specific for A. fumigatus invasive aspergillosis.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.subject.meshAntibodies, Fungal - blood - diagnostic use - immunologyen_HK
dc.subject.meshAntibody Specificityen_HK
dc.subject.meshAntigens, Fungal - analysis - blood - immunologyen_HK
dc.subject.meshAspergillosis - diagnosis - microbiologyen_HK
dc.subject.meshAspergillus fumigatus - chemistry - immunology - isolation & purificationen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Wall - chemistryen_HK
dc.subject.meshCulture Media, Conditioned - chemistryen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMembrane Glycoproteins - analysis - blood - immunologyen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.titleDetection of cell wall galactomannoprotein Afmp1p in culture supernatants of Aspergillus fumigatus and in sera of aspergillosis patientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=40&spage=4382&epage=4387&date=2002&atitle=Detection+of+cell+wall+galactomannoprotein+Afmp1p+in+culture+supernatants+of+Aspergillus+fumigatus+and+in+sera+of+Aspergillosis+patients+en_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1128/JCM.40.11.4382-4387.2002en_HK
dc.identifier.pmid12409437-
dc.identifier.scopuseid_2-s2.0-0036840269en_HK
dc.identifier.hkuros80261en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036840269&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue11en_HK
dc.identifier.spage4382en_HK
dc.identifier.epage4387en_HK
dc.identifier.isiWOS:000179053200091-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridLeung, ASP=7403012546en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridCao, L=7401637818en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats