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Article: Human herpes virus-6 infection in marrow graft recipients: Role in pathogenesis of graft-versus-host disease

TitleHuman herpes virus-6 infection in marrow graft recipients: Role in pathogenesis of graft-versus-host disease
Authors
KeywordsGVHD
Herpes virus
Marrow graft
Issue Date1995
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bmt
Citation
Bone Marrow Transplantation, 1995, v. 16 n. 6, p. 777-782 How to Cite?
AbstractTo investigate the hypothesis that target organ infection with human herpes virus-6 (HHV-6) exacerbates the clinical severity of GVHD, skin and rectal biopsies from 34 allogeneic bone marrow transplant (BMT) recipients and 23 comparative autologous recipients were studied. Biopsies and heparinised blood samples were obtained from all patients prior to and at regular intervals after BMT, and whenever GVHD was suspected. HHV-6 antigen was detected in cryostat sections by immunohistochemistry, and HHV-6 DNA in peripheral blood leucocytes (PBL) and biopsies by nested PCR. Twenty-eight (90%) of the 31 patients who engrafted developed clinical GVHD, which was mild in five, moderately severe in nine and severe in 14. Overall, HHV-6 DNA was detected in PBL in 74% of autologous recipients and 76% of allogeneic recipients, and in biopsy tissue in 48% of autos and 71% of allos. However, HHV-6 DNA was detected in skin and/or rectal biopsies more frequently in allogeneic recipients with severe GVHD (92%) than in those with either moderate (55%) or mild GVHD (22%), suggesting an association (P = 0.004) between HHV-6 DNA in biopsy tissue and GVHD severity. A significant linear trend (P = 0.03) was identified between detection of HHV-6 DNA in biopsy tissue obtained prior to or concomitant with the onset of GVHD and increased GVHD severity, suggesting that HHV-6 was causally linked to GVHD rather than reactivated as a consequence of GVHD therapy. Thus this study supports a role for HHV-6 in the initiation and/or exacerbation of GVHD, and suggests that the presence of HHV-6 DNA in the skin or rectum may be a factor in determining GVHD severity. If confirmed, these findings may have implications for the management of allogeneic BMT recipients.
Persistent Identifierhttp://hdl.handle.net/10722/79137
ISSN
2015 Impact Factor: 3.636
2015 SCImago Journal Rankings: 1.585
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAppleton, ALen_HK
dc.contributor.authorSviland, Len_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorTaylor, CEen_HK
dc.contributor.authorWilkes, Jen_HK
dc.contributor.authorGreen, MAen_HK
dc.contributor.authorPearson, ADJen_HK
dc.contributor.authorKelly, PJen_HK
dc.contributor.authorMalcolm, AJen_HK
dc.contributor.authorProctor, SJen_HK
dc.contributor.authorHamilton, PJen_HK
dc.contributor.authorCant, AJen_HK
dc.date.accessioned2010-09-06T07:51:02Z-
dc.date.available2010-09-06T07:51:02Z-
dc.date.issued1995en_HK
dc.identifier.citationBone Marrow Transplantation, 1995, v. 16 n. 6, p. 777-782en_HK
dc.identifier.issn0268-3369en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79137-
dc.description.abstractTo investigate the hypothesis that target organ infection with human herpes virus-6 (HHV-6) exacerbates the clinical severity of GVHD, skin and rectal biopsies from 34 allogeneic bone marrow transplant (BMT) recipients and 23 comparative autologous recipients were studied. Biopsies and heparinised blood samples were obtained from all patients prior to and at regular intervals after BMT, and whenever GVHD was suspected. HHV-6 antigen was detected in cryostat sections by immunohistochemistry, and HHV-6 DNA in peripheral blood leucocytes (PBL) and biopsies by nested PCR. Twenty-eight (90%) of the 31 patients who engrafted developed clinical GVHD, which was mild in five, moderately severe in nine and severe in 14. Overall, HHV-6 DNA was detected in PBL in 74% of autologous recipients and 76% of allogeneic recipients, and in biopsy tissue in 48% of autos and 71% of allos. However, HHV-6 DNA was detected in skin and/or rectal biopsies more frequently in allogeneic recipients with severe GVHD (92%) than in those with either moderate (55%) or mild GVHD (22%), suggesting an association (P = 0.004) between HHV-6 DNA in biopsy tissue and GVHD severity. A significant linear trend (P = 0.03) was identified between detection of HHV-6 DNA in biopsy tissue obtained prior to or concomitant with the onset of GVHD and increased GVHD severity, suggesting that HHV-6 was causally linked to GVHD rather than reactivated as a consequence of GVHD therapy. Thus this study supports a role for HHV-6 in the initiation and/or exacerbation of GVHD, and suggests that the presence of HHV-6 DNA in the skin or rectum may be a factor in determining GVHD severity. If confirmed, these findings may have implications for the management of allogeneic BMT recipients.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bmten_HK
dc.relation.ispartofBone Marrow Transplantationen_HK
dc.subjectGVHDen_HK
dc.subjectHerpes virusen_HK
dc.subjectMarrow graften_HK
dc.titleHuman herpes virus-6 infection in marrow graft recipients: Role in pathogenesis of graft-versus-host diseaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-3369&volume=16&spage=777&epage=782&date=1995&atitle=Human+herpes+virus-6+infection+in+marrow+graft+recipients:+role+in+pathogenesis+of+graft-versus-host+diseaseen_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8750269-
dc.identifier.scopuseid_2-s2.0-0029615417en_HK
dc.identifier.hkuros14628en_HK
dc.identifier.volume16en_HK
dc.identifier.issue6en_HK
dc.identifier.spage777en_HK
dc.identifier.epage782en_HK
dc.identifier.isiWOS:A1995TK63400009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridAppleton, AL=7005827700en_HK
dc.identifier.scopusauthoridSviland, L=7003823044en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridTaylor, CE=7404822545en_HK
dc.identifier.scopusauthoridWilkes, J=7102513218en_HK
dc.identifier.scopusauthoridGreen, MA=16143678900en_HK
dc.identifier.scopusauthoridPearson, ADJ=7401994312en_HK
dc.identifier.scopusauthoridKelly, PJ=7403399402en_HK
dc.identifier.scopusauthoridMalcolm, AJ=7103265161en_HK
dc.identifier.scopusauthoridProctor, SJ=7102137191en_HK
dc.identifier.scopusauthoridHamilton, PJ=35554560400en_HK
dc.identifier.scopusauthoridCant, AJ=7006244214en_HK

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